CCR9 AND CCR7 are overexpressed in CD4–CD8– thymocytes of myasthenia gravis patients
Qianru Li PhD
Department of Immunology, Basic Medical College, Zhengzhou University, No.100 of Science Road, Zhengzhou 450001, Henan Province, PR China
Search for more papers by this authorPingping Liu PhD
Department of Immunology, Basic Medical College, Zhengzhou University, No.100 of Science Road, Zhengzhou 450001, Henan Province, PR China
Search for more papers by this authorXiaoyan Xuan PhD
Department of Immunology, Basic Medical College, Zhengzhou University, No.100 of Science Road, Zhengzhou 450001, Henan Province, PR China
Search for more papers by this authorJunfeng Zhang MD
Department of Laboratory, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, PR China
Search for more papers by this authorYun Zhang MD
Department of Immunology, Basic Medical College, Zhengzhou University, No.100 of Science Road, Zhengzhou 450001, Henan Province, PR China
Search for more papers by this authorZhengkun Zhu MD
Department of Immunology, Basic Medical College, Zhengzhou University, No.100 of Science Road, Zhengzhou 450001, Henan Province, PR China
Search for more papers by this authorFeng Gao MD
Henan Institute of Medical Sciences, Zhengzhou University, Zhengzhou, Henan Province, PR China
Search for more papers by this authorQingyong Zhang MD
The Second Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, PR China
Search for more papers by this authorCorresponding Author
Ying Du PhD
Department of Immunology, Basic Medical College, Zhengzhou University, No.100 of Science Road, Zhengzhou 450001, Henan Province, PR China
Correspondence to: Y. Du, e-mail: [email protected]Search for more papers by this authorQianru Li PhD
Department of Immunology, Basic Medical College, Zhengzhou University, No.100 of Science Road, Zhengzhou 450001, Henan Province, PR China
Search for more papers by this authorPingping Liu PhD
Department of Immunology, Basic Medical College, Zhengzhou University, No.100 of Science Road, Zhengzhou 450001, Henan Province, PR China
Search for more papers by this authorXiaoyan Xuan PhD
Department of Immunology, Basic Medical College, Zhengzhou University, No.100 of Science Road, Zhengzhou 450001, Henan Province, PR China
Search for more papers by this authorJunfeng Zhang MD
Department of Laboratory, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, PR China
Search for more papers by this authorYun Zhang MD
Department of Immunology, Basic Medical College, Zhengzhou University, No.100 of Science Road, Zhengzhou 450001, Henan Province, PR China
Search for more papers by this authorZhengkun Zhu MD
Department of Immunology, Basic Medical College, Zhengzhou University, No.100 of Science Road, Zhengzhou 450001, Henan Province, PR China
Search for more papers by this authorFeng Gao MD
Henan Institute of Medical Sciences, Zhengzhou University, Zhengzhou, Henan Province, PR China
Search for more papers by this authorQingyong Zhang MD
The Second Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, PR China
Search for more papers by this authorCorresponding Author
Ying Du PhD
Department of Immunology, Basic Medical College, Zhengzhou University, No.100 of Science Road, Zhengzhou 450001, Henan Province, PR China
Correspondence to: Y. Du, e-mail: [email protected]Search for more papers by this authorThis work was supported by a grant from the National Natural Science Foundation of China (81172874).
ABSTRACT
Introduction: Chemokine CC motif receptors 9 and 7 (CCR9 and CCR7) play a major role in the migration of T-cell precursors to the thymus to initiate T thymopoiesis. However, their role in development of T-cells in myasthenia gravis (MG) patients has not been fully elucidated. Methods: Expression and distribution of CCR9+ and CCR7+ cells were detected by flow cytometry and immunofluorescence. Real-time polymerase chain reaction was used to check the adhesion molecules on CD4–CD8– double-negative (DN) thymocytes. Results: CCR9 and CCR7 expression by DN thymocytes increased in the MG thymus; the levels of CCR9, CCR7, interleukin-7R mRNA increased, and CXCR4 levels decreased compared with levels in the non-MG thymus. More CCR7 and CCR9 double-positive (DP) thymocytes were gathered near the subcapsular region in MG thymus. Conclusions: Enhanced expression of CCR9 and CCR7 may complicate the differentiation of DP thymocytes from the DN stage in MG thymus. Muscle Nerve, 2016 Muscle Nerve 55: 84–90, 2017
Supporting Information
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