Original Report

Prognostic Impact of Tumor-Infiltrating Lymphocytes in Laryngeal Squamous Cell Carcinoma Patients

Duo Zhang MD

Department of Otolaryngology–HNS, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of Medicine, Shanghai, People's Republic of China

Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, People's Republic of China

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Di Tang MD,

Di Tang MD

Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, People's Republic of China

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Yu Heng MD,

Yu Heng MD

Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, People's Republic of China

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Xiao-Ke Zhu MD,

Xiao-Ke Zhu MD

Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, People's Republic of China

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Liang Zhou MD,

Liang Zhou MD

Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, People's Republic of China

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Lei Tao MD,

Corresponding Author

Lei Tao MD

[email protected]

orcid.org/0000-0003-3384-9396

Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, People's Republic of China

Send correspondence to Lei Tao, Department of Otolaryngology, Eye Ear Nose and Throat Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, People's Republic of China. E-mail: [email protected] or Li-ming Lu, Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, 280 South Chongqing Road, Shanghai 200025, People's Republic of China. E-mail: [email protected]

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Li-ming Lu PhD,

Corresponding Author

Li-ming Lu PhD

[email protected]

Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China

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Duo Zhang MD,

Duo Zhang MD

Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, People's Republic of China

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Di Tang MD,

Di Tang MD

Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, People's Republic of China

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Yu Heng MD,

Yu Heng MD

Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, People's Republic of China

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Xiao-Ke Zhu MD,

Xiao-Ke Zhu MD

Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, People's Republic of China

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Liang Zhou MD,

Liang Zhou MD

Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, People's Republic of China

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Lei Tao MD,

Corresponding Author

Lei Tao MD

[email protected]

orcid.org/0000-0003-3384-9396

Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, People's Republic of China

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Li-ming Lu PhD,

Corresponding Author

Li-ming Lu PhD

[email protected]

Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China

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First published: 27 October 2020

https://doi.org/10.1002/lary.29196

Citations: 23

Editor's Note: This Manuscript was accepted for publication on October 8, 2020.

Duo Zhang and Di Tang are co-first authors.l.z., l.t., l.-.l., designed study, revised article; d.z., d.t., analyzed data, drafting; x.-k. z., y.h., collected data, revised article. This study was supported by the Science and Technology Innovation Project of Shanghai Shen-kang Hospital Clinical Development Center under Grant (SHDC12015114); the Science and Technology Commission of Shanghai Municipality under Grant (16411950100); the National Natural Science Foundation of China under Grant (81772878, 30801283, 30972691); the Shanghai Science and Technology Development Funds under Grant (09QA1401000, 10QA1405900, 14411961900); the Training Program of the Excellent Young Talents of Shanghai Municipal Health System under Grant (XYQ2011055, XYQ2011015); and the Shanghai Municipal Science and Technology Foundation under Grant (11JC1410802). The authors have no other funding, financial relationships, or conflicts of interest to disclose.

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Abstract

Objectives/Hypothesis

Tumor-infiltrating lymphocytes (TILs) has been shown to be associated with the prognosis of many tumors, yet few studies have investigated their roles in laryngeal squamous cell carcinoma (LSCC). We aim to investigate the prognostic values of tumor-infiltrating CD3⁺/CD4⁺/CD8⁺/Foxp3⁺ T-cells and neutrophils in LSCC patients that received total or partial laryngectomy.

Study Design

Retrospective case series of LSCC patients who underwent total or partial laryngectomy from 2013 to 2014 at Eye, Ear, Nose, and Throat Hospital of Fudan University.

Methods

In our study, 41 tumor tissues from patients with LSCC were retrospectively assessed using immunohistochemistry for CD3⁺/CD4⁺/CD8⁺/Foxp3⁺ T-cells and CD66b⁺ neutrophils. Overall survival (OS) and disease-free survival (DFS) were recorded using Kaplan–Meier methods.

Results

Generally, patients with high density of TILs (CD3, CD4, CD8) showed improved OS or DFS. Specifically, high density of CD3⁺ TILs were associated with better OS, yet poorer OS and DFS for CD66b⁺ neutrophils. Patients with an Immunoscore of 0–1 experienced the worst OS and DFS, compared with Immunoscore 2–4 (P = .0111 for OS, P = .0391 for DFS). In Cox proportional hazards analysis adjusted for N stage and T stage, only stroma CD66b⁺ neutrophils densities were able to predict OS, with odds ratios of 4.819 (95% confidence interval [CI] 1.149–20.206; P = .032*), and DFS 2.888 (95% CI 1.043–7.997; P = .041*).

Conclusions

The density of TILs and CD66b⁺ neutrophils may help predict the prognosis of patients with LSCC after surgery.

Level of Evidence

3 Laryngoscope, 131:E1249–E1255, 2021

Supporting Information

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Citing Literature

Volume131, Issue4

April 2021

Pages E1249-E1255

Filename	Description
lary29196-sup-0001-FigureS1.tifTIFF image, 3.7 MB	Figure S1. Kaplan–Meier survival analysis of Foxp3+ with OS and DFS of patients with LSCC. OS according to Foxp3+ in intratumor area (A), in tumor stroma (B). DFS according to Foxp3+ in intratumor area (C), in tumor stroma (D). No significant association was found between the density of Foxp3 and the prognosis of LSCC patients.
lary29196-sup-0002-FigureS2.tifTIFF image, 13 MB	Figure S2. Representative examples of Foxp3+ in LSCC
lary29196-sup-0003-FigureS3.tifTIFF image, 18.6 MB	Figure S3. Kaplan–Meier survival analysis of CD3, CD4, CD8, and CD66b with Disease-free Survival (DFS) of patients with LSCC.
lary29196-sup-0004-TableS1.docxWord 2007 document , 16.2 KB	Table S1. The association between the density of TILs and clinical factors.
lary29196-sup-0005-TableS2.docxWord 2007 document , 13.8 KB	Table S2. The relationship between CD66b and other TILs.

Prognostic Impact of Tumor-Infiltrating Lymphocytes in Laryngeal Squamous Cell Carcinoma Patients

Abstract

Objectives/Hypothesis

Study Design

Methods

Results

Conclusions

Level of Evidence

Supporting Information

BIBLIOGRAPHY

Citing Literature

References

Information

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Prognostic Impact of Tumor-Infiltrating Lymphocytes in Laryngeal Squamous Cell Carcinoma Patients

Abstract

Objectives/Hypothesis

Study Design

Methods

Results

Conclusions

Level of Evidence

Supporting Information

BIBLIOGRAPHY

Citing Literature

References

Related

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