Chemical Composition and Antioxidant, Antinociceptive, and Anti-inflammatory Activities of Four Amazonian Byrsonima Species
Fernanda Guilhon-Simplicio
Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, 69070-000 Amazonas, Brazil
Search for more papers by this authorTallita Marques Machado
Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, 69070-000 Amazonas, Brazil
Search for more papers by this authorLeandro Flores do Nascimento
Center of Biological Sciences, Federal University of Santa Catarina, Florianópolis, 88040-900 Santa Catarina, Brazil
Search for more papers by this authorRayanne da Silva Souza
Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, 69070-000 Amazonas, Brazil
Search for more papers by this authorHector Henrique Ferreira Koolen
Metabolomics and Mass Spectrometry Research Group, Amazonas State University, Manaus, 69065-001 Amazonas, Brazil
Search for more papers by this authorFelipe Moura Araújo da Silva
Department of Chemistry, Federal University of Amazonas, Manaus, 69070-000 Amazonas, Brazil
Search for more papers by this authorLeonard Domingo Rosales Acho
Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, 69070-000 Amazonas, Brazil
Search for more papers by this authorAdair Roberto Soares dos Santos
Center of Biological Sciences, Federal University of Santa Catarina, Florianópolis, 88040-900 Santa Catarina, Brazil
Search for more papers by this authorPaul Cos
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Wilrijk, 2610 Antwerp, Belgium
Search for more papers by this authorMaria de Meneses Pereira
Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, 69070-000 Amazonas, Brazil
Search for more papers by this authorCorresponding Author
Emerson Silva Lima
Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, 69070-000 Amazonas, Brazil
Correspondence to: Emerson Silva Lima, Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Rodrigo Otávio, 3000, Aleixo, 69070-000 Manaus, Amazonas, Brazil.
E-mail: [email protected]
Search for more papers by this authorFernanda Guilhon-Simplicio
Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, 69070-000 Amazonas, Brazil
Search for more papers by this authorTallita Marques Machado
Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, 69070-000 Amazonas, Brazil
Search for more papers by this authorLeandro Flores do Nascimento
Center of Biological Sciences, Federal University of Santa Catarina, Florianópolis, 88040-900 Santa Catarina, Brazil
Search for more papers by this authorRayanne da Silva Souza
Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, 69070-000 Amazonas, Brazil
Search for more papers by this authorHector Henrique Ferreira Koolen
Metabolomics and Mass Spectrometry Research Group, Amazonas State University, Manaus, 69065-001 Amazonas, Brazil
Search for more papers by this authorFelipe Moura Araújo da Silva
Department of Chemistry, Federal University of Amazonas, Manaus, 69070-000 Amazonas, Brazil
Search for more papers by this authorLeonard Domingo Rosales Acho
Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, 69070-000 Amazonas, Brazil
Search for more papers by this authorAdair Roberto Soares dos Santos
Center of Biological Sciences, Federal University of Santa Catarina, Florianópolis, 88040-900 Santa Catarina, Brazil
Search for more papers by this authorPaul Cos
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Wilrijk, 2610 Antwerp, Belgium
Search for more papers by this authorMaria de Meneses Pereira
Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, 69070-000 Amazonas, Brazil
Search for more papers by this authorCorresponding Author
Emerson Silva Lima
Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, 69070-000 Amazonas, Brazil
Correspondence to: Emerson Silva Lima, Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Rodrigo Otávio, 3000, Aleixo, 69070-000 Manaus, Amazonas, Brazil.
E-mail: [email protected]
Search for more papers by this authorAbstract
Species of the Byrsonima genus are widely used in Brazil, especially for the treatment of gastrointestinal disorders. However, species from the Amazonian region are still poorly studied. Thus, we studied the antioxidant, antinociceptive, and anti-inflammatory activities of for Amazonian species, Byrsonima crispa, Byrsonima duckeana, Byrsonima garcibarrigae, and Byrsonima incarnata. Phenolic composition was determined by chemical and chromatographic methods. The aqueous extracts were evaluated in DPPH•, ABTS+•, and superoxide (O2•−) tests, LPS-activated macrophage assay, and formalin test. All species contained a high phenolic and flavonoid content. We identified 15 phenolic compounds, including phenolic acids, hydroxycinnamic acids, flavonoids, and catechins. The extracts showed high antioxidant activity and were more active than quercetin at inhibiting nitric oxide release in the LPS-activated macrophage assay. B. duckeana and B. garcibarrigae showed higher in vivo antinociceptive and anti-inflammatory activities. B. garcibarrigae presented significant effect on the early phase of the formalin test, pointing to an antinociceptive mechanism distinct from traditional anti-inflammatory medicines. In conclusion, the pharmacological potential of these species is closely related to their flavonoid-rich chemical composition, which seems to act through antioxidant mechanisms. Copyright © 2017 John Wiley & Sons, Ltd.
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