Hydrothermal fabrication of hydroxyapatite/chitosan/carbon porous scaffolds for bone tissue engineering
Teng Long
Shanghai Key Laboratory of Orthopaedic Implant Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011 China
Both authors contributed equally to this work.
Search for more papers by this authorYu-Tai Liu
The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai, 200234 China
The Education Ministry Engineering Research Center of Materials Composition and Advanced Dispersion Technology, Shanghai University, Shanghai, 200072 China
Both authors contributed equally to this work.
Search for more papers by this authorSha Tang
The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai, 200234 China
Search for more papers by this authorJin-Liang Sun
The Education Ministry Engineering Research Center of Materials Composition and Advanced Dispersion Technology, Shanghai University, Shanghai, 200072 China
Search for more papers by this authorYa-Ping Guo
The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai, 200234 China
Search for more papers by this authorCorresponding Author
Zhen-An Zhu
Shanghai Key Laboratory of Orthopaedic Implant Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011 China
Correspondence to: Z.-A. Zhu (e-mail: [email protected]) or Y.-P. Guo (e-mail: [email protected])Search for more papers by this authorTeng Long
Shanghai Key Laboratory of Orthopaedic Implant Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011 China
Both authors contributed equally to this work.
Search for more papers by this authorYu-Tai Liu
The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai, 200234 China
The Education Ministry Engineering Research Center of Materials Composition and Advanced Dispersion Technology, Shanghai University, Shanghai, 200072 China
Both authors contributed equally to this work.
Search for more papers by this authorSha Tang
The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai, 200234 China
Search for more papers by this authorJin-Liang Sun
The Education Ministry Engineering Research Center of Materials Composition and Advanced Dispersion Technology, Shanghai University, Shanghai, 200072 China
Search for more papers by this authorYa-Ping Guo
The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai, 200234 China
Search for more papers by this authorCorresponding Author
Zhen-An Zhu
Shanghai Key Laboratory of Orthopaedic Implant Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011 China
Correspondence to: Z.-A. Zhu (e-mail: [email protected]) or Y.-P. Guo (e-mail: [email protected])Search for more papers by this authorAbstract
Porous carbon fiber felts (PCFFs) have great applications in orthopedic surgery because of the strong mechanical strength, low density, high stability, and porous structure, but they are biologically inert. To improve their biological properties, we developed, for the first time, the hydroxyapatite (HA)/chitosan/carbon porous scaffolds (HCCPs). HA/chitosan nanohybrid coatings have been fabricated on PCFFs according to the following stages: (i) deposition of chitosan/calcium phosphate precursors on PCFFs; and (ii) hydrothermal transformation of the calcium phosphate precursors in chitosan matrix into HA nanocrystals. The scanning electron microscopy images indicate that PCFFs are uniformly covered with elongated HA nanoplates and chitosan, and the macropores in PCFFs still remain. Interestingly, the calcium-deficient HA crystals exist as plate-like shapes with thickness of 10–18 nm, width of 30–40 nm, and length of 80–120 nm, which are similar to the biological apatite. The HA in HCCPs is similar to the mineral of natural bone in chemical composition, crystallinity, and morphology. As compared with PCFFs, HCCPs exhibit higher in vitro bioactivity and biocompatibility because of the presence of the HA/chitosan nanohybrid coatings. HCCPs not only promote the formation of bone-like apatite in simulated body fluid, but also improve the adhesion, spreading, and proliferation of human bone marrow stromal cells. Hence, HCCPs have great potentials as scaffold materials for bone tissue engineering and implantation. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 1740–1748, 2014.
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