Effect of three-dimensional porosity gradients of biomimetic coatings on their bonding strength and cell behavior
Le Yu
Department of Materials Science and Engineering, University of Connecticut, Storrs, Connecticut, USA
Department of Chemical and Biomolecular Engineering, Ohio University, Athens, Ohio, USA
Search for more papers by this authorTomas M. Silva Santisteban
Department of Materials & Structure Analysis, Thermo Fisher Scientific, Houston, Texas, USA
Search for more papers by this authorQinqing Liu
Department of Computer Science and Engineering, University of Connecticut, Storrs, Connecticut, USA
Search for more papers by this authorChangmin Hu
Institute of Materials Science, University of Connecticut, Storrs, Connecticut, USA
Search for more papers by this authorJinbo Bi
Department of Computer Science and Engineering, University of Connecticut, Storrs, Connecticut, USA
Search for more papers by this authorCorresponding Author
Mei Wei
Department of Materials Science and Engineering, University of Connecticut, Storrs, Connecticut, USA
Institute of Materials Science, University of Connecticut, Storrs, Connecticut, USA
Department of Mechanical Engineering, Ohio University, Athens, Ohio, USA
Correspondence
Mei Wei, Dean's office, Russ College of Engineering and Technology, Ohio University, Athens, OH 45701.
Email: [email protected]
Search for more papers by this authorLe Yu
Department of Materials Science and Engineering, University of Connecticut, Storrs, Connecticut, USA
Department of Chemical and Biomolecular Engineering, Ohio University, Athens, Ohio, USA
Search for more papers by this authorTomas M. Silva Santisteban
Department of Materials & Structure Analysis, Thermo Fisher Scientific, Houston, Texas, USA
Search for more papers by this authorQinqing Liu
Department of Computer Science and Engineering, University of Connecticut, Storrs, Connecticut, USA
Search for more papers by this authorChangmin Hu
Institute of Materials Science, University of Connecticut, Storrs, Connecticut, USA
Search for more papers by this authorJinbo Bi
Department of Computer Science and Engineering, University of Connecticut, Storrs, Connecticut, USA
Search for more papers by this authorCorresponding Author
Mei Wei
Department of Materials Science and Engineering, University of Connecticut, Storrs, Connecticut, USA
Institute of Materials Science, University of Connecticut, Storrs, Connecticut, USA
Department of Mechanical Engineering, Ohio University, Athens, Ohio, USA
Correspondence
Mei Wei, Dean's office, Russ College of Engineering and Technology, Ohio University, Athens, OH 45701.
Email: [email protected]
Search for more papers by this authorFunding information: General Electric; Thermo Fisher Scientific
Abstract
Surface modification techniques are often used to enhance the properties of Ti-based materials as hard-tissue replacements. While the microstructure of the coating and the quality of the interface between the substrate and coating are essential to evaluate the reliability and applicability of the surface modification. In this study, both a hydroxyapatite (HA) coating and a collagen-hydroxyapatite (Col-HA) composite coating were deposited onto a Ti-6Al-4V substrate using a biomimetic coating process. Importantly, a gradient cross-sectional structure with a porous coating toward the surface, while a dense layer adjacent to the interface between the coating and substrate was observed in three-dimensional (3D) from both the HA and Col-HA coatings via a dual-beam focused ion beam-scanning electron microscope (FIB-SEM). Moreover, the pore distributions within the entire coatings were reconstructed in 3D using Avizo, and the pores size distributions along the coating depth were calculated using RStudio. By evaluating the mechanical property and biocompatibility of these materials and closely observing the cross-sectional cell-coating-substrate interfaces using FIB-SEM, it was revealed that the porous surface created by both coatings well supports osteoblast cell adhesion while the dense inner layer facilitates a good bonding between the coating and the substrate. Although the mechanical property of the coating decreased with the addition of collagen, it is still strong enough for implant handling and the biocompatibility was promoted.
CONFLICT OF INTERESTS
Dr Wei has an ownership interest in OrteoPoniX LLC, which is developing and commercializing biomaterials for orthopedic applications.
Supporting Information
| Filename | Description |
|---|---|
| jbma37046-sup-0001-Supinfo.docxWord 2007 document , 1.4 MB | Scheme S1. Illustration of HA and Col-HA coating deposition process. SLA refers to sandblasting with large grit and acid etching. FIGURE S1. SEM cross-section sample preparation sequence by GFIB: (A) Surface of HA coating; (B) A Pt protective layer coated on the surface of HA coating at the area of interest; (C) A wedge was milled along with the Pt layer using RCS pattern; (D) SEM image of cross-section after cleaning using both CCS and Rec patterns. FIGURE S2. Steps of serial imaging collection using Auto Slice and View in GFIB: (A) Ion beam (IB) view of fiducial pattern; (B) Electron beam (EB) and (C) ion beam views of fiducial pattern and area of interest (AOI); (D) EB view of AOI (Red arrow denotes the front wall of the coating). FIGURE S3. Illustration of directions of coordinate axis used in Avizo and RSudio. FIGURE S4. XRD patterns and FTIR spectra of HA and Col-HA coatings. FIGURE S5. Bonding strength of HA and Col-HA coatings on a Ti-6Al-4V substrate. FIGURE S6. SEM images of MC3T3 cells seeded on the HA (A) and the Col-HA (B) coatings for 7 days. The insets show the cell morphology at a higher magnification. TABLE S1. Ion concentration (mM) of human blood plasma and m-SBF. |
| jbma37046-sup-0002-VideoS1.mp4MPEG-4 video, 13.7 MB | Video S1. Pore Segmentation Animation-HA coating. |
| jbma37046-sup-0003-VideoS2.mp4MPEG-4 video, 12.7 MB | Video S2. Pore Segmentation Animation-Col-HA coating. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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