The efficacy of polycaprolactone/hydroxyapatite scaffold in combination with mesenchymal stem cells for bone tissue engineering
Corresponding Author
Boontharika Chuenjitkuntaworn
Department of Oral Biology, Faculty of Dentistry, Naresuan University, Phitsanulok, 65000 Thailand
Correspondence to: B. Chuenjitkuntaworn; e-mail: [email protected]Search for more papers by this authorThanaphum Osathanon
Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330 Thailand
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Pathumwan 10330 Thailand
Search for more papers by this authorNunthawan Nowwarote
Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330 Thailand
Search for more papers by this authorPitt Supaphol
The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok, Pathumwan 10330 Thailand
Search for more papers by this authorPrasit Pavasant
Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330 Thailand
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Pathumwan 10330 Thailand
Search for more papers by this authorCorresponding Author
Boontharika Chuenjitkuntaworn
Department of Oral Biology, Faculty of Dentistry, Naresuan University, Phitsanulok, 65000 Thailand
Correspondence to: B. Chuenjitkuntaworn; e-mail: [email protected]Search for more papers by this authorThanaphum Osathanon
Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330 Thailand
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Pathumwan 10330 Thailand
Search for more papers by this authorNunthawan Nowwarote
Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330 Thailand
Search for more papers by this authorPitt Supaphol
The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok, Pathumwan 10330 Thailand
Search for more papers by this authorPrasit Pavasant
Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330 Thailand
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Pathumwan 10330 Thailand
Search for more papers by this authorAbstract
Major drawbacks of using an autograft are the possibilities of insufficient bony source and patient's morbidity after operation. Bone tissue engineering technology, therefore, has been applied for repairing bony defects. Previous study showed that a novel fabricated 3D-Polycaprolactone/Hydroxyapatite (PCL/HAp) scaffold possessed a good biocompatibility for bone cells. This study aimed to determine the ability of PCL/HAp for supporting cell growth, gene expression, and osteogenic differentiation in three types of mesenchymal stem cells, including bone marrow-derived mesenchymal stem cells (BMSCs), dental pulp stem cells (DPSCs), and adiposed-derived mesenchymal stem cells (ADSCs). These were assessed by cell viability assay (MTT), reverse-transcription polymerase chain reaction (RT-PCR) analysis, alkaline phosphatase activity, and osteogenic differentiation by alizarin red-S staining. The results showed that PCL/HAp scaffold could support growth of all three types of mesenchymal stem cells. In addition, DPSCs with PCL/HAp showed the highest level of calcium deposition compared to other groups. In conclusion, DPSCs exhibited a better compatibility with these scaffolds compared to BMSCs and ADSCs. However, the PCL/HAp could be a good candidate scaffold for all tested mesenchymal stem cells in bone tissue engineering. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: A: 264–271, 2016.
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