Follistatin-like 1 is a myokine regulating lipid mobilization during endurance exercise and recovery
Ji Sun Nam
Severance Institute for Vascular and Metabolic Research, Yonsei University College of Medicine, Seoul, Republic of Korea
Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea
Search for more papers by this authorSu-Jeong Park
Graduate School, Yonsei University College of Medicine, Seoul, Republic of Korea
Search for more papers by this authorChul Woo Ahn
Severance Institute for Vascular and Metabolic Research, Yonsei University College of Medicine, Seoul, Republic of Korea
Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea
Search for more papers by this authorEun-Suk Cho
Department of Radiology, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea
Search for more papers by this authorHee-Joo Kim
Department of Health Sciences and Technology, GAIHST, Gachon University, Incheon, Republic of Korea
Search for more papers by this authorCorresponding Author
YuSik Kim
Severance Institute for Vascular and Metabolic Research, Yonsei University College of Medicine, Seoul, Republic of Korea
Correspondence
YuSik Kim, Gangnam Severance Hospital Medical Research Center 237 Dogok-Ro Gangnam-Gu, Seoul 06230, Republic of Korea.
Email: [email protected]
Search for more papers by this authorJi Sun Nam
Severance Institute for Vascular and Metabolic Research, Yonsei University College of Medicine, Seoul, Republic of Korea
Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea
Search for more papers by this authorSu-Jeong Park
Graduate School, Yonsei University College of Medicine, Seoul, Republic of Korea
Search for more papers by this authorChul Woo Ahn
Severance Institute for Vascular and Metabolic Research, Yonsei University College of Medicine, Seoul, Republic of Korea
Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea
Search for more papers by this authorEun-Suk Cho
Department of Radiology, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea
Search for more papers by this authorHee-Joo Kim
Department of Health Sciences and Technology, GAIHST, Gachon University, Incheon, Republic of Korea
Search for more papers by this authorCorresponding Author
YuSik Kim
Severance Institute for Vascular and Metabolic Research, Yonsei University College of Medicine, Seoul, Republic of Korea
Correspondence
YuSik Kim, Gangnam Severance Hospital Medical Research Center 237 Dogok-Ro Gangnam-Gu, Seoul 06230, Republic of Korea.
Email: [email protected]
Search for more papers by this authorJi Sun Nam and Su-Jeong Park contributed equally to this work.
Abstract
Objective
The aim of this study was to investigate the role of the follistatin-like 1 (Fstl1) and disco-interacting protein 2 homolog A (DIP2a) axis in relation to lipid metabolism during and after endurance exercise and to elucidate the mechanisms underlying the metabolic effects of Fstl1 on adipocytes, considering its regulation by exercise and muscle mass and its link to obesity.
Methods
Twenty-nine sedentary males participated in endurance exercise, and blood samples were collected during and after the exercise. Body composition, Fstl1, glycerol, epinephrine, growth hormone, and atrial natriuretic peptide were measured. 3T3-L1 adipocytes, with or without DIP2a knockdown, were treated with Fstl1 to assess glycerol release, cyclic AMP/cyclic GMP production, and hormone sensitive lipase phosphorylation. The association between DIP2a gene expression levels in human adipose tissues and exercise-induced lipolysis was examined.
Results
Fstl1 levels significantly increased during endurance exercise and following recovery, correlating with lean body mass and lipolysis. In 3T3-L1 adipocytes, Fstl1 increased glycerol release, cyclic GMP production, and hormone sensitive lipase activation, but these effects were attenuated by DIP2a knockdown. DIP2a gene expression in human adipose tissues correlated with serum glycerol concentrations during endurance exercise.
Conclusions
Fstl1 is a myokine facilitating lipid mobilization during and after endurance exercise through DIP2a-mediated lipolytic effects in adipocytes.
CONFLICT OF INTEREST STATEMENT
The authors declared no conflict of interest.
Supporting Information
| Filename | Description |
|---|---|
| oby23949-sup-0001-FigureS1.tifTIFF image, 480.5 KB | Figure S1. Schematic representation of endurance exercise experiment. Subject engaged in endurance exercise on a treadmill, expending 300 calories at 70% of their age-predicted HRmax. Standard meals (549 Kcal) were given 4 hours before the exercise; fat biopsies were taken 7–10 days prior to the experiment. Blood samples were taken at indicated time points: baseline, 1/2Ex, halfway mark of exercise; P0, immediately after the exercise; P30, 30 min after the exercise; P60, 60 min after the exercise; P120, 120 min after the exercise. |
| oby23949-sup-0002-Tables.docxWord 2007 document , 25.8 KB | Table S1. Demographic characteristics of subjects. Table S2. List of primers used in the study. Table S3. Quantitative measurements of band intensities of Figure 2D. Table S4. Quantitative measurements of band intensities of Figure 2E. Table S5. Quantitative measurements of band intensities of Figure 2F. Table S6. Quantitative measurements of band intensities of Figure 5C. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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