Microenvironment influences vascular differentiation of murine cardiovascular progenitor cells
Jessica M. Gluck
Department of Surgery, Cardiovascular Tissue Engineering Laboratory, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California
Department of Medicine, Cardiovascular Research Laboratories, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California
Search for more papers by this authorConnor Delman
Department of Surgery, Cardiovascular Tissue Engineering Laboratory, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California
Search for more papers by this authorJennifer Chyu
Department of Medicine, Cardiovascular Research Laboratories, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California
Search for more papers by this authorW. Robb MacLellan
Department of Medicine, Cardiovascular Research Laboratories, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California
Division of Cardiology, School of Medicine, University of Washington, Seattle, Washington
Search for more papers by this authorRichard J. Shemin
Department of Surgery, Cardiovascular Tissue Engineering Laboratory, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California
Search for more papers by this authorCorresponding Author
Sepideh Heydarkhan-Hagvall
Department of Surgery, Cardiovascular Tissue Engineering Laboratory, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California
AstraZeneca R&D Mölndal, Cardiovascular and Metabolic Diseases iMed, Mölndal, Sweden
Correspondence to: S. Heydarkhan-Hagvall ([email protected])Search for more papers by this authorJessica M. Gluck
Department of Surgery, Cardiovascular Tissue Engineering Laboratory, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California
Department of Medicine, Cardiovascular Research Laboratories, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California
Search for more papers by this authorConnor Delman
Department of Surgery, Cardiovascular Tissue Engineering Laboratory, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California
Search for more papers by this authorJennifer Chyu
Department of Medicine, Cardiovascular Research Laboratories, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California
Search for more papers by this authorW. Robb MacLellan
Department of Medicine, Cardiovascular Research Laboratories, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California
Division of Cardiology, School of Medicine, University of Washington, Seattle, Washington
Search for more papers by this authorRichard J. Shemin
Department of Surgery, Cardiovascular Tissue Engineering Laboratory, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California
Search for more papers by this authorCorresponding Author
Sepideh Heydarkhan-Hagvall
Department of Surgery, Cardiovascular Tissue Engineering Laboratory, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California
AstraZeneca R&D Mölndal, Cardiovascular and Metabolic Diseases iMed, Mölndal, Sweden
Correspondence to: S. Heydarkhan-Hagvall ([email protected])Search for more papers by this authorAbstract
We examined the effects of the microenvironment on vascular differentiation of murine cardiovascular progenitor cells (CPCs). We isolated CPCs and seeded them in culture exposed to the various extracellular matrix (ECM) proteins in both two-dimensional (2D) and 3D culture systems. To better understand the contribution of the microenvironment to vascular differentiation, we analyzed endothelial and smooth muscle cell differentiation at both day 7 and day 14. We found that laminin and vitronectin enhanced vascular endothelial cell differentiation while fibronectin enhanced vascular smooth muscle cell differentiation. We also observed that the effects of the 3D electrospun scaffolds were delayed and not noticeable until the later time point (day 14), which may be due to the amount of time necessary for the cells to migrate to the interior of the scaffold. The study characterized the contributions of both ECM proteins and the addition of a 3D culture system to continued vascular differentiation. Additionally, we demonstrated the capability bioengineer a CPC-derived vascular graft. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 1730–1739, 2014.
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