Chronic peripheral nerve compression disrupts paranodal axoglial junctions
Yoshinori Otani PhD
Department of Neuroscience, Cell Biology, and Physiology, Boonshoft School of Medicine, Wright State University, 3640 Colonel Glenn Highway, Dayton, Ohio, 45435 USA
Search for more papers by this authorLeonid M. Yermakov BS
Department of Neuroscience, Cell Biology, and Physiology, Boonshoft School of Medicine, Wright State University, 3640 Colonel Glenn Highway, Dayton, Ohio, 45435 USA
Search for more papers by this authorJeffrey L. Dupree PhD
Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, Virginia, USA
Search for more papers by this authorCorresponding Author
Keiichiro Susuki MD, PhD
Department of Neuroscience, Cell Biology, and Physiology, Boonshoft School of Medicine, Wright State University, 3640 Colonel Glenn Highway, Dayton, Ohio, 45435 USA
Correspondence to: K. Susuki; e-mail: [email protected]Search for more papers by this authorYoshinori Otani PhD
Department of Neuroscience, Cell Biology, and Physiology, Boonshoft School of Medicine, Wright State University, 3640 Colonel Glenn Highway, Dayton, Ohio, 45435 USA
Search for more papers by this authorLeonid M. Yermakov BS
Department of Neuroscience, Cell Biology, and Physiology, Boonshoft School of Medicine, Wright State University, 3640 Colonel Glenn Highway, Dayton, Ohio, 45435 USA
Search for more papers by this authorJeffrey L. Dupree PhD
Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, Virginia, USA
Search for more papers by this authorCorresponding Author
Keiichiro Susuki MD, PhD
Department of Neuroscience, Cell Biology, and Physiology, Boonshoft School of Medicine, Wright State University, 3640 Colonel Glenn Highway, Dayton, Ohio, 45435 USA
Correspondence to: K. Susuki; e-mail: [email protected]Search for more papers by this authorFunding: This work was supported by Veterans Affairs Merit Grant (5IO1BX002565 to J.L.D.) and National Institutes of Health (NIH) -National Institute of Neurological Disorders and Stroke Center Core Grant 5P30 NS047463.
Conflicts of Interest: The authors declare no competing financial interests.
ABSTRACT
Introduction
Peripheral nerves are often exposed to mechanical stress leading to compression neuropathies. The pathophysiology underlying nerve dysfunction by chronic compression is largely unknown.
Methods
We analyzed molecular organization and fine structures at and near nodes of Ranvier in a compression neuropathy model in which a silastic tube was placed around the mouse sciatic nerve.
Results
Immunofluorescence study showed that clusters of cell adhesion complex forming paranodal axoglial junctions were dispersed and overlapped frequently with juxtaparanodal components. These paranodal changes occurred without internodal myelin damage. The distribution and pattern of paranodal disruption suggests that these changes are the direct result of mechanical stress. Electron microscopy confirmed loss of paranodal axoglial junctions.
Conclusions
Our data show that chronic nerve compression disrupts paranodal junctions and axonal domains required for proper peripheral nerve function. These results provide important clues toward better understanding of the pathophysiology underlying nerve dysfunction in compression neuropathies. Muscle Nerve 55: 544–554, 2017
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