Influence of nano-hydroxyapatite coating implants on gene expression of osteogenic markers and micro-CT parameters. An in vivo study in diabetic rats
Paula Gabriela Faciola Pessôa de Oliveira
Department of Oral and Maxillofacial Surgery and Periodontology, FORP/USP, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Search for more papers by this authorMariana Sales de Melo Soares
Department of Oral and Maxillofacial Surgery and Periodontology, FORP/USP, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Search for more papers by this authorAdriana Maria Mariano Silveira e Souza
Department of Oral and Maxillofacial Surgery and Periodontology, FORP/USP, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Search for more papers by this authorMário Taba Jr
Department of Oral and Maxillofacial Surgery and Periodontology, FORP/USP, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Search for more papers by this authorDaniela Bazan Palioto
Department of Oral and Maxillofacial Surgery and Periodontology, FORP/USP, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Search for more papers by this authorMichel Reis Messora
Department of Oral and Maxillofacial Surgery and Periodontology, FORP/USP, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Search for more papers by this authorBruna Ghiraldini
Paulista University, School of Dentistry, São Paulo, São Paulo, Brazil
Search for more papers by this authorFelipe Anderson de Sousa Nunes
Department of Oral and Maxillofacial Surgery and Periodontology, FORP/USP, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Search for more papers by this authorCorresponding Author
Sérgio Luís Scombatti de Souza
Department of Oral and Maxillofacial Surgery and Periodontology, FORP/USP, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Correspondence
Sérgio Luís Scombatti de Souza Departament of Oral & Maxillofacial Surgery and Periodontology School of Dentistry of Ribeirão Preto University of São Paulo Av do Café, s/n. 14040-904 Ribeirão Preto, SP, Brazil.
Email: [email protected]
Search for more papers by this authorPaula Gabriela Faciola Pessôa de Oliveira
Department of Oral and Maxillofacial Surgery and Periodontology, FORP/USP, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Search for more papers by this authorMariana Sales de Melo Soares
Department of Oral and Maxillofacial Surgery and Periodontology, FORP/USP, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Search for more papers by this authorAdriana Maria Mariano Silveira e Souza
Department of Oral and Maxillofacial Surgery and Periodontology, FORP/USP, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Search for more papers by this authorMário Taba Jr
Department of Oral and Maxillofacial Surgery and Periodontology, FORP/USP, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Search for more papers by this authorDaniela Bazan Palioto
Department of Oral and Maxillofacial Surgery and Periodontology, FORP/USP, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Search for more papers by this authorMichel Reis Messora
Department of Oral and Maxillofacial Surgery and Periodontology, FORP/USP, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Search for more papers by this authorBruna Ghiraldini
Paulista University, School of Dentistry, São Paulo, São Paulo, Brazil
Search for more papers by this authorFelipe Anderson de Sousa Nunes
Department of Oral and Maxillofacial Surgery and Periodontology, FORP/USP, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Search for more papers by this authorCorresponding Author
Sérgio Luís Scombatti de Souza
Department of Oral and Maxillofacial Surgery and Periodontology, FORP/USP, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Correspondence
Sérgio Luís Scombatti de Souza Departament of Oral & Maxillofacial Surgery and Periodontology School of Dentistry of Ribeirão Preto University of São Paulo Av do Café, s/n. 14040-904 Ribeirão Preto, SP, Brazil.
Email: [email protected]
Search for more papers by this authorThe authors have complete independence in the results and did not receive any kind of aid.
Funding information: National Council of Scientific and Technological Development (CNPq, Brazil), Grant/Award Number: Grant number: 446840/2014-9; State of Sao Paulo Research Foundation (FAPESP, Brazil), Grant/Award Numbers: 2015/09879-0, 2016/14584-2
Abstract
This study evaluated the response of a nano-hydroxyapatite coating implant through gene expression analysis (runt-related transcription factor 2 (Runx2), alkaline phosphatase (Alp), osteopontin (Opn), osteocalcin (Oc), receptor activator of nuclear factor-kappa B (Rank), receptor activator of nuclear factor-kappa B ligand (Rank-L), and osteoprotegerin (Opg)). Three-dimensional evaluation (percent bone volume (BV/TV); percent intersection surface (BIC); bone surface/volume ratio (BS/BV); and total porosity (To.Po)) were also analyzed. Mini implants were surgically placed in tibias of both healthy and diabetic rats. The animals were euthanized at 7 and 30 days. Evaluating all factors the relative expression of Rank showed that NANO surface presented the best results at 7 days (diabetic rats). Furthermore the levels of Runx2, Alp, Oc, and Opn suggest an increase in osteoblasts proliferation, especially in early stages of osseointegration. %BIC in healthy and diabetic (7 days) depicted statistically significant differences for NANO group. BV/TV, BS/BV and To.Po demonstrated higher values for NANO group in all evaluated time point and irrespective of systemic condition, but BS/BV 30 days (healthy rat) and 7 and 30 days (diabetic rat). Microtomographic and gene expression analyses have shown the benefits of nano-hydroxyapatite coated implants in promoting new bone formation in diabetic rats.
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