Fabrication and characterization of electrospun laminin-functionalized silk fibroin/poly(ethylene oxide) nanofibrous scaffolds for peripheral nerve regeneration
Mina Rajabi
Department of Polymer, School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran
Search for more papers by this authorCorresponding Author
Masoumeh Firouzi
Tissue Repair Laboratory, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran
Correspondence to: M. Firouzi; e-mail: [email protected] or P. Zahedi; e-mail: [email protected]Search for more papers by this authorZahra Hassannejad
Pediatric Urology and Regenerative Medicine Research Center, Children's Hospital Medical Center, Tehran University of Medical Sciences, Tehran, Iran
Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran
Search for more papers by this authorIsmaeil Haririan
Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy and Department of Pharmaceutics, Tehran University of Medical Sciences, Tehran, P. O. Box: 14155-6451 Tehran, Iran
Search for more papers by this authorCorresponding Author
Payam Zahedi
Department of Polymer, School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran
Correspondence to: M. Firouzi; e-mail: [email protected] or P. Zahedi; e-mail: [email protected]Search for more papers by this authorMina Rajabi
Department of Polymer, School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran
Search for more papers by this authorCorresponding Author
Masoumeh Firouzi
Tissue Repair Laboratory, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran
Correspondence to: M. Firouzi; e-mail: [email protected] or P. Zahedi; e-mail: [email protected]Search for more papers by this authorZahra Hassannejad
Pediatric Urology and Regenerative Medicine Research Center, Children's Hospital Medical Center, Tehran University of Medical Sciences, Tehran, Iran
Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran
Search for more papers by this authorIsmaeil Haririan
Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy and Department of Pharmaceutics, Tehran University of Medical Sciences, Tehran, P. O. Box: 14155-6451 Tehran, Iran
Search for more papers by this authorCorresponding Author
Payam Zahedi
Department of Polymer, School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran
Correspondence to: M. Firouzi; e-mail: [email protected] or P. Zahedi; e-mail: [email protected]Search for more papers by this authorAbstract
The peripheral nerve regeneration is still one of the major clinical problems, which has received a great deal of attention. In this study, the electrospun silk fibroin (SF)/poly(ethylene oxide) (PEO) nanofibrous scaffolds were fabricated and functionalized their surfaces with laminin (LN) without chemical linkers for potential use in the peripheral nerve tissue engineering. The morphology, surface chemistry, thermal behavior and wettability of the scaffolds were examined to evaluate their performance by means of scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC) and water contact angle (WCA) measurements, respectively. The proliferation and viability of Schwann cells onto the surfaces of SF/PEO nanofibrous scaffolds were investigated using SEM and thiazolyl blue (MTT) assay. The results showed an improvement of SF conformation and surface hydrophilicity of SF/PEO nanofibers after methanol and O2 plasma treatments. The immunostaining observation indicated a continuous coating of LN on the scaffolds. Improving the surface hydrophilicity and LN functionalization significantly increased the cell proliferation and this was more prominent after 5 days of culture time. In conclusion, the obtained results revealed that the electrospun LN-functionalized SF/PEO nanofibrous scaffold could be a promising candidate for peripheral nerve tissue regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1595–1604, 2018.
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