Sulfated Glycopolymer Thin Films—Preparation, Characterization, and Biological Activity
Ringo Grombe
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Search for more papers by this authorMarie F. Gouzy
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Search for more papers by this authorManfred F. Maitz
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Search for more papers by this authorUwe Freundenberg
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Search for more papers by this authorStefan Zschoche
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Search for more papers by this authorFrank Simon
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Search for more papers by this authorTilo Pompe
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Search for more papers by this authorClaudia Sperling
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Search for more papers by this authorCorresponding Author
Carsten Werner
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Institute of Biomaterials and Biomedical Engineering, University of Toronto, 5, King's College Road, M5S 3G8 Toronto, Ontario, Canada
Institute of Biomaterials and Biomedical Engineering, University of Toronto, 5, King's College Road, M5S 3G8 Toronto, Ontario, Canada. Fax: (+1) 351 4658 533Search for more papers by this authorRingo Grombe
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Search for more papers by this authorMarie F. Gouzy
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Search for more papers by this authorManfred F. Maitz
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Search for more papers by this authorUwe Freundenberg
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Search for more papers by this authorStefan Zschoche
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Search for more papers by this authorFrank Simon
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Search for more papers by this authorTilo Pompe
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Search for more papers by this authorClaudia Sperling
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Search for more papers by this authorCorresponding Author
Carsten Werner
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany
Institute of Biomaterials and Biomedical Engineering, University of Toronto, 5, King's College Road, M5S 3G8 Toronto, Ontario, Canada
Institute of Biomaterials and Biomedical Engineering, University of Toronto, 5, King's College Road, M5S 3G8 Toronto, Ontario, Canada. Fax: (+1) 351 4658 533Search for more papers by this authorAbstract
The impact of heparinoid characteristics on model surfaces obtained from immobilization of sole sulfate groups as well as sulfated glycosides, sulfated cellulose, and definite heparin has been investigated. The obtained layers were physico-chemically characterized regarding film thickness, chemical composition, wettability, and surface morphology. Antithrombin adsorption, studied by fluorescence labeling, revealed a strong dependence on the presence of glycosidic structures and on the molecular weight of the grafted saccharide. On contact with whole blood, the coatings resulted in a diminished plasmatic and cellular coagulation in vitro, which did not reflect well the antithrombin binding. Therefore, more complex activating pathways are discussed.
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