Proteomic Analysis of Protective Effects of Polysaccharides from Salvia miltiorrhiza Against Immunological Liver Injury in Mice
Xue-Gang Sun
The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
These authors contributed to this work equally.Search for more papers by this authorXiu-Qiong Fu
The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
These authors contributed to this work equally.Search for more papers by this authorHong-Bing Cai
The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
These authors contributed to this work equally.Search for more papers by this authorQiang Liu
The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
Search for more papers by this authorChun-Hua Li
The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
Search for more papers by this authorYa-Wei Liu
Key Laboratory of Functional Proteomics of Guangdong Province, Department of Pathophysiology, Southern Medical University, Guangzhou, Guangdong Province, China
Search for more papers by this authorYing-Jia Li
Ultrasound Department, Nan Fang Hospital, Guangzhou, Guangdong Province, China
Search for more papers by this authorZhi-Feng Liu
Guangzhou General Hospital, Guangzhou Military Region PLA, Guangzhou, Guangdong Province, China
Search for more papers by this authorYu-Hong Song
The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
Search for more papers by this authorCorresponding Author
Zhi-Ping Lv
The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
Zhi-Ping Lv, No. 1838, Guang Zhou Da Dao Bei Road, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, 510515, China.
E-mail: [email protected]
Search for more papers by this authorXue-Gang Sun
The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
These authors contributed to this work equally.Search for more papers by this authorXiu-Qiong Fu
The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
These authors contributed to this work equally.Search for more papers by this authorHong-Bing Cai
The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
These authors contributed to this work equally.Search for more papers by this authorQiang Liu
The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
Search for more papers by this authorChun-Hua Li
The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
Search for more papers by this authorYa-Wei Liu
Key Laboratory of Functional Proteomics of Guangdong Province, Department of Pathophysiology, Southern Medical University, Guangzhou, Guangdong Province, China
Search for more papers by this authorYing-Jia Li
Ultrasound Department, Nan Fang Hospital, Guangzhou, Guangdong Province, China
Search for more papers by this authorZhi-Feng Liu
Guangzhou General Hospital, Guangzhou Military Region PLA, Guangzhou, Guangdong Province, China
Search for more papers by this authorYu-Hong Song
The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
Search for more papers by this authorCorresponding Author
Zhi-Ping Lv
The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
Zhi-Ping Lv, No. 1838, Guang Zhou Da Dao Bei Road, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, 510515, China.
E-mail: [email protected]
Search for more papers by this authorAbstract
This study was designed to investigate mechanisms of the protective effects of Salvia miltiorrhiza polysaccharide (SMPS) against lipopolysaccharide (LPS)-induced immunological liver injury (ILI) in Bacille Calmette-Guérin (BCG)-primed mice. Two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis showed that three proteins are down-regulated and six proteins are up-regulated by SMPS. SMPS reduces the degree of liver injury by up-regulating the enzymes of the citric acid cycle, namely malate dehydrogenase (MDH) and 2-oxoglutarate dehydrogenase complex. LPS significantly increases nuclear factor kappa B (NF-κB) activation, inducible nitric oxide synthase (iNOS) expression and MDA level in BCG primed mice liver, whereas SMPS treatment protects against the immunological liver injury through inhibition of the NF-κB activation by up-regulation of PRDX6 and the subsequent attenuation of lipid peroxidation, iNOS expression and inflammation. Copyright © 2011 John Wiley & Sons, Ltd.
REFERENCES
- But DY, Lai CL, Yuen MF. 2008. Natural history of hepatitis-related hepatocellular carcinoma. World J Gastroenterol 14: 1652–1656.
- Chowdhury I, Mo Y, Gao L, Kazi A, Fisher AB, Feinstein SI. 2009. Oxidant stress stimulates expression of the human peroxiredoxin 6 gene by a transcriptional mechanism involving an antioxidant response element. Free Radic Biol Med 46: 146–153.
- Devi KP, Sreepriya M, Balakrishna K, Devaki T. 2005. Protective effect of Premna tomentosa extract (L. verbanacae) on acetaminophen-induced mitochondrial dysfunction in rats. Mol Cell Biochem 272: 171–177.
- Eismann T, Huber N, Shin T et al. 2009. Peroxiredoxin-6 protects against mitochondrial dysfunction and liver injury during ischemia-reperfusion in mice. Am J Physiol Gastrointest Liver Physiol 296: G266–G274.
- Fatma N, Kubo E, Sen M et al. 2008. Peroxiredoxin 6 delivery attenuates TNF-alpha-and glutamate-induced retinal ganglion cell death by limiting ROS levels and maintaining Ca2+ homeostasis. Brain Res 1233: 63–78.
