Effect of carbohydrate restriction on oxidative stress response to high-intensity resistance exercise
Corresponding Author
Matthew John McAllister
Metabolic and Applied Physiology Lab, Department of Health and Human Performance, Texas State University, San Marcos, Texas
Correspondence
Matthew John McAllister, Department of Health & Human Performance, Texas State University, San Marcos, TX 78666.
Email: [email protected]
Search for more papers by this authorSteven A. Basham
Applied Physiology Lab, Department of Kinesiology, Mississippi State University, Starkville, Mississippi
Author Steven Basham was affiliated with Mississippi State University at the time of data collection and is currently employed by the Gatorade Sports Science Institute, a division of PepsiCo, Inc.
Search for more papers by this authorJohnEric W. Smith
Applied Physiology Lab, Department of Kinesiology, Mississippi State University, Starkville, Mississippi
Search for more papers by this authorBrent J. Fountain
Department of Food Science, Nutrition, and Health Promotion, Mississippi State University, Starkville, Mississippi
Search for more papers by this authorBenjamin M. Krings
Department of Health and Human Performance, University of Wisconsin-Platteville, Platteville, Wisconsin
Search for more papers by this authorHunter S. Waldman
Human Performance Lab, Department of Kinesiology, University of North Alabama, Florence, Alabama
Search for more papers by this authorCorresponding Author
Matthew John McAllister
Metabolic and Applied Physiology Lab, Department of Health and Human Performance, Texas State University, San Marcos, Texas
Correspondence
Matthew John McAllister, Department of Health & Human Performance, Texas State University, San Marcos, TX 78666.
Email: [email protected]
Search for more papers by this authorSteven A. Basham
Applied Physiology Lab, Department of Kinesiology, Mississippi State University, Starkville, Mississippi
Author Steven Basham was affiliated with Mississippi State University at the time of data collection and is currently employed by the Gatorade Sports Science Institute, a division of PepsiCo, Inc.
Search for more papers by this authorJohnEric W. Smith
Applied Physiology Lab, Department of Kinesiology, Mississippi State University, Starkville, Mississippi
Search for more papers by this authorBrent J. Fountain
Department of Food Science, Nutrition, and Health Promotion, Mississippi State University, Starkville, Mississippi
Search for more papers by this authorBenjamin M. Krings
Department of Health and Human Performance, University of Wisconsin-Platteville, Platteville, Wisconsin
Search for more papers by this authorHunter S. Waldman
Human Performance Lab, Department of Kinesiology, University of North Alabama, Florence, Alabama
Search for more papers by this authorAbstract
Ketogenic diets consisting of >70% dietary intake from lipids may induce favorable shifts in the redox environment such as reduced markers of oxidative stress (OS). However, a diet with such low dietary carbohydrate intake is difficult to sustain for long periods of time. This study investigated the effects of a low-carbohydrate, high-fat (LCHF) diet on markers of OS before and after high-intensity resistance exercise (HIRE). The HIRE protocol involved five rounds of back squat, deadlift, and bench press completed in respective order and in a descending ladder (10, 8, 6, 4, 2 repetitions) with minimal to no rest. The LCHF diet was 15 days of >50% of calories from fat, ≥25% of calories from protein, and ≤25% of calories from carbohydrates. Blood was sampled before exercise, immediately post-exercise, and 60 minutes post-exercise and analyzed for total antioxidant capacity (TAC), tumor necrosis factor-α (TNF-α), malondialdehyde (MDA), superoxide dismutase (SOD), and glucose. The exercise protocol increased (P < .05) plasma levels of glucose, TNF-α, and MDA. There was a significant increase in MDA from pre to post exercise following the western diet but not the LCHF condition. The LCHF diet did not significantly impact any other blood marker of OS.
CONFLICT OF INTEREST
The authors have no conflict of interest in relation to this manuscript.
REFERENCES
- 1Fisher-Wellman K, Bloomer RJ. Acute exercise and oxidative stress: a 30 year history. Dyn Med. 2009; 8: 1.
- 2Huang Y, Li W, Su ZY, Kong A. The complexity of the Nrf2 pathway: beyond the antioxidant response. J Nutr Biochem. 2015; 26: 1401-1413.
- 3Sullivan PG, Rippy NA, Dorenbos K, Concepcion RC, Agarwal AK, Rho JM. The ketogenic diet increases mitochondrial uncoupling protein levels and activity. Ann Neurol. 2004; 55: 576-580.
- 4Bough KJ, Wetherington J, Hassel B, et al. Mitochondrial biogenesis in the anticonvulsant mechanism of the ketogenic diet. Ann of Neurol. 2006; 60: 223-235.
- 5Jarrett SG, Milder JB, Liang LP, Patel M. The ketogenic diet increases mitochondrial glutathione levels. J Neurochem. 2008; 106: 1044-1051.
- 6Hyatt HW, Kephart WC, Holland AM, et al. A ketogenic diet in rodents elicits improved mitochondrial adaptations in response to resistance exercise training compared to an isocaloric western diet. Front Physiol. 2016; 7: 1-9.
- 7Nazarewicz RR, Ziolkowski W, Vaccaro PS, Ghafourifar P. Effect of short-term ketogenic diet on redox status of human blood. Rejuvenation Res. 2007; 10: 435-440.
