Laryngology

Cryotherapy has antifibrotic and regenerative effects on human vocal fold fibroblasts

Ting Gong MD

Department of Otolaryngology–Head and Neck Surgery, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China

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Chi Zhang MD,

Chi Zhang MD

orcid.org/0000-0002-6729-1426

Department of Otolaryngology–Head and Neck Surgery, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China

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Jing Kang MD,

Jing Kang MD

Department of Otolaryngology–Head and Neck Surgery, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China

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JJ Lamb,

JJ Lamb

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Jack J. Jiang MD, PhD,

Corresponding Author

Jack J. Jiang MD, PhD

[email protected]

orcid.org/0000-0002-3070-4952

Department of Otolaryngology–Head and Neck Surgery, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China

Department of Surgery, Division of Otolaryngology–Head and Neck Surgery, University of Wisconsin-Madison School of Medicine and Public Health, Madison, Wisconsin, U.S.A.

Send correspondence to Jack J. Jiang, MD, PhD, Department of Otolaryngology–Head and Neck Surgery, Eye, Ear, Nose, and Throat Hospital of Fudan University, 83 Fenyang Road, Shanghai, China 200031. E-mail: [email protected].Search for more papers by this author

Ting Gong MD,

Ting Gong MD

Department of Otolaryngology–Head and Neck Surgery, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China

Search for more papers by this author

Chi Zhang MD,

Chi Zhang MD

orcid.org/0000-0002-6729-1426

Department of Otolaryngology–Head and Neck Surgery, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China

Search for more papers by this author

Jing Kang MD,

Jing Kang MD

Department of Otolaryngology–Head and Neck Surgery, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China

Search for more papers by this author

JJ Lamb,

JJ Lamb

Search for more papers by this author

Jack J. Jiang MD, PhD,

Corresponding Author

Jack J. Jiang MD, PhD

[email protected]

orcid.org/0000-0002-3070-4952

Department of Otolaryngology–Head and Neck Surgery, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China

Department of Surgery, Division of Otolaryngology–Head and Neck Surgery, University of Wisconsin-Madison School of Medicine and Public Health, Madison, Wisconsin, U.S.A.

First published: 12 October 2018

https://doi.org/10.1002/lary.27499

Citations: 4

This work was supported by a grant from the National Natural Science Foundation of China, project 81329001.

The authors have no other funding, financial relationships, or conflicts of interest to disclose.

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Abstract

Objectives/Hypothesis

Vocal fold scarring remains a major treatment challenge, and scar prevention without residual lesions remains a dilemma. Cryotherapy has shown cosmetic outcomes on skin lesions with minimal scarring. The aim of this study was to clarify the beneficial effects of cryotherapy for the prevention and the treatment of vocal fold scarring.

Study Design

In vitro.

Methods

Primary cultures of human vocal fold fibroblasts (VFFs) were used in this study. Myofibroblast differentiation was stimulated by transforming growth factor β1 (TGF-β1). We mimicked the cryotherapy effect on vocal fold healing in vivo by freezing VFFs ± TGF-β1 in vitro. The influence of freezing on cell viability, proliferation, migration, and contractile properties were analyzed. The expression of collagen I, collagen III, fibronectin, TGF-β1, matrix metallopeptidase 1 (MMP1), hyaluronan synthase 1 (HAS1) were investigated by real-time polymerase chain reaction (RT-PCR), and the expression of alpha smooth muscle actin (α-SMA) and decorin were investigated by RT-PCR and Western blot.

Results

Freezing was found to modify extracellular matrix (ECM) synthesis and differentiation of VFFs. Expression of collagen I, collagen III, fibronectin, α-SMA, and TGF-β1 was downregulated, and MMP1 was upregulated in VFFs + TGF-β1 (myofibroblast) by freezing. HAS1 and decorin were upregulated in both VFFs ± TGF-β1 by freezing. Freezing VFFs + TGF-β1 (myofibroblast) with fast thawing had a lower expression of α-SMA when compared with slow thawing. Freezing reduced the migration and collagen contraction of VFFs + TGF-β1 (myofibroblast).

Conclusion

Cryotherapy induces antifibrotic and regenerative ECM alterations in VFFs. These data provide insight into the prevention and the treatment of vocal fold scarring with cryotherapy in phonomicrosurgery.

Level of Evidence

NA Laryngoscope, 129:E143–E150, 2019

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Citing Literature

Volume129, Issue4

April 2019

Pages E143-E150

Cryotherapy has antifibrotic and regenerative effects on human vocal fold fibroblasts

Abstract

Objectives/Hypothesis

Study Design

Methods

Results

Conclusion

Level of Evidence

BIBLIOGRAPHY

Citing Literature

References

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Cryotherapy has antifibrotic and regenerative effects on human vocal fold fibroblasts

Abstract

Objectives/Hypothesis

Study Design

Methods

Results

Conclusion

Level of Evidence

BIBLIOGRAPHY

Citing Literature

References

Related

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