Regulation of cell arrangement using a novel composite micropattern
Xiaoyi Liu
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191 People's Republic of China
State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050 People's Republic of China
These authors contributed equally to this work.
Search for more papers by this authorYaoping Liu
Institute of Microelectronics, Peking University, Beijing, 100871 People's Republic of China
These authors contributed equally to this work.
Search for more papers by this authorFeng Zhao
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191 People's Republic of China
State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050 People's Republic of China
Search for more papers by this authorTingting Hun
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191 People's Republic of China
State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050 People's Republic of China
Search for more papers by this authorShan Li
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191 People's Republic of China
State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050 People's Republic of China
Search for more papers by this authorYuguang Wang
Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, 100083 People's Republic of China
Search for more papers by this authorWeijie Sun
Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, 100083 People's Republic of China
Search for more papers by this authorWei Wang
Institute of Microelectronics, Peking University, Beijing, 100871 People's Republic of China
National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Beijing, 100871 China
Innovation Center for Micro-Nano-electronics and Integrated System, Beijing, 100871 China
Search for more papers by this authorCorresponding Author
Yan Sun
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191 People's Republic of China
State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050 People's Republic of China
Correspondence to: Y. Fan; e-mail: [email protected] and Y. Sun; e-mail: [email protected]Search for more papers by this authorCorresponding Author
Yubo Fan
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191 People's Republic of China
National Research Center for Rehabilitation Technical Aids, Beijing, 100176 People's Republic of China
Correspondence to: Y. Fan; e-mail: [email protected] and Y. Sun; e-mail: [email protected]Search for more papers by this authorXiaoyi Liu
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191 People's Republic of China
State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050 People's Republic of China
These authors contributed equally to this work.
Search for more papers by this authorYaoping Liu
Institute of Microelectronics, Peking University, Beijing, 100871 People's Republic of China
These authors contributed equally to this work.
Search for more papers by this authorFeng Zhao
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191 People's Republic of China
State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050 People's Republic of China
Search for more papers by this authorTingting Hun
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191 People's Republic of China
State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050 People's Republic of China
Search for more papers by this authorShan Li
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191 People's Republic of China
State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050 People's Republic of China
Search for more papers by this authorYuguang Wang
Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, 100083 People's Republic of China
Search for more papers by this authorWeijie Sun
Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, 100083 People's Republic of China
Search for more papers by this authorWei Wang
Institute of Microelectronics, Peking University, Beijing, 100871 People's Republic of China
National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Beijing, 100871 China
Innovation Center for Micro-Nano-electronics and Integrated System, Beijing, 100871 China
Search for more papers by this authorCorresponding Author
Yan Sun
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191 People's Republic of China
State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050 People's Republic of China
Correspondence to: Y. Fan; e-mail: [email protected] and Y. Sun; e-mail: [email protected]Search for more papers by this authorCorresponding Author
Yubo Fan
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191 People's Republic of China
National Research Center for Rehabilitation Technical Aids, Beijing, 100176 People's Republic of China
Correspondence to: Y. Fan; e-mail: [email protected] and Y. Sun; e-mail: [email protected]Search for more papers by this authorAbstract
Micropatterning technique has been used to control single cell geometry in many researches, however, this is no report that it is used to control multicelluar geometry, which not only control single cell geometry but also organize those cells by a certain pattern. In this work, a composite protein micropattern is developed to control both cell shape and cell location simultaneously. The composite micropattern consists of a central circle 15 μm in diameter for single-cell capture, surrounded by small, square arrays (3 μm × 3 μm) for cell spreading. This is surrounded by a border 2 μm wide for restricting cell edges. The composite pattern results in two-cell and three-cell capture efficiencies of 32.1% ± 1.94% and 24.2% ± 2.89%, respectively, representing an 8.52% and 9.58% increase, respectively, over rates of original patterns. Fluorescent imaging of cytoskeleton alignment demonstrates that actin is gradually aligned parallel to the direction of the entire pattern arrangement, rather than to that of a single pattern. This indicates that cell arrangement is also an important factor in determining cell physiology. This composite micropattern could be a potential method to precisely control multi-cells for cell junctions, cell interactions, cell signal transduction, and eventually for tissue rebuilding study. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3093–3101, 2017.
Supporting Information
Additional Supporting Information may be found in the online version of this article.
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| jbma36157-sup-0001-suppinfo001.tif9.3 MB | Supporting Information Figure 1 |
| jbma36157-sup-0002-suppinfo002.tif17.7 MB | Supporting Information Figure 2 |
| jbma36157-sup-0003-suppinfo003.tif3.3 MB | Supporting Information Figure 3 |
| jbma36157-sup-0004-suppinfo004.tif19.2 MB | Supporting Information Figure 4 |
| jbma36157-sup-0005-suppinfo005.tif28.1 MB | Supporting Information Figure 5 |
| jbma36157-sup-0006-suppinfo006.tif6.8 MB | Supporting Information Figure 6 |
| jbma36157-sup-0007-suppinfo007.tif15.5 MB | Supporting Information Figure 7 |
| jbma36157-sup-0008-suppinfo008.doc21.3 KB | Supporting Information |
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