Original Research Report
Reduced cell attachment to poly(2-hydroxyethyl methacrylate)-coated ventricular catheters in vitro
Brian W. Hanak,
Chia-Yun Hsieh,
William Donaldson,
Samuel R. Browd,
Kenneth K. S. Lau,
William Shain,
Corresponding Author
Brian W. Hanak
Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington
Department of Neurological Surgery, University of Washington, Seattle, Washington
Brian W. Hanak and Chia-Yun Hsieh are co-first authors.
Correspondence to: Brian W. Hanak, e-mail: [email protected]Search for more papers by this authorChia-Yun Hsieh
Department of Chemical and Biological Engineering, Drexel University, Philadelphia, Pennsylvania
Brian W. Hanak and Chia-Yun Hsieh are co-first authors.
Search for more papers by this authorWilliam Donaldson
Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington
Search for more papers by this authorSamuel R. Browd
Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington
Department of Neurological Surgery, University of Washington, Seattle, Washington
Search for more papers by this authorKenneth K. S. Lau
Department of Chemical and Biological Engineering, Drexel University, Philadelphia, Pennsylvania
Search for more papers by this authorWilliam Shain
Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington
Search for more papers by this authorBrian W. Hanak,
Chia-Yun Hsieh,
William Donaldson,
Samuel R. Browd,
Kenneth K. S. Lau,
William Shain,
Corresponding Author
Brian W. Hanak
Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington
Department of Neurological Surgery, University of Washington, Seattle, Washington
Brian W. Hanak and Chia-Yun Hsieh are co-first authors.
Correspondence to: Brian W. Hanak, e-mail: [email protected]Search for more papers by this authorChia-Yun Hsieh
Department of Chemical and Biological Engineering, Drexel University, Philadelphia, Pennsylvania
Brian W. Hanak and Chia-Yun Hsieh are co-first authors.
Search for more papers by this authorWilliam Donaldson
Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington
Search for more papers by this authorSamuel R. Browd
Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington
Department of Neurological Surgery, University of Washington, Seattle, Washington
Search for more papers by this authorKenneth K. S. Lau
Department of Chemical and Biological Engineering, Drexel University, Philadelphia, Pennsylvania
Search for more papers by this authorWilliam Shain
Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington
Search for more papers by this authorAbstract
The majority of patients with hydrocephalus are dependent on ventriculoperitoneal shunts for diversion of excess cerebrospinal fluid. Unfortunately, these shunts are failure-prone and over half of all life-threatening pediatric failures are caused by obstruction of the ventricular catheter by the brain's resident immune cells, reactive microglia and astrocytes. Poly(2-hydroxyethyl methacrylate) (PHEMA) hydrogels are widely used for biomedical implants. The extreme hydrophilicity of PHEMA confers resistance to protein fouling, making it a strong candidate coating for ventricular catheters. With the advent of initiated chemical vapor deposition (iCVD), a solvent-free coating technology that creates a polymer in thin film form on a substrate surface by introducing gaseous reactant species into a vacuum reactor, it is now possible to apply uniform polymer coatings on complex three-dimensional substrate surfaces. iCVD was utilized to coat commercially available ventricular catheters with PHEMA. The chemical structure was confirmed on catheter surfaces using Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. PHEMA coating morphology was characterized by scanning electron microscopy. Testing PHEMA-coated catheters against uncoated clinical-grade catheters in an in vitro hydrocephalus catheter bioreactor containing co-cultured astrocytes and microglia revealed significant reductions in cell attachment to PHEMA-coated catheters at both 17-day and 6-week time points. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1268–1279, 2018.
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