The sensitivity of glioma cells to pyropheophorbide-αmethyl ester-mediated photodynamic therapy is enhanced by inhibiting ABCG2
Li Pan MSc
Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016 P.R. China
Search for more papers by this authorHaidan Lin MSc
Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016 P.R. China
Search for more papers by this authorSi Tian MSc
Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016 P.R. China
Search for more papers by this authorCorresponding Author
Dingqun Bai PhD
Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016 P.R. China
Correspondence to: Dingqun Bai, PhD and Yuhan Kong, PhD, Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China. E-mail: [email protected] (DB); [email protected] (YK)
Search for more papers by this authorCorresponding Author
Yuhan Kong PhD
Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016 P.R. China
Correspondence to: Dingqun Bai, PhD and Yuhan Kong, PhD, Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China. E-mail: [email protected] (DB); [email protected] (YK)
Search for more papers by this authorLehua Yu PhD
Department of Rehabilitation Medicine, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010 P.R. China
Search for more papers by this authorLi Pan MSc
Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016 P.R. China
Search for more papers by this authorHaidan Lin MSc
Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016 P.R. China
Search for more papers by this authorSi Tian MSc
Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016 P.R. China
Search for more papers by this authorCorresponding Author
Dingqun Bai PhD
Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016 P.R. China
Correspondence to: Dingqun Bai, PhD and Yuhan Kong, PhD, Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China. E-mail: [email protected] (DB); [email protected] (YK)
Search for more papers by this authorCorresponding Author
Yuhan Kong PhD
Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016 P.R. China
Correspondence to: Dingqun Bai, PhD and Yuhan Kong, PhD, Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China. E-mail: [email protected] (DB); [email protected] (YK)
Search for more papers by this authorLehua Yu PhD
Department of Rehabilitation Medicine, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010 P.R. China
Search for more papers by this authorAbstract
Background and Objective
To study the mechanisms of human glioblastoma cell resistance to methyl ester pyropheophorbide-a-mediated photodynamic therapy (MPPa-PDT) and the relationship between the cells and adenosine triphosphate-binding cassette superfamily G member 2 (ABCG2).
Study Design/Materials and Methods
The sensitivity of four human glioma cell lines (U87, A172, SHG-44, and U251) to MPPa-PDT was detected with a CCK-8 assay. Cell apoptosis, intracellular MPPa, and singlet oxygen were tested with flow cytometry. The mRNA and protein expression of ATP-binding cassette transporters (ABCG2, MRP1, and MDR1) were detected by PCR and Western blot, respectively.
Results
Both the sensitivity to MPPa-PDT and intracellular MPPa in A172 were the lowest among the four cell lines, while expression of ABCG2 mRNA and protein in A172 were the highest. The intracellular MPPa and ROS in A172 receiving MPPa-PDT significantly increased after using the ABCG2 inhibitor fumitremorgin C (FTC). Both cell viability and apoptosis in A172 cells undergoing MPPa-PDT were significantly improved with FTC.
Conclusions
ABCG2 plays a significant role in the resistance of A172 to MPPa-PDT. Lasers Surg. Med. 49:719–726, 2017. © 2017 Wiley Periodicals, Inc.
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