Use of magnetic nanoparticles to monitor alginate-encapsulated βTC-tet cells
Ioannis Constantinidis
Department of Medicine, Division of Endocrinology, University of Florida College of Medicine, Gainesville, Florida, USA
National High Magnetic Field Laboratory, Tallahassee, Florida, USA
Search for more papers by this authorSamuel C. Grant
National High Magnetic Field Laboratory, Tallahassee, Florida, USA
Department of Neuroscience, University of Florida McKnight Brain Institute, Gainesville, Florida, USA
Department of Chemical and Biomedical Engineering, Florida State University, Tallahassee, Florida, USA
Search for more papers by this authorCorresponding Author
Nicholas E. Simpson
Department of Medicine, Division of Endocrinology, University of Florida College of Medicine, Gainesville, Florida, USA
Department of Medicine, Division of Endocrinology, University of Florida, 1600 SW Archer Rd., PO Box 100226, Gainesville, FL 32610-0226===Search for more papers by this authorJose A. Oca-Cossio
Department of Medicine, Division of Endocrinology, University of Florida College of Medicine, Gainesville, Florida, USA
Search for more papers by this authorCarol A. Sweeney
Department of Medicine, Division of Endocrinology, University of Florida College of Medicine, Gainesville, Florida, USA
Search for more papers by this authorHui Mao
Department of Radiology, Emory University School of Medicine, Atlanta, Georgia, USA
Search for more papers by this authorStephen J. Blackband
National High Magnetic Field Laboratory, Tallahassee, Florida, USA
Department of Neuroscience, University of Florida McKnight Brain Institute, Gainesville, Florida, USA
Search for more papers by this authorAthanassios Sambanis
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA
Search for more papers by this authorIoannis Constantinidis
Department of Medicine, Division of Endocrinology, University of Florida College of Medicine, Gainesville, Florida, USA
National High Magnetic Field Laboratory, Tallahassee, Florida, USA
Search for more papers by this authorSamuel C. Grant
National High Magnetic Field Laboratory, Tallahassee, Florida, USA
Department of Neuroscience, University of Florida McKnight Brain Institute, Gainesville, Florida, USA
Department of Chemical and Biomedical Engineering, Florida State University, Tallahassee, Florida, USA
Search for more papers by this authorCorresponding Author
Nicholas E. Simpson
Department of Medicine, Division of Endocrinology, University of Florida College of Medicine, Gainesville, Florida, USA
Department of Medicine, Division of Endocrinology, University of Florida, 1600 SW Archer Rd., PO Box 100226, Gainesville, FL 32610-0226===Search for more papers by this authorJose A. Oca-Cossio
Department of Medicine, Division of Endocrinology, University of Florida College of Medicine, Gainesville, Florida, USA
Search for more papers by this authorCarol A. Sweeney
Department of Medicine, Division of Endocrinology, University of Florida College of Medicine, Gainesville, Florida, USA
Search for more papers by this authorHui Mao
Department of Radiology, Emory University School of Medicine, Atlanta, Georgia, USA
Search for more papers by this authorStephen J. Blackband
National High Magnetic Field Laboratory, Tallahassee, Florida, USA
Department of Neuroscience, University of Florida McKnight Brain Institute, Gainesville, Florida, USA
Search for more papers by this authorAthanassios Sambanis
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA
Search for more papers by this authorAbstract
Noninvasive monitoring of tissue-engineered constructs is an important component in optimizing construct design and assessing therapeutic efficacy. In recent years, cellular and molecular imaging initiatives have spurred the use of iron oxide-based contrast agents in the field of NMR imaging. Although their use in medical research has been widespread, their application in tissue engineering has been limited. In this study, the utility of monocrystalline iron oxide nanoparticles (MIONs) as an NMR contrast agent was evaluated for βTC-tet cells encapsulated within alginate/poly-L-lysine/alginate (APA) microbeads. The constructs were labeled with MIONs in two different ways: 1) MION-labeled βTC-tet cells were encapsulated in APA beads (i.e., intracellular compartment), and 2) MION particles were suspended in the alginate solution prior to encapsulation so that the alginate matrix was labeled with MIONs instead of the cells (i.e., extracellular compartment). The data show that although the location of cells can be identified within APA beads, cell growth or rearrangement within these constructs cannot be effectively monitored, regardless of the location of MION compartmentalization. The advantages and disadvantages of these techniques and their potential use in tissue engineering are discussed. Magn Reson Med 61:282–290, 2009. © 2009 Wiley-Liss, Inc.
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