Quantitative determination of conjugated estrogens in formulations by capillary GLC

G. K. Pillai,

G. K. Pillai

Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, V6T 1W5 Canada

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K. M. McErlane,

Corresponding Author

K. M. McErlane

Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, V6T 1W5 Canada

Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, V6T 1W5 CanadaSearch for more papers by this author

G. K. Pillai,

G. K. Pillai

Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, V6T 1W5 Canada

Search for more papers by this author

K. M. McErlane,

Corresponding Author

K. M. McErlane

Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, V6T 1W5 Canada

Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, V6T 1W5 CanadaSearch for more papers by this author

First published: September 1981

https://doi.org/10.1002/jps.2600700927

Citations: 4

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Abstract

A rapid capillary GLC method for the analysis of conjugated estrogen tablets and injectable formulations is described. The method involves the hydrolytic cleavage of the sodium sulfate ester conjugates by sulfatase enzyme. The free phenolic steroids are reacted sequentially with hydroxylamine hydrochloride and N, O-bis(trimethylsilyl)trifluoroacetamide. The resulting dual derivatives are analyzed on a 15-m glass capillary column wall coated with a cyanopropylmethyl silicone phase.

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Volume70, Issue9

September 1981

Pages 1072-1075

Quantitative determination of conjugated estrogens in formulations by capillary GLC

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Quantitative determination of conjugated estrogens in formulations by capillary GLC

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