Induction of differentiation in rat C6 glioma cells with Saikosaponins
Yan-Jyu Tsai
Department of Pharmacology, Institute of Medical Research, Taipei Medical College, Taipei, Taiwan, R.O.C.
Search for more papers by this authorI-Ling Chen
Department of Pharmacology, Institute of Medical Research, Taipei Medical College, Taipei, Taiwan, R.O.C.
Search for more papers by this authorLin-Yea Horng
Institute of Biopharmaceutical Science, National Yang-Ming University, Taipei, Taiwan, R.O.C.
Search for more papers by this authorCorresponding Author
Dr Rong-Tsun Wu
Institute of Biopharmaceutical Science, National Yang-Ming University, Taipei, Taiwan, R.O.C.
Institute of Biopharmaceutical Science, National Yang-Ming University, Taipei, Taiwan, R.O.C.Search for more papers by this authorYan-Jyu Tsai
Department of Pharmacology, Institute of Medical Research, Taipei Medical College, Taipei, Taiwan, R.O.C.
Search for more papers by this authorI-Ling Chen
Department of Pharmacology, Institute of Medical Research, Taipei Medical College, Taipei, Taiwan, R.O.C.
Search for more papers by this authorLin-Yea Horng
Institute of Biopharmaceutical Science, National Yang-Ming University, Taipei, Taiwan, R.O.C.
Search for more papers by this authorCorresponding Author
Dr Rong-Tsun Wu
Institute of Biopharmaceutical Science, National Yang-Ming University, Taipei, Taiwan, R.O.C.
Institute of Biopharmaceutical Science, National Yang-Ming University, Taipei, Taiwan, R.O.C.Search for more papers by this authorAbstract
The effects of saikosaponins (a, b1, b2, c, d), isolated from Bupleurum Radix, on the induction of differentiation in rat C6 glioma cells were studied. Saikosaponins a and d were shown to inhibit cell proliferation and alter cell morphology. In addition to cytostasis, the enzymatic activities of glutamine synthetase (GS) and 2′,3′-cyclic nucleotide 3′-phosphohydrolase (CNP) were also noticeably increased after treatment with saikosaponin a. Nevertheless, saikosaponin d only showed an increase of GS activity, no significant changes in CNP activity were found. These results suggest that saikosaponin a can induce the differentiation of C6 glioma cells into astrocytes and/or oligodendrocytes, but saikosaponin d can only induce the differentiation of C6 glioma cells into astrocytes. Copyright © 2002 John Wiley & Sons, Ltd.
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