Promotion of dermal regeneration using pullulan/gelatin porous skin substitute
Nan Cheng
Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada
Search for more papers by this authorCorresponding Author
Marc G. Jeschke
Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada
Institute of Medical Science, University of Toronto, Toronto, ON, Canada
Department of Surgery, University of Toronto, Toronto, ON, Canada
Department of Immunology, University of Toronto, Toronto, ON, Canada
Ross-Tilley Burn Centre, Sunnybrook Health Sciences Centre, Toronto, ON, Canada
Correspondence
Saeid Amini Nik, Sunnybrook Research Institute, University of Toronto, Toronto, ON M4N 3M5, Canada.
Email: [email protected]
Marc G Jeschke, Sunnybrook Research Institute, University of Toronto, Toronto, ON M4N 3M5, Canada.
Email: [email protected]
Search for more papers by this authorMohammadali Sheikholeslam
Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada
Search for more papers by this authorAndrea-Kaye Datu
Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada
Search for more papers by this authorHwan Hee Oh
Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada
Search for more papers by this authorCorresponding Author
Saeid Amini-Nik
Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada
Department of Surgery, University of Toronto, Toronto, ON, Canada
Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada
Correspondence
Saeid Amini Nik, Sunnybrook Research Institute, University of Toronto, Toronto, ON M4N 3M5, Canada.
Email: [email protected]
Marc G Jeschke, Sunnybrook Research Institute, University of Toronto, Toronto, ON M4N 3M5, Canada.
Email: [email protected]
Search for more papers by this authorNan Cheng
Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada
Search for more papers by this authorCorresponding Author
Marc G. Jeschke
Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada
Institute of Medical Science, University of Toronto, Toronto, ON, Canada
Department of Surgery, University of Toronto, Toronto, ON, Canada
Department of Immunology, University of Toronto, Toronto, ON, Canada
Ross-Tilley Burn Centre, Sunnybrook Health Sciences Centre, Toronto, ON, Canada
Correspondence
Saeid Amini Nik, Sunnybrook Research Institute, University of Toronto, Toronto, ON M4N 3M5, Canada.
Email: [email protected]
Marc G Jeschke, Sunnybrook Research Institute, University of Toronto, Toronto, ON M4N 3M5, Canada.
Email: [email protected]
Search for more papers by this authorMohammadali Sheikholeslam
Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada
Search for more papers by this authorAndrea-Kaye Datu
Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada
Search for more papers by this authorHwan Hee Oh
Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada
Search for more papers by this authorCorresponding Author
Saeid Amini-Nik
Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada
Department of Surgery, University of Toronto, Toronto, ON, Canada
Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada
Correspondence
Saeid Amini Nik, Sunnybrook Research Institute, University of Toronto, Toronto, ON M4N 3M5, Canada.
Email: [email protected]
Marc G Jeschke, Sunnybrook Research Institute, University of Toronto, Toronto, ON M4N 3M5, Canada.
Email: [email protected]
Search for more papers by this authorAbstract
Tissue-engineered dermal substitutes represent a promising approach to improve wound healing and provide more sufficient regeneration, compared with current clinical standards on care of large wounds, early excision, and grafting of autografts. However, inadequate regenerative capacity, impaired regeneration/degradation profile, and high cost of current commercial tissue-engineered dermal regeneration templates hinder their utilization, and the development of an efficient and cost-effective tissue-engineered dermal substitute remains a challenge. Inspired from our previously reported data on a pullulan/gelatin scaffold, here we present a new generation of a porous pullulan/gelatin scaffold (PG2) served as a dermal substitute with enhanced chemical and structural characteristics. PG2 shows excellent biocompatibility (viability, migration, and proliferation), assessed by in vitro incorporation of human dermal fibroblasts in comparison with the Integra® dermal regeneration template (Control). When applied on a mouse full-thickness excisional wound, PG2 shows rapid scaffold degradation, more granulation tissue, more collagen deposition, and more cellularity in comparison with Control at 20 days post surgery. The faster degradation is likely due to the enhanced recruitment of inflammatory macrophages to the scaffold from the wound bed, and that leads to earlier maturation of granulation tissue with less myofibroblastic cells. Collectively, our data reveal PG2's characteristics as an applicable dermal substitute with excellent dermal regeneration, which may attenuate scar formation.
CONFLICT OF INTEREST
The authors have declared that there is no conflict of interest.
Supporting Information
| Filename | Description |
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| term2946-sup-0001-Figure_S1.tifTIFF image, 2.9 MB |
Figure S1. Supporting information |
| term2946-sup-0002-Figure_S2.tifTIFF image, 9.1 MB |
Figure S2. Supporting information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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