We recently developed an animal model to investigate the effects of eccentric contraction (ECC) and blood flow restriction (BFR) on muscle tissue at the cellular level. This study clarified the effects of repeated BFR, ECC, and BFR combined with ECC (BFR+ECC) on muscle fiber hypertrophy.

Methods

Male Wistar rats were assigned to 3 groups: BFR, ECC, and BFR+ECC. The contralateral leg in the BFR group served as a control (CONT). Muscle fiber cross-sectional area (CSA) of the tibialis anterior was determined after the respective treatments for 6 weeks.

Results

CSA was greater in the BFR+ECC group than in the CONT (P < 0.01) and ECC (P < 0.05) groups. CSA was greater in the BFR group than that in the CONT group (P < 0.05).

Cnclusions

These results suggest that repeated BFR alone as well as BFR+ECC induces muscle fiber hypertrophy at the cellular level. Muscle Nerve 55: 274–276, 2017

REFERENCES

1 Laurentino GC, Ugrinowitsch C, Roschel H, Aoki MS, Soares AG, Neves M Jr, et al. Strength training with blood flow restriction diminishes myostatin gene expression. Med Sci Sports Exerc 2012; 44: 406–412.
10.1249/MSS.0b013e318233b4bc
CAS PubMed Web of Science® Google Scholar
2 Lixandrao ME, Ugrinowitsch C, Laurentino G, Libardi CA, Aihara AY, Cardoso FN, et al. Effects of exercise intensity and occlusion pressure after 12 weeks of resistance training with blood-flow restriction. Eur J Appl Physiol 2015; 115: 2471–2480.
10.1007/s00421-015-3253-2
PubMed Web of Science® Google Scholar
3 Martin-Hernandez J, Marin PJ, Menendez H, Ferrero C, Loenneke JP, Herrero AJ. Muscular adaptations after two different volumes of blood flow-restricted training. Scand J Med Sci Sports 2013; 23: e114–e120.
10.1111/sms.12036
CAS PubMed Web of Science® Google Scholar
4 Vechin FC, Libardi CA, Conceicao MS, Damas FR, Lixandrao ME, Berton RP, et al. Comparisons between low-intensity resistance training with blood flow restriction and high-intensity resistance training on quadriceps muscle mass and strength in elderly. J Strength Cond Res 2015; 29: 1071–1076.
10.1519/JSC.0000000000000703
PubMed Web of Science® Google Scholar
5 Yasuda T, Loenneke JP, Thiebaud RS, Abe T. Effects of blood flow restricted low-intensity concentric or eccentric training on muscle size and strength. PLoS One 2012; 7: e52843.
10.1371/journal.pone.0052843
CAS PubMed Web of Science® Google Scholar
6 Cook SB, Brown KA, Deruisseau K, Kanaley JA, Ploutz-Snyder LL. Skeletal muscle adaptations following blood flow-restricted training during 30 days of muscular unloading. J Appl Physiol 2010; 109: 341–349.
10.1152/japplphysiol.01288.2009
PubMed Web of Science® Google Scholar
7 Cook SB, Kanaley JA, Ploutz-Snyder LL. Neuromuscular function following muscular unloading and blood flow restricted exercise. Eur J Appl Physiol 2014; 114: 1357–1365.
10.1007/s00421-014-2864-3
PubMed Web of Science® Google Scholar
8 Kubota A, Sakuraba K, Koh S, Ogura Y, Tamura Y. Blood flow restriction by low compressive force prevents disuse muscular weakness. J Sci Med Sport 2011; 14: 95–99.
10.1016/j.jsams.2010.08.007
PubMed Web of Science® Google Scholar
9 Kubota A, Sakuraba K, Sawaki K, Sumide T, Tamura Y. Prevention of disuse muscular weakness by restriction of blood flow. Med Sci Sports Exerc 2008; 40: 529–534.
10.1249/MSS.0b013e31815ddac6
PubMed Web of Science® Google Scholar
10 Takarada Y, Takazawa H, Ishii N. Applications of vascular occlusion diminish disuse atrophy of knee extensor muscles. Med Sci Sports Exerc 2000; 32: 2035–2039.
