A comparative study of the antigen-specific immune response induced by co-delivery of CpG ODN and antigen using fusion molecules or biodegradable microparticles†
Xue-Qing Zhang
Division of Pharmaceutics, College of Pharmacy, University of Iowa, S228 Pharmacy Building, 115 S. Grand Avenue, Iowa City, Iowa 52242
Search for more papers by this authorChristopher E. Dahle
Holden Comprehensive Cancer Center and Departmental of Internal Medicine, University of Iowa, Iowa City, Iowa, 52242
Search for more papers by this authorGeorge J. Weiner
Holden Comprehensive Cancer Center and Departmental of Internal Medicine, University of Iowa, Iowa City, Iowa, 52242
Search for more papers by this authorCorresponding Author
Aliasger K. Salem
Division of Pharmaceutics, College of Pharmacy, University of Iowa, S228 Pharmacy Building, 115 S. Grand Avenue, Iowa City, Iowa 52242
Holden Comprehensive Cancer Center and Departmental of Internal Medicine, University of Iowa, Iowa City, Iowa, 52242
Division of Pharmaceutics, College of Pharmacy, University of Iowa, S228 Pharmacy Building, 115 S. Grand Avenue, Iowa City, Iowa 52242. Telephone: 3193358810; Fax: 3193359349Search for more papers by this authorXue-Qing Zhang
Division of Pharmaceutics, College of Pharmacy, University of Iowa, S228 Pharmacy Building, 115 S. Grand Avenue, Iowa City, Iowa 52242
Search for more papers by this authorChristopher E. Dahle
Holden Comprehensive Cancer Center and Departmental of Internal Medicine, University of Iowa, Iowa City, Iowa, 52242
Search for more papers by this authorGeorge J. Weiner
Holden Comprehensive Cancer Center and Departmental of Internal Medicine, University of Iowa, Iowa City, Iowa, 52242
Search for more papers by this authorCorresponding Author
Aliasger K. Salem
Division of Pharmaceutics, College of Pharmacy, University of Iowa, S228 Pharmacy Building, 115 S. Grand Avenue, Iowa City, Iowa 52242
Holden Comprehensive Cancer Center and Departmental of Internal Medicine, University of Iowa, Iowa City, Iowa, 52242
Division of Pharmaceutics, College of Pharmacy, University of Iowa, S228 Pharmacy Building, 115 S. Grand Avenue, Iowa City, Iowa 52242. Telephone: 3193358810; Fax: 3193359349Search for more papers by this authorXue-Qing Zhang and Christopher E. Dahle contributed equally to this work.
Abstract
CpG ODN are toll-like receptor 9 (TLR9) agonists that can enhance antigen presentation by antigen presenting cells (APCs) such as dendritic cells (DCs). The most potent antigen-specific responses are seen when CpG ODN and the antigen are co-localized in the same APC. CpG ODN–antigen fusion molecules and biodegradable microparticles entrapping CpG ODN and antigen can ensure both components are delivered to the same APC. In this study, we compared the efficacy of the CpG–ODN fusion molecules against biodegradable microparticles entrapping antigen and CpG ODN. Microparticles were prepared using a double emulsion solvent evaporation methodology. CpG ODN–OVA fusion molecules were prepared by mixing maleimide-activated protein with thiolated CpG ODN. Both CpG ODN–OVA fusion molecules and microparticles co-entrapping CpG ODN and OVA generated stronger IgG2a and interferon-gamma (IFN-γ) responses than delivery of soluble CpG ODN and OVA. The microparticles generated stronger IgG2a and IFN-γ immune responses than did CpG ODN-antigen fusion molecules. © 2007 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 96: 3283–3292, 2007
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