Allergy/Rhinology

Cytokine correlation between sinus tissue and nasal secretions among chronic rhinosinusitis and controls

Samuel L. Oyer MD

Division of Rhinology&Sinus Surgery, Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, South Carolina

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Jennifer K. Mulligan PhD,

Jennifer K. Mulligan PhD

Division of Rhinology&Sinus Surgery, Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, South Carolina

Research Service, Ralph H. Johnson VA Medical Center, Charleston, South Carolina

Rhinology Division, Department of Otolaryngology–Head and Neck Surgery, Stanford Medical Center, Stanford, California, U.S.A.

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Alkis J. Psaltis MD, PhD,

Alkis J. Psaltis MD, PhD

Rhinology Division, Department of Otolaryngology–Head and Neck Surgery, Stanford Medical Center, Stanford, California, U.S.A.

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Oswaldo A. Henriquez MD,

Oswaldo A. Henriquez MD

Division of Rhinology&Sinus Surgery, Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, South Carolina

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Rodney J. Schlosser MD,

Corresponding Author

Rodney J. Schlosser MD

Division of Rhinology&Sinus Surgery, Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, South Carolina

Research Service, Ralph H. Johnson VA Medical Center, Charleston, South Carolina

Send correspondence to Rodney J. Schlosser, MD, MSC 550, 135 Rutledge Ave. Charleston, SC 29425. E-mail: [email protected]Search for more papers by this author

Samuel L. Oyer MD,

Samuel L. Oyer MD

Division of Rhinology&Sinus Surgery, Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, South Carolina

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Jennifer K. Mulligan PhD,

Jennifer K. Mulligan PhD

Division of Rhinology&Sinus Surgery, Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, South Carolina

Research Service, Ralph H. Johnson VA Medical Center, Charleston, South Carolina

Rhinology Division, Department of Otolaryngology–Head and Neck Surgery, Stanford Medical Center, Stanford, California, U.S.A.

Search for more papers by this author

Alkis J. Psaltis MD, PhD,

Alkis J. Psaltis MD, PhD

Rhinology Division, Department of Otolaryngology–Head and Neck Surgery, Stanford Medical Center, Stanford, California, U.S.A.

Search for more papers by this author

Oswaldo A. Henriquez MD,

Oswaldo A. Henriquez MD

Division of Rhinology&Sinus Surgery, Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, South Carolina

Search for more papers by this author

Rodney J. Schlosser MD,

Corresponding Author

Rodney J. Schlosser MD

Division of Rhinology&Sinus Surgery, Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, South Carolina

Research Service, Ralph H. Johnson VA Medical Center, Charleston, South Carolina

Send correspondence to Rodney J. Schlosser, MD, MSC 550, 135 Rutledge Ave. Charleston, SC 29425. E-mail: [email protected]Search for more papers by this author

First published: 12 July 2013

https://doi.org/10.1002/lary.24305

Citations: 68

Funded in part by grants to R.J.S. (grant 113039) and J.K.M. (grant 092401) from the Flight Attendants Medical Research Institute, and to R.J.S. from the Department of Veterans Affairs. R.J.S. is a consultant for BrainLAB, Olympus, and Sunovion and received previous grant support from Medtronic, Arthrocare, NeilMed, and the speaker's bureau for NeilMed. The authors have no other funding, financial relationships, or conflicts of interest to disclose.

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Abstract

Objectives/Hypothesis

Compare cytokine levels in sinus tissue to sinus secretions from controls and chronic rhinosinusitis patients.

Study Design

In vitro.

Methods

Polyurethane foam sponges were placed into middle meati of patients with chronic rhinosinusitis without nasal polyps (CRSsNP), with polyps (CRSwNP), and controls. Sinus biopsies were then taken from the same location. Protein levels of tumor necrosis factor-alpha (TNF-α), interferon-γ (IFN-γ), and interleukins (IL) 2, 4, 6, 8, 10, and 17A were measured via cytometric bead assay for each sample. Protein values from sinus tissue and secretions were compared with Pearson's correlation between samples as well as one-way ANOVA with posthoc t test between groups.

Results

Samples from 43 patients in total were examined. Mucus was measured from 10 controls, 11 CRSsNP and 10 CRSwNP, and sinus tissue was measured from 10 controls, 15 CRSsNP and 10 CRSwNP. IL-8 and IFN-γ levels were outside of the detectable range of the assay. Levels of secreted IL-2, 4, 6, 10, and 17A correlated with tissue levels (P < 0.05 for all, r > 0.49) while TNF-α did not (P = 0.71). CRSsNP had elevated mucus levels of IL-2, 4, 6, 10, and 17A compared to controls. CRSwNP had elevated mucus levels of IL-4, 6, 10, 17A, and TNF-α compared to controls.

Conclusions

Cytokine levels in sinus secretions correlate with levels in sinus tissue and are elevated in CRS versus control based on Th1/Th2 skewing.

Level of Evidence

N/A (in vitro). Laryngoscope, 123:E72–E78, 2013

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Citing Literature

Volume123, Issue12

December 2013

Pages E72-E78

Cytokine correlation between sinus tissue and nasal secretions among chronic rhinosinusitis and controls

Abstract

Objectives/Hypothesis

Study Design

Methods

Results

Conclusions

Level of Evidence

BIBLIOGRAPHY

Citing Literature

References

Information

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Cytokine correlation between sinus tissue and nasal secretions among chronic rhinosinusitis and controls

Abstract

Objectives/Hypothesis

Study Design

Methods

Results

Conclusions

Level of Evidence

BIBLIOGRAPHY

Citing Literature

References

Related

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