- Freeman WM, Hemby SE. 2004. Proteomics for protein expression profiling in neuroscience. Neurochem Res 29: 1065–1081.
- Heckert LL, Butler MH, Reimers JM, Albe KR, Wcenter BE. 1989. Purification and characterization of the 2-oxoglutarate dehydrogenase complex from Dictyostelium discoideum. J Gen Microbiol 135: 155–161.
- Kiemer AK, Hartung T, Huber C, Vollmar AM. 2003. Phyllanthus amarus has anti-inflammatory potential by inhibition of iNOS, COX-2, and cytokines via the NF-kappa B pathway. J Hepatol 38: 289–297.
- Kim HS, Manevich Y, Feinstein SI, Pak JH, Ho YS, Fisher AB. 2003. Induction of 1-cys peroxiredoxin expression by oxidative stress in lung epithelial cells. Am J Physiol Lung Cell Mol Physiol 285: L363–L369.
- Kim HS, Pak JH, Gonzales LW, Feinstein SI, Fisher AB. 2002. Regulation of 1-cys peroxiredoxin expression in lung epithelial cells. Am J Respir Cell Mol Biol 27: 227–233.
- Kobayashi S, Nishihira J, Watanabe S, Todo S. 1999. Prevention of lethal acute hepatic failure by antimacrophage migration inhibitory factor antibody in mice treated with bacille Calmette-Guerin and lipopolysaccharide. Hepatology 29: 1752–1759.
- Lee IS, Choi WH, Kim JY et al. 2008. Transcriptional regulation of the murine 1-cys peroxiredoxin gene by the B cell-specific activator protein, Pax5. J Cell Biochem 104: 465–476.
- Malhi H, Gores GJ. 2008. Cellular and molecular mechanisms of liver injury. Gastroenterology 134: 1641–1654.
- Na HJ, Lee G, Oh HY et al. 2006. 4-O-Methylgallic acid suppresses inflammation-associated gene expression by inhibition of redox-based NF-kappaB activation. Int Immunopharmacol 6: 1597–1608.
- Nagai H, Yakuo I, Yamada H et al. 1988. Liver injury model in mice for immunopharmacological study. Jpn J Pharmacol 46: 247–254.
- Roede JR, Orlicky DJ, Fisher AB, Petersen DR. 2009. Overexpression of peroxiredoxin 6 does not prevent ethanol-mediated oxidative stress and may play a role in hepatic lipid accumulation. J Pharmacol Exp Ther 330: 79–88.
- Roede JR, Stewart BJ, Petersen DR. 2008. Decreased expression of peroxiredoxin 6 in a mouse model of ethanol consumption. Free Radic Biol Med 45: 1551–1558.
- Rokutan K, Kawai K, Asada K. 1987. Inactivation of 2-oxoglutarate dehydrogenase in rat liver mitochondria by its substrate and f-butyl hydroperoxide. J Biochem 101: 415–422.
- Song YH, Liu Q, Lv ZP, Chen YY, Zhou YC, Sun XG. 2008. Protection of a polysaccharide from Salvia miltiorrhiza, a Chinese medicinal herb, against immunological liver injury in mice. Int J Biol Macromol 43: 170–175.
- Vogel SN, Moore RN, Sipe JD, Rosenstreich DL. 1980. BCG-induced enhancement of endotoxin sensitivity in C3H/HeJ mice. J Immunol 124: 2004–2009.
- Wang Y, Feinstein SI, Fisher AB. 2008. Peroxiredoxin 6 as an antioxidant enzyme: protection of lung alveolar epithelial type II cells from H2O2-induced oxidative stress. J Cell Biochem 104: 1274–1285.
- Wang Y, Feinstein SI, Manevich Y, Ho YS, Fisher AB. 2006a. Peroxiredoxin 6 gene-targeted mice show increased lung injury with paraquat-induced oxidative stress. Antioxid Redox Signal 8: 229–237.
- Wang Y, Phelan SA, Manevich Y, Feinstein SI, Fisher AB. 2006b. Transgenic mice overexpressing peroxiredoxin 6 show increased resistance to lung injury in hyperoxia. Am J Respir Cell Mol Biol 10: 481–486.
- Xu JM, Xu SY, Mei Q, Wang DB, Wu JF, Zhang AP. 1997. The model of immunopharmacological liver damage induced by short-rod-shaped bacilli and lipopolysaccharide. Acta Pharmacol Sin 13: 186–188.
Citing Literature
