Cytotoxic response of three cell lines exposed in vitro to dental endodontic sealers
Corresponding Author
Martha Goël Brackett
Department of Oral Rehabilitation, Medical College of Georgia School of Dentistry, Augusta, Georgia
Department of Oral Rehabilitation, Medical College of Georgia School of Dentistry, Augusta, GeorgiaSearch for more papers by this authorRegina L. W. Messer
Department of Oral Biology, Medical College of Georgia School of Dentistry, Augusta, Georgia
Search for more papers by this authorPetra E. Lockwood
Department of Oral Biology, Medical College of Georgia School of Dentistry, Augusta, Georgia
Search for more papers by this authorThomas E. Bryan
Department of Oral Biology, Medical College of Georgia School of Dentistry, Augusta, Georgia
Search for more papers by this authorJill B. Lewis
Department of Oral Biology, Medical College of Georgia School of Dentistry, Augusta, Georgia
Search for more papers by this authorSerge Bouillaguet
Department of Cariology and Endodontology, University of Geneva, Switzerland
Search for more papers by this authorJohn C. Wataha
Department of Restorative Dentistry, University of Washington School of Dentistry, Seattle, Washington
Search for more papers by this authorCorresponding Author
Martha Goël Brackett
Department of Oral Rehabilitation, Medical College of Georgia School of Dentistry, Augusta, Georgia
Department of Oral Rehabilitation, Medical College of Georgia School of Dentistry, Augusta, GeorgiaSearch for more papers by this authorRegina L. W. Messer
Department of Oral Biology, Medical College of Georgia School of Dentistry, Augusta, Georgia
Search for more papers by this authorPetra E. Lockwood
Department of Oral Biology, Medical College of Georgia School of Dentistry, Augusta, Georgia
Search for more papers by this authorThomas E. Bryan
Department of Oral Biology, Medical College of Georgia School of Dentistry, Augusta, Georgia
Search for more papers by this authorJill B. Lewis
Department of Oral Biology, Medical College of Georgia School of Dentistry, Augusta, Georgia
Search for more papers by this authorSerge Bouillaguet
Department of Cariology and Endodontology, University of Geneva, Switzerland
Search for more papers by this authorJohn C. Wataha
Department of Restorative Dentistry, University of Washington School of Dentistry, Seattle, Washington
Search for more papers by this authorAbstract
The in vitro cytotoxicity of five endodontic sealers was measured >8-12 weeks using L929 mouse fibroblasts, osteoblastic cells (ROS) 17/2.8 rat osteoblasts, and MC3T3-E1 mouse osteoblasts. Discs (n = 6) of AH-plus Jet (AHP), two versions of Endo Rez (ER, ERx), Epiphany (EPH), and Pulp Canal Sealer (PCS) were prepared. The sealers and Teflon (Tf, negative control) were placed in direct contact with cells after immersion in phosphate-buffered saline for 1-12 wk. Cellular succinate dehydrogenase (SDH) activity was estimated using the MTT method (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole), and activities were normalized to Teflon® controls. The cellular responses to the materials were compared using analysis of variance with Tukey posthoc analyses (α = 0.05). Initially, all sealers suppressed normalized SDH activity of L929 fibroblasts by >90%. After 12 weeks of immersion in saline, AHP exhibited the SDH activity above Tf (120%), followed by ERx (78%), ER (58%), PCS (38%), and EPH (28%), all statistically distinct (p < 0.05). In general, the three cell lines responded similarly to the sealers. However, AHP caused unique responses: ROS cells were significantly (p < 0.05) less sensitive initially, and AHP was severely cytotoxic to MC3T3 cells (<35% of Tf) through 8 weeks. The data suggest that with “aging” in saline, current endodontic sealers decrease in in vitro cytotoxicity at different rates. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010.
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