J-difference-edited MRS measures of γ-aminobutyric acid before and after acute caffeine administration
Corresponding Author
Georg Oeltzschner
Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, Maryland
F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, Maryland
*Georg Oeltzschner and Helge J. Zöllner contributed equally to this work.
Correspondence Georg Oeltzschner, N 600 Wolfe Street, Park 359, Baltimore, MD 21287. Email: [email protected]Search for more papers by this authorHelge J. Zöllner
Institute for Clinical Neuroscience and Medical Psychology, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany
Department of Diagnostic and Interventional Radiology, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany
*Georg Oeltzschner and Helge J. Zöllner contributed equally to this work.
Search for more papers by this authorMarc Jonuscheit
Department of Diagnostic and Interventional Radiology, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany
Search for more papers by this authorRotem S. Lanzman
Department of Diagnostic and Interventional Radiology, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany
Search for more papers by this authorAlfons Schnitzler
Institute for Clinical Neuroscience and Medical Psychology, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany
Search for more papers by this authorHans-Jörg Wittsack
Department of Diagnostic and Interventional Radiology, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany
Search for more papers by this authorCorresponding Author
Georg Oeltzschner
Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, Maryland
F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, Maryland
*Georg Oeltzschner and Helge J. Zöllner contributed equally to this work.
Correspondence Georg Oeltzschner, N 600 Wolfe Street, Park 359, Baltimore, MD 21287. Email: [email protected]Search for more papers by this authorHelge J. Zöllner
Institute for Clinical Neuroscience and Medical Psychology, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany
Department of Diagnostic and Interventional Radiology, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany
*Georg Oeltzschner and Helge J. Zöllner contributed equally to this work.
Search for more papers by this authorMarc Jonuscheit
Department of Diagnostic and Interventional Radiology, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany
Search for more papers by this authorRotem S. Lanzman
Department of Diagnostic and Interventional Radiology, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany
Search for more papers by this authorAlfons Schnitzler
Institute for Clinical Neuroscience and Medical Psychology, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany
Search for more papers by this authorHans-Jörg Wittsack
Department of Diagnostic and Interventional Radiology, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany
Search for more papers by this authorFunding information: This work has been supported by the Sonderforschungsbereich 974 (SFB 974) of the German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) and used tools developed under NIH R01EB016089, R01EB023963, and P41EB015909. GO also received salary support from NIH R01MH106564 and R21MH098228
Abstract
Purpose
The aim of this study was to investigate potential effects of acute caffeine intake on J-difference-edited MRS measures of the primary inhibitory neurotransmitter γ-aminobutyric acid (GABA).
Methods
J-difference-edited Mescher-Garwood PRESS (MEGA-PRESS) and conventional PRESS data were acquired at 3T from voxels in the anterior cingulate and occipital area of the brain in 15 healthy subjects, before and after oral intake of a 200-mg caffeine dose. MEGA-PRESS data were analyzed with the MATLAB-based Gannet tool to estimate GABA+ macromolecule (GABA+) levels, while PRESS data were analyzed with LCModel to estimate levels of glutamate, glutamate+glutamine, N-acetylaspartate, and myo-inositol. All metabolites were quantified with respect to the internal reference compounds creatine and tissue water, and compared between the pre- and post-caffeine intake condition.
Results
For both MRS voxels, mean GABA+ estimates did not differ before and after caffeine intake. Slightly lower estimates of myo-inositol were observed after caffeine intake in both voxels. N-acetylaspartate, glutamate, and glutamate+glutamine did not show significant differences between conditions.
Conclusion
Mean GABA+ estimates from J-difference-edited MRS in two different brain regions are not altered by acute oral administration of caffeine. These findings may increase subject recruitment efficiency for MRS studies.
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