Biomechanical and antibacterial properties of Tobramycin loaded hydroxyapatite coated fixation pins
Jan Henrik Sörensen
Department of Pharmaceutics and Biopharmaceutics, Christian Albrecht University Kiel, 24118 Kiel, Germany
Search for more papers by this authorMirjam Lilja
Division for Nanotechnology and Functional Materials, The Ångström Laboratory, Uppsala University, 751 21 Uppsala Sweden
Sandvik Coromant Sverige AB, 12680 Stockholm, Sweden
Search for more papers by this authorTorben Christian Sörensen
Stryker Trauma GmbH, 24232 Schönkirchen, Germany
Search for more papers by this authorMaria Åstrand
Sandvik Coromant Sverige AB, 12680 Stockholm, Sweden
Search for more papers by this authorSabine Fuchs
Experimental Trauma Surgery, University Medical Center Schleswig-Holstein, Kiel, Germany
Search for more papers by this authorCorresponding Author
Maria Strømme
Division for Nanotechnology and Functional Materials, The Ångström Laboratory, Uppsala University, 751 21 Uppsala Sweden
Correspondence to: H. Steckel; e-mail: [email protected] or M. Strømme; e-mail: [email protected]Search for more papers by this authorCorresponding Author
Hartwig Steckel
Department of Pharmaceutics and Biopharmaceutics, Christian Albrecht University Kiel, 24118 Kiel, Germany
Correspondence to: H. Steckel; e-mail: [email protected] or M. Strømme; e-mail: [email protected]Search for more papers by this authorJan Henrik Sörensen
Department of Pharmaceutics and Biopharmaceutics, Christian Albrecht University Kiel, 24118 Kiel, Germany
Search for more papers by this authorMirjam Lilja
Division for Nanotechnology and Functional Materials, The Ångström Laboratory, Uppsala University, 751 21 Uppsala Sweden
Sandvik Coromant Sverige AB, 12680 Stockholm, Sweden
Search for more papers by this authorTorben Christian Sörensen
Stryker Trauma GmbH, 24232 Schönkirchen, Germany
Search for more papers by this authorMaria Åstrand
Sandvik Coromant Sverige AB, 12680 Stockholm, Sweden
Search for more papers by this authorSabine Fuchs
Experimental Trauma Surgery, University Medical Center Schleswig-Holstein, Kiel, Germany
Search for more papers by this authorCorresponding Author
Maria Strømme
Division for Nanotechnology and Functional Materials, The Ångström Laboratory, Uppsala University, 751 21 Uppsala Sweden
Correspondence to: H. Steckel; e-mail: [email protected] or M. Strømme; e-mail: [email protected]Search for more papers by this authorCorresponding Author
Hartwig Steckel
Department of Pharmaceutics and Biopharmaceutics, Christian Albrecht University Kiel, 24118 Kiel, Germany
Correspondence to: H. Steckel; e-mail: [email protected] or M. Strømme; e-mail: [email protected]Search for more papers by this authorAbstract
The present study investigates the use of nanoporous, biomimetic hydroxyapatite (HA) coatings deposited on TiO2 coated fixation pins as functional implant surfaces for the local release of Tobramycin in order to prevent bacterial colonization. The impact of HA-coating thickness, coating morphology and biomechanical forces during insertion into synthetic bone on the drug loading and release properties are analyzed. The coatings are shown to exhibit bactericidal effects against Staphylococcus aureus in agar medium for a duration of 6 days after loading by adsorption with Tobramycin for only 5 min at elevated temperature and pressure. Furthermore, high performance liquid chromatography analysis shows a drug release in phosphate buffered saline for 8 days with antibiotic concentration remaining above the minimal inhibitory concentration for S. aureus during the entire release period. Biomechanical insertion tests into synthetic bone and conventional scratch testing demonstrate adhesive strength at the HA/TiO2 interface. Biocompatibility is verified by cell viability tests. Outgrowth endothelial cells, as well as primary osteoblasts, are viable and firmly attached to both HA and TiO2 surfaces. The results presented are encouraging and support the concept of functional HA coatings as local drug delivery vehicles for biomedical applications to treat as well as to prevent post-surgical infections. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 1381–1392, 2014.
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