- 8Waldman HS, Krings BM, Basham SA, Smith J, Fountain BJ, McAllister MJ. Effects of a 15-day low carbohydrate, high-fat diet in resistance-trained men. J Strength Cond Res. 2018; 32: 3103-3111.
- 9Volek JS, Sharman MJ, Gómez AL, et al. Comparison of a very low-carbohydrate and low-fat diet on fasting lipids, LDL subclasses, insulin resistance, and postprandial lipemic responses in overweight women. J Am Coll Nutr. 2004; 23: 177-184.
- 10Volek JS, Sharman MJ, Love DM, et al. Body composition and hormonal responses to a carbohydrate-restricted diet. Metabolism. 2002; 51: 864-870.
- 11Azizbeigi K, Azarbayjani MA, Atashak S, Stannard SR. Effect of moderate and high resistance training intensity on indices of inflammatory and oxidative stress. Res in Sports Med. 2015; 23: 73-87.
- 12Kliszczewicz B, John QC, Daniel BL, Gretchen OD, Michael ER, Kyle TJ. Acute exercise and oxidative stress: CrossFit™ vs. treadmill bout. J Hum Kinet. 2015; 47: 81-90.
- 13 Medicine ACoS. ACSM's guidelines for exercise testing and prescription. Philadelphia, PA: Lippincott Williams & Wilkins; 2013.
- 14Foster GD, Wyatt HR, Hill JO, et al. A randomized trial of a low-carbohydrate diet for obesity. N Engl J Med. 2003; 348: 2082-2090.
- 15McAllister M, Webb H, Tidwell D, et al. Exogenous carbohydrate reduces cortisol response from combined mental and physical stress. Int J Sports Med. 2016; 37: 1159-1165.
- 16Yagi K. Simple assay for the level of total lipid peroxides in serum or plasma. Methods Mol Biol. 1998; 108: 101-106.
- 17Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci. 1979; 1993(84): 407-412.
- 18Marklund S. Distribution of CuZn superoxide dismutase and Mn superoxide dismutase in human tissues and extracellular fluids. Acta Physiol Scand Suppl. 1980; 492: 19-23.
- 19Kosmidou I, Vassilakopoulos T, Xagorari A, Zakynthinos S, Papapetropoulos A, Roussos C. Production of interleukin-6 by skeletal myotubes: role of reactive oxygen species. Am J Respir Cell Mol Biol. 2002; 26: 587-593.
- 20Yasunari K, Maeda K, Nakamura M, Yoshikawa J. Oxidative stress in leukocytes is a possible link between blood pressure, blood glucose, and C-reacting protein. Hypertension. 2002; 39: 777-780.
- 21Wei-Chuan T, Yi-Heng L, Chih-Chan L, Ting-Hsing C, Jyh-Hong C. Effects of oxidative stress on endothelial function after a high-fat meal. Clin Sci. 2004; 106: 315-319.
- 22Bray GA, Popkin BM. Dietary fat intake does affect obesity! Am J Clin Nutr. 1998; 68: 1157-1173.
- 23Milagro FI, Campión J, Martínez JA. Weight gain induced by high-fat feeding involves increased liver oxidative stress. Obesity. 2006; 14: 1118-1123.
- 24Buettner R, Schölmerich J, Bollheimer LC. High-fat diets: modeling the metabolic disorders of human obesity in rodents. Obesity. 2007; 15: 798-808.
- 25Rhyu HS, Cho SY. The effect of weight loss by ketogenic diet on the body composition, performance-related physical fitness factors and cytokines of Taekwondo athletes. J Exer Rehab. 2014; 10: 326.
- 26Maalouf M, Sullivan PG, Davis L, Kim DY, Rho JM. Ketones inhibit mitochondrial production of reactive oxygen species production following glutamate excitotoxicity by increasing NADH oxidation. Neuroscience. 2007; 145: 256-264.
- 27Kim DY, Davis LM, Sullivan PG, et al. Ketone bodies are protective against oxidative stress in neocortical neurons. J Neurochem. 2007; 101: 1316-1326.
- 28Robertson J, Maughan R, Duthie G, Morrice P. Increased blood antioxidant systems of runners in response to training load. Clin Sci. 1991; 80: 611-618.
- 29Seifi-skishahr F, Damirchi A, Farjaminezhad M, Babaei P. Physical training status determines oxidative stress and redox changes in response to an acute aerobic exercise. Biochem Res Int. 2016; 2016: 1-9.
- 30Bloomer RJ, Goldfarb AH. Anaerobic exercise and oxidative stress: a review. Can J Appl Physiol. 2004; 29: 245-263.
- 31Bloomer RJ, Schilling BK, Karlage RE, Ledoux MS, Pfeiffer RF, Callegari J. Effect of resistance training on blood oxidative stress in Parkinson disease. Med Sci Sports Exerc. 2008; 40: 1385-1389.
- 32Effting PS, Brescianini SMS, Sorato HR, et al. Resistance Exercise Modulates Oxidative Stress Parameters and TNF-α Content in the Heart of Mice with Diet-Induced Obesity. Arq Bras Cardiol. 2019; 112: 545–552.
