Addition of platelet-rich plasma supports immune modulation and improved mechanical integrity in Alloderm mesh for ventral hernia repair in a rat model
Corresponding Author
Joseph S. Fernandez-Moure
Department of Surgery, Duke University School of Medicine, Houston, Texas, USA
Correspondence
Joseph S. Fernandez-Moure, Division of Trauma, Acute and Critical Care Surgery, Department of Surgery, Duke University Medical Center, DUMC 2837, 2301 Erwin Road, Durham, NC 27710, USA.
Email: [email protected]
Search for more papers by this authorJeffrey L. Van Eps
Department of Surgery, Houston Methodist Hospital, Houston, Texas, USA
Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA
Search for more papers by this authorJacob C. Scherba
Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA
Search for more papers by this authorIman K. Yazdi
Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA
Renal Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA
Search for more papers by this authorAndrew Robbins
Department of Orthopedic Surgery, Houston Methodist Hospital, Houston, Texas, USA
Search for more papers by this authorFernando Cabrera
Baylor College of Medicine, Houston, Texas, USA
Search for more papers by this authorCory J. Vatsaas
Department of Surgery, Duke University School of Medicine, Houston, Texas, USA
Search for more papers by this authorMichael Moreno
Texas A&M College of Medicine, Bryan, Texas, USA
Search for more papers by this authorBradley K. Weiner
Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA
Department of Orthopedic Surgery, Houston Methodist Hospital, Houston, Texas, USA
Search for more papers by this authorEnnio Tasciotti
Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA
Search for more papers by this authorCorresponding Author
Joseph S. Fernandez-Moure
Department of Surgery, Duke University School of Medicine, Houston, Texas, USA
Correspondence
Joseph S. Fernandez-Moure, Division of Trauma, Acute and Critical Care Surgery, Department of Surgery, Duke University Medical Center, DUMC 2837, 2301 Erwin Road, Durham, NC 27710, USA.
Email: [email protected]
Search for more papers by this authorJeffrey L. Van Eps
Department of Surgery, Houston Methodist Hospital, Houston, Texas, USA
Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA
Search for more papers by this authorJacob C. Scherba
Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA
Search for more papers by this authorIman K. Yazdi
Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA
Renal Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA
Search for more papers by this authorAndrew Robbins
Department of Orthopedic Surgery, Houston Methodist Hospital, Houston, Texas, USA
Search for more papers by this authorFernando Cabrera
Baylor College of Medicine, Houston, Texas, USA
Search for more papers by this authorCory J. Vatsaas
Department of Surgery, Duke University School of Medicine, Houston, Texas, USA
Search for more papers by this authorMichael Moreno
Texas A&M College of Medicine, Bryan, Texas, USA
Search for more papers by this authorBradley K. Weiner
Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA
Department of Orthopedic Surgery, Houston Methodist Hospital, Houston, Texas, USA
Search for more papers by this authorEnnio Tasciotti
Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA
Search for more papers by this authorAbstract
The recurrence of ventral hernias continues to be a problem faced by surgeons, in spite of efforts toward implementing novel repair techniques and utilizing different materials to promote healing. Cadaveric acellular dermal matrices (Alloderm) have shown some promise in numerous surgical subspecialties, but these meshes still suffer from subsequent failure and necessitation of re-intervention. Here, it is demonstrated that the addition of platelet rich plasma to Alloderm meshes temporally modulates both the innate and cytotoxic inflammatory responses to the implanted material. This results in decreased inflammatory cytokine production at early time points, decreased matrix metalloproteinase expression, and decreased CD8+ T cell infiltration. Collectively, these immune effects result in a healing phenotype that is free from mesh thinning and characterized by increased material stiffness.
CONFLICT OF INTEREST
The authors have no conflicts of interest to declare.
AUTHOR CONTRIBUTIONS
Joseph S. Fernandez-Moure: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Writing—original draft; Jeffrey L. Van Eps: Data curation, Formal analysis; Jacob C. Scherba: Writing—revisions and subsequent drafts; Iman K. Yazdi: Data curation, Formal analysis; Andrew Robbins: Formal analysis, writing; Data curation, Formal analysis; Fernando Cabrera: Formal analysis, writing; Cory Vatsaas: writing, analysis, revisions; Michael Moreno: Formal analysis and writing; Bradley K. Weiner: Formal analysis and writing; Ennio Tasciotti: Project administration, Methodology, Supervision.
Supporting Information
| Filename | Description |
|---|---|
| term3156-sup-0001-fig_s1.tif2.5 MB | Supplementary Material |
| term3156-sup-0002-fig_s2.tif2.6 MB | Supplementary Material |
| term3156-sup-0003-fig_s3.tif2.3 MB | Supplementary Material |
| term3156-sup-0004-fig_s4.tif551.8 KB | Supplementary Material |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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