10.1097/00005768-200012000-00011
CAS PubMed Web of Science® Google Scholar
11 Loenneke JP, Abe T, Wilson JM, Thiebaud RS, Fahs CA, Rossow LM, et al. Blood flow restriction: an evidence based progressive model (Review). Acta Physiol Hung 2012; 99: 235–250.
10.1556/APhysiol.99.2012.3.1
CAS PubMed Web of Science® Google Scholar
12 Scott BR, Loenneke JP, Slattery KM, Dascombe BJ. Exercise with blood flow restriction: an updated evidence-based approach for enhanced muscular development. Sports Med 2015; 45: 313–325.
10.1007/s40279-014-0288-1
PubMed Web of Science® Google Scholar
13 Sudo M, Ando S, Poole DC, Kano Y. Blood flow restriction prevents muscle damage but not protein synthesis signaling following eccentric contractions. Physiol Rep 2015; 3:pii: e12449.
10.14814/phy2.12449
CAS PubMed Web of Science® Google Scholar
14 Kano Y, Masuda K, Furukawa H, Sudo M, Mito K, Sakamoto K. Histological skeletal muscle damage and surface EMG relationships following eccentric contractions. J Physiol Sci 2008; 58: 349–355.
10.2170/physiolsci.RP004908
PubMed Web of Science® Google Scholar
15 Sudo M, Kano Y. Myofiber apoptosis occurs in the inflammation and regeneration phase following eccentric contractions in rats. J Physiol Sci 2009; 59: 405–412.
10.1007/s12576-009-0049-3
CAS PubMed Web of Science® Google Scholar
16 Duncan ND, Williams DA, Lynch GS. Adaptations in rat skeletal muscle following long-term resistance exercise training. Eur J Appl Physiol Occup Physiol 1998; 77: 372–378.
10.1007/s004210050347
CAS PubMed Web of Science® Google Scholar
17 Ochi E, Nakazato K, Ishii N. Muscular hypertrophy and changes in cytokine production after eccentric training in the rat skeletal muscle. J Strength Cond Res 2011; 25: 2283–2292.
10.1519/JSC.0b013e3181f1592e
PubMed Web of Science® Google Scholar
18 Tanaka M, Hirayama Y, Fujita N, Fujino H. Electrical stimulation using sine waveform prevents unloading-induced muscle atrophy in the deep calf muscles of rat. Acta Histochem 2014; 116: 1192–1198.
10.1016/j.acthis.2014.06.009
PubMed Web of Science® Google Scholar
19 Nakajima T, Yasuda T, Koide S, Yamasoba T, Obi S, Toyoda S, et al. Repetitive restriction of muscle blood flow enhances mTOR signaling pathways in a rat model. Heart Vessels 2016; 31: 1685–1695.
10.1007/s00380-016-0801-6
PubMed Web of Science® Google Scholar
20 Loenneke JP1, Fahs CA, Rossow LM, Abe T, Bemben MG. The anabolic benefits of venous blood flow restriction training may be induced by muscle cell swelling. Med Hypotheses 2012; 78: 151–154.
10.1016/j.mehy.2011.10.014
CAS PubMed Web of Science® Google Scholar
21 Spranger MD, Krishnan AC, Levy PD, O'Leary DS, Smith SA. Blood flow restriction training and the exercise pressor reflex: a call for concern. Am J Physiol Heart Circ Physiol 2015; 309: H1440–H1452.
10.1152/ajpheart.00208.2015
CAS PubMed Web of Science® Google Scholar

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Volume55, Issue2

February 2017

Pages 274-276

Repeated blood flow restriction induces muscle fiber hypertrophy

ABSTRACT

Introduction

Methods

Results

Cnclusions

REFERENCES

Citing Literature

References

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Repeated blood flow restriction induces muscle fiber hypertrophy

ABSTRACT

Introduction

Methods

Results

Cnclusions

REFERENCES

Citing Literature

References

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