Impact of suburban cropland intensification and afforestation on microbial biodiversity and C sequestration in paddy soils
Youlin Luo
College of Resources, Sichuan Agricultural University, Chengdu, China
Search for more papers by this authorJie Shen
College of Resources, Sichuan Agricultural University, Chengdu, China
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China
Search for more papers by this authorYuehan Dou
Department of Health and Environmental Sciences, School of Sciences, Xi'an Jiaotong-Liverpool University, Suzhou, China
Search for more papers by this authorLingke Guo
College of Resources, Sichuan Agricultural University, Chengdu, China
Search for more papers by this authorZhiyuan Xin
College of Resources and Environment Engineering, Mianyang Normal University, Mianyang, China
Search for more papers by this authorQi Tao
College of Resources, Sichuan Agricultural University, Chengdu, China
Search for more papers by this authorQiquan Li
College of Resources, Sichuan Agricultural University, Chengdu, China
Key Laboratory of Investigation and Monitoring, Protection and Utilization for Cultivated Land Resources, Ministry of Natural Resources, Chengdu, China
Search for more papers by this authorBing Li
College of Resources, Sichuan Agricultural University, Chengdu, China
Key Laboratory of Investigation and Monitoring, Protection and Utilization for Cultivated Land Resources, Ministry of Natural Resources, Chengdu, China
Search for more papers by this authorRong Huang
College of Resources, Sichuan Agricultural University, Chengdu, China
Key Laboratory of Investigation and Monitoring, Protection and Utilization for Cultivated Land Resources, Ministry of Natural Resources, Chengdu, China
Search for more papers by this authorQiang Xu
College of Resources, Sichuan Agricultural University, Chengdu, China
Search for more papers by this authorHuanxiu Li
Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, China
Search for more papers by this authorCorresponding Author
Changquan Wang
College of Resources, Sichuan Agricultural University, Chengdu, China
Key Laboratory of Investigation and Monitoring, Protection and Utilization for Cultivated Land Resources, Ministry of Natural Resources, Chengdu, China
Correspondence
Changquan Wang, Number 211, Huimin Road, Wenjiang District, Chengdu City, Sichuan Province, 611130, China.
Email: [email protected]
Search for more papers by this authorYoulin Luo
College of Resources, Sichuan Agricultural University, Chengdu, China
Search for more papers by this authorJie Shen
College of Resources, Sichuan Agricultural University, Chengdu, China
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China
Search for more papers by this authorYuehan Dou
Department of Health and Environmental Sciences, School of Sciences, Xi'an Jiaotong-Liverpool University, Suzhou, China
Search for more papers by this authorLingke Guo
College of Resources, Sichuan Agricultural University, Chengdu, China
Search for more papers by this authorZhiyuan Xin
College of Resources and Environment Engineering, Mianyang Normal University, Mianyang, China
Search for more papers by this authorQi Tao
College of Resources, Sichuan Agricultural University, Chengdu, China
Search for more papers by this authorQiquan Li
College of Resources, Sichuan Agricultural University, Chengdu, China
Key Laboratory of Investigation and Monitoring, Protection and Utilization for Cultivated Land Resources, Ministry of Natural Resources, Chengdu, China
Search for more papers by this authorBing Li
College of Resources, Sichuan Agricultural University, Chengdu, China
Key Laboratory of Investigation and Monitoring, Protection and Utilization for Cultivated Land Resources, Ministry of Natural Resources, Chengdu, China
Search for more papers by this authorRong Huang
College of Resources, Sichuan Agricultural University, Chengdu, China
Key Laboratory of Investigation and Monitoring, Protection and Utilization for Cultivated Land Resources, Ministry of Natural Resources, Chengdu, China
Search for more papers by this authorQiang Xu
College of Resources, Sichuan Agricultural University, Chengdu, China
Search for more papers by this authorHuanxiu Li
Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, China
Search for more papers by this authorCorresponding Author
Changquan Wang
College of Resources, Sichuan Agricultural University, Chengdu, China
Key Laboratory of Investigation and Monitoring, Protection and Utilization for Cultivated Land Resources, Ministry of Natural Resources, Chengdu, China
Correspondence
Changquan Wang, Number 211, Huimin Road, Wenjiang District, Chengdu City, Sichuan Province, 611130, China.
Email: [email protected]
Search for more papers by this authorJie Shen is co-first author.
Abstract
Rapid urbanization drives changes in suburban cropland and afforestation, but the impact on soil microbes and carbon (C) dynamics remains unclear. Using genetic sequencing (16S rRNA sequence and functional prediction), we assessed the effects of cropland intensification and afforestation on the soil microbial communities and C sequestration potential in a suburban agricultural area. Traditional rotation (rice–wheat) homogenized microbial diversity along soil depth (0–100 cm). Cropland intensification (rice–wheat to rice–vegetables) and afforestation (rice–wheat to forest) decreased fungal diversity below 20 cm, while bacterial diversity and dominant taxa were less affected, due to the advantages of bacterial diversity and dominant taxa distribution. Cropland intensification increased profile C accumulation, mainly due to excessive fertilization (obviously decreased aerobic_chemoheterotrophy below 20 cm in bacterial function), but not C stability (significantly increased permanganate oxidizable C (POXC), and microbial-dependent C sources tend to from crop litter to soil sources). Afforestation offset C accumulation through no fertilization and inundation, but increased saprophytic function for C sequestration. In addition, pH and C had opposing effects on dominant microbial taxa, mitigating extreme differentiation. Subsoil was more sensitive to changes than topsoil. Altogether, soil C stability decoupled from C accumulation in the suburban agricultural area. Cropland intensification increased C accumulation yet decreased bacterial C sequestration potential and C stability; cropland afforestation facilitated fungi-derived C accumulation and its C sequestration. Deep soil C sequestration was more vulnerable to suburban agriculture practices, emphasizing the need to consider subsoil C-cycling in agricultural practices amid urbanization.
Open Research
DATA AVAILABILITY STATEMENT
Data available on request from the authors.
Supporting Information
| Filename | Description |
|---|---|
| ldr4984-sup-0001-Figures.docxWord 2007 document , 1.1 MB | FIGURE S1. Land use change effect on fungal community in 20–40 cm layer. FIGURE S2. Soil microbial quantity in the soil profile across different land use. Values followed by a different letters are significant difference among land use types in the same soil depth (p < 0.05). FIGURE S3. Microbial community composition of bacterial (a) and fungal (b) on class level (>0.1%, top 30). |
| ldr4984-sup-0002-Supinfo.docxWord 2007 document , 16 KB | Appendix S1. Supporting information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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