A new path in defining light parameters for hair growth: Discovery and modulation of photoreceptors in human hair follicle
Serena Buscone BSc
Faculty of Life Sciences, University of Bradford, Centre for Skin Sciences, Bradford, West Yorkshire BD7 1DP, United Kingdom
Philips Research, High Tech Campus 34, Eindhoven 5656 AE, The Netherlands
Search for more papers by this authorAndrei N. Mardaryev MD, PhD
Faculty of Life Sciences, University of Bradford, Centre for Skin Sciences, Bradford, West Yorkshire BD7 1DP, United Kingdom
Search for more papers by this authorBianca Raafs BSc
Philips Research, High Tech Campus 34, Eindhoven 5656 AE, The Netherlands
Search for more papers by this authorJan W. Bikker
Consultants in Quantitative Methods BV, Eindhoven, The Netherlands
Search for more papers by this authorCarsten Sticht PhD
Faculty Mannheim, University of Heidelberg, Center of Medical Research, Heidelberg, Germany
Search for more papers by this authorNorbert Gretz MD, PhD
Faculty Mannheim, University of Heidelberg, Center of Medical Research, Heidelberg, Germany
Search for more papers by this authorNilofer Farjo MD
Farjo Hair Institute, Manchester, United Kingdom
Search for more papers by this authorCorresponding Author
Natallia E. Uzunbajakava PhD
Philips Research, High Tech Campus 34, Eindhoven 5656 AE, The Netherlands
Correspondence to: Dr. Natalia V. Botchkareva, MD, University of Bradford, Centre for Skin Sciences, Richmond Road, Bradford, West Yorkshire BD7 1DP, England. E-mail: [email protected]
Correspondence to: Dr. ir. Natallia E. Uzunbajakava, MD, PhD, Philips Research, High Tech Campus 34, 5656 AE, Eindhoven, The Netherlands. E-mail: [email protected]
Search for more papers by this authorCorresponding Author
Natalia V. Botchkareva MD, PhD
Faculty of Life Sciences, University of Bradford, Centre for Skin Sciences, Bradford, West Yorkshire BD7 1DP, United Kingdom
Correspondence to: Dr. Natalia V. Botchkareva, MD, University of Bradford, Centre for Skin Sciences, Richmond Road, Bradford, West Yorkshire BD7 1DP, England. E-mail: [email protected]
Correspondence to: Dr. ir. Natallia E. Uzunbajakava, MD, PhD, Philips Research, High Tech Campus 34, 5656 AE, Eindhoven, The Netherlands. E-mail: [email protected]
Search for more papers by this authorSerena Buscone BSc
Faculty of Life Sciences, University of Bradford, Centre for Skin Sciences, Bradford, West Yorkshire BD7 1DP, United Kingdom
Philips Research, High Tech Campus 34, Eindhoven 5656 AE, The Netherlands
Search for more papers by this authorAndrei N. Mardaryev MD, PhD
Faculty of Life Sciences, University of Bradford, Centre for Skin Sciences, Bradford, West Yorkshire BD7 1DP, United Kingdom
Search for more papers by this authorBianca Raafs BSc
Philips Research, High Tech Campus 34, Eindhoven 5656 AE, The Netherlands
Search for more papers by this authorJan W. Bikker
Consultants in Quantitative Methods BV, Eindhoven, The Netherlands
Search for more papers by this authorCarsten Sticht PhD
Faculty Mannheim, University of Heidelberg, Center of Medical Research, Heidelberg, Germany
Search for more papers by this authorNorbert Gretz MD, PhD
Faculty Mannheim, University of Heidelberg, Center of Medical Research, Heidelberg, Germany
Search for more papers by this authorNilofer Farjo MD
Farjo Hair Institute, Manchester, United Kingdom
Search for more papers by this authorCorresponding Author
Natallia E. Uzunbajakava PhD
Philips Research, High Tech Campus 34, Eindhoven 5656 AE, The Netherlands
Correspondence to: Dr. Natalia V. Botchkareva, MD, University of Bradford, Centre for Skin Sciences, Richmond Road, Bradford, West Yorkshire BD7 1DP, England. E-mail: [email protected]
Correspondence to: Dr. ir. Natallia E. Uzunbajakava, MD, PhD, Philips Research, High Tech Campus 34, 5656 AE, Eindhoven, The Netherlands. E-mail: [email protected]
Search for more papers by this authorCorresponding Author
Natalia V. Botchkareva MD, PhD
Faculty of Life Sciences, University of Bradford, Centre for Skin Sciences, Bradford, West Yorkshire BD7 1DP, United Kingdom
Correspondence to: Dr. Natalia V. Botchkareva, MD, University of Bradford, Centre for Skin Sciences, Richmond Road, Bradford, West Yorkshire BD7 1DP, England. E-mail: [email protected]
Correspondence to: Dr. ir. Natallia E. Uzunbajakava, MD, PhD, Philips Research, High Tech Campus 34, 5656 AE, Eindhoven, The Netherlands. E-mail: [email protected]
Search for more papers by this authorAbstract
Background and Objective
Though devices for hair growth based on low levels of light have shown encouraging results, further improvements of their efficacy is impeded by a lack of knowledge on the exact molecular targets that mediate physiological response in skin and hair follicle. The aim of this study was to investigate the expression of selected light-sensitive receptors in the human hair follicle and to study the impact of UV-free blue light on hair growth ex vivo.
Material and Methods
The expression of Opsin receptors in human skin and hair follicles has been characterized using RT-qPCR and immunofluorescence approaches. The functional significance of Opsin 3 was assessed by silencing its expression in the hair follicle cells followed by a transcriptomic profiling. Proprietary LED-based devices emitting two discrete visible wavelengths were used to access the effects of selected optical parameters on hair growth ex vivo and outer root sheath cells in vitro.
Results
The expression of OPN2 (Rhodopsin) and OPN3 (Panopsin, Encephalopsin) was detected in the distinct compartments of skin and anagen hair follicle. Treatment with 3.2 J/cm2 of blue light with 453 nm central wavelength significantly prolonged anagen phase in hair follicles ex vivo that was correlated with sustained proliferation in the light-treated samples. In contrast, hair follicle treatment with 3.2 J/cm2 of 689 nm light (red light) did not significantly affect hair growth ex vivo. Silencing of OPN3 in the hair follicle outer root sheath cells resulted in the altered expression of genes involved in the control of proliferation and apoptosis, and abrogated stimulatory effects of blue light (3.2 J/cm2; 453 nm) on proliferation in the outer root sheath cells.
Conclusions
We provide the first evidence that (i) OPN2 and OPN3 are expressed in human hair follicle, and (ii) A 453 nm blue light at low radiant exposure exerts a positive effect on hair growth ex vivo, potentially via interaction with OPN3. Lasers Surg. Med. 49:705–718, 2017. © 2017 Wiley Periodicals, Inc.
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