A hybrid microfluidic system for regulation of neural differentiation in induced pluripotent stem cells
Zahra Hesari
Deparmentof Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Search for more papers by this authorMassoud Soleimani
Department of Hematology and Blood Banking, Faculty of Medicine, Tarbiat Modaress University, Tehran, Iran
Search for more papers by this authorFatemeh Atyabi
Deparmentof Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Search for more papers by this authorMeysam Sharifdini
Department of Medical Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
Search for more papers by this authorSamad Nadri
Medical Biotechnology and Nanotechnology Department, Faculty of Medicine, Zanjan University of Medical Science, Zanjan, Iran
Search for more papers by this authorMajid Ebrahimi Warkiani
School of Mechanical and Manufacturing Engineering, Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia
Search for more papers by this authorCorresponding Author
Mehrak Zare
Skin and Stemcell Research Center, Tehran University of Medical Sciences, Tehran, Iran
Correspondence to: R. Dinarvand; e-mail: [email protected]Search for more papers by this authorCorresponding Author
Rassoul Dinarvand
Deparmentof Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Correspondence to: R. Dinarvand; e-mail: [email protected]Search for more papers by this authorZahra Hesari
Deparmentof Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Search for more papers by this authorMassoud Soleimani
Department of Hematology and Blood Banking, Faculty of Medicine, Tarbiat Modaress University, Tehran, Iran
Search for more papers by this authorFatemeh Atyabi
Deparmentof Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Search for more papers by this authorMeysam Sharifdini
Department of Medical Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
Search for more papers by this authorSamad Nadri
Medical Biotechnology and Nanotechnology Department, Faculty of Medicine, Zanjan University of Medical Science, Zanjan, Iran
Search for more papers by this authorMajid Ebrahimi Warkiani
School of Mechanical and Manufacturing Engineering, Australian Centre for NanoMedicine, University of New South Wales, Sydney, Australia
Search for more papers by this authorCorresponding Author
Mehrak Zare
Skin and Stemcell Research Center, Tehran University of Medical Sciences, Tehran, Iran
Correspondence to: R. Dinarvand; e-mail: [email protected]Search for more papers by this authorCorresponding Author
Rassoul Dinarvand
Deparmentof Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Correspondence to: R. Dinarvand; e-mail: [email protected]Search for more papers by this authorAbstract
Controlling cellular orientation, proliferation, and differentiation is valuable in designing organ replacements and directing tissue regeneration. In the present study, we developed a hybrid microfluidic system to produce a dynamic microenvironment by placing aligned PDMS microgrooves on surface of biodegradable polymers as physical guidance cues for controlling the neural differentiation of human induced pluripotent stem cells (hiPSCs). The neuronal differentiation capacity of cultured hiPSCs in the microfluidic system and other control groups was investigated using quantitative real time PCR (qPCR) and immunocytochemistry. The functionally of differentiated hiPSCs inside hybrid system's scaffolds was also evaluated on the rat hemisected spinal cord in acute phase. Implanted cell's fate was examined using tissue freeze section and the functional recovery was evaluated according to the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale. Our results confirmed the differentiation of hiPSCs to neuronal cells on the microfluidic device where the expression of neuronal-specific genes was significantly higher compared to those cultured on the other systems such as plain tissue culture dishes and scaffolds without fluidic channels. Although survival and integration of implanted hiPSCs did not lead to a significant functional recovery, we believe that combination of fluidic channels with nanofiber scaffolds provides a great microenvironment for neural tissue engineering, and can be used as a powerful tool for in situ monitoring of differentiation potential of various kinds of stem cells. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1534–1543, 2016.
Supporting Information
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