Original Article
Nanoporous metals for biodegradable implants: Initial bone mesenchymal stem cell adhesion and degradation behavior
Michael Heiden,
Sabrina Huang,
Eric Nauman,
David Johnson,
Lia Stanciu,
Corresponding Author
Michael Heiden
Department of Materials Engineering, School of Materials Science and Engineering, Purdue University, West Lafayette, Indiana
Correspondence to: M. Heiden; e-mail: [email protected]Search for more papers by this authorSabrina Huang
Department of Materials Engineering, School of Materials Science and Engineering, Purdue University, West Lafayette, Indiana
Search for more papers by this authorEric Nauman
Department of Biomedical Engineering, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana
Department of Mechanical Engineering, School of Mechanical Engineering, Purdue University, West Lafayette, Indiana
Department of Basic Medical Sciences, Purdue University, West Lafayette, Indiana
Search for more papers by this authorDavid Johnson
Department of Materials Engineering, School of Materials Science and Engineering, Purdue University, West Lafayette, Indiana
Search for more papers by this authorLia Stanciu
Department of Materials Engineering, School of Materials Science and Engineering, Purdue University, West Lafayette, Indiana
Search for more papers by this authorMichael Heiden,
Sabrina Huang,
Eric Nauman,
David Johnson,
Lia Stanciu,
Corresponding Author
Michael Heiden
Department of Materials Engineering, School of Materials Science and Engineering, Purdue University, West Lafayette, Indiana
Correspondence to: M. Heiden; e-mail: [email protected]Search for more papers by this authorSabrina Huang
Department of Materials Engineering, School of Materials Science and Engineering, Purdue University, West Lafayette, Indiana
Search for more papers by this authorEric Nauman
Department of Biomedical Engineering, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana
Department of Mechanical Engineering, School of Mechanical Engineering, Purdue University, West Lafayette, Indiana
Department of Basic Medical Sciences, Purdue University, West Lafayette, Indiana
Search for more papers by this authorDavid Johnson
Department of Materials Engineering, School of Materials Science and Engineering, Purdue University, West Lafayette, Indiana
Search for more papers by this authorLia Stanciu
Department of Materials Engineering, School of Materials Science and Engineering, Purdue University, West Lafayette, Indiana
Search for more papers by this authorAbstract
Nanostructured Fe-Mn and Fe-Mn-Zn metal scaffolds were generated through a well-controlled selective leaching process in order to fulfill the growing demand for adjustable degradation rates and improved cellular response of resorbable materials. Mouse bone marrow mesenchymal stem cells (D1 ORL UVA) were seeded onto eleven, carefully chosen nanoporous surfaces for 24 h in vitro. Using a combination of fluorescence microscopy, scanning electron microscopy (SEM), and an MTS assay, it was discovered that scaffolds with nanoscale roughened surfaces had increased cell attachment by up to 123% compared to polished smooth Fe-Mn surfaces. Significant cell spreading and construction of cell multilayers were also apparent after 24 h, suggesting better adhesion. Additionally, static electrochemical polarization experiments revealed an improvement of up to 26% in the actual rate of biodegradation for Fe-Mn surface-modified materials. However, any residual concentration of zinc after leaching was shown to slightly increase corrosion resistance. The results demonstrate that selectively leached, nanostructured Fe-Mn surfaces have the potential of being tailored to a diverse set of transient implant scenarios, while also effectively boosting overall biocompatibility, initial cell attachment, and degradation rate. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1747–1758, 2016.
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