Tissue performance of bladder following stretched electrospun silk fibroin matrix and bladder acellular matrix implantation in a rabbit model
Jian-Wen Huang
Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233 China
Jian-Wen Huang and Yue-Min Xu contributed equally to this work.
Search for more papers by this authorYue-Min Xu
Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233 China
Jian-Wen Huang and Yue-Min Xu contributed equally to this work.
Search for more papers by this authorZhao-Bo Li
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620 China
Search for more papers by this authorSean V. Murphy
Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Blvd, Winston Salem, North Carolina, 27157
Search for more papers by this authorWeixin Zhao
Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Blvd, Winston Salem, North Carolina, 27157
Search for more papers by this authorQiang-Qiang Liu
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620 China
Search for more papers by this authorWei-Dong Zhu
Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233 China
Search for more papers by this authorQiang Fu
Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233 China
Search for more papers by this authorCorresponding Author
Yao-Peng Zhang
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620 China
Correspondence to: L.-J. Song; e-mail: [email protected] and Y.-P. Zhang; e-mail: [email protected]Search for more papers by this authorCorresponding Author
Lu-Jie Song
Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233 China
Correspondence to: L.-J. Song; e-mail: [email protected] and Y.-P. Zhang; e-mail: [email protected]Search for more papers by this authorJian-Wen Huang
Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233 China
Jian-Wen Huang and Yue-Min Xu contributed equally to this work.
Search for more papers by this authorYue-Min Xu
Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233 China
Jian-Wen Huang and Yue-Min Xu contributed equally to this work.
Search for more papers by this authorZhao-Bo Li
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620 China
Search for more papers by this authorSean V. Murphy
Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Blvd, Winston Salem, North Carolina, 27157
Search for more papers by this authorWeixin Zhao
Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Blvd, Winston Salem, North Carolina, 27157
Search for more papers by this authorQiang-Qiang Liu
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620 China
Search for more papers by this authorWei-Dong Zhu
Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233 China
Search for more papers by this authorQiang Fu
Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233 China
Search for more papers by this authorCorresponding Author
Yao-Peng Zhang
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620 China
Correspondence to: L.-J. Song; e-mail: [email protected] and Y.-P. Zhang; e-mail: [email protected]Search for more papers by this authorCorresponding Author
Lu-Jie Song
Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233 China
Correspondence to: L.-J. Song; e-mail: [email protected] and Y.-P. Zhang; e-mail: [email protected]Search for more papers by this authorAbstract
The goal of this study was to investigate the tissue performance of bladder following stretched electrospun silk fibroin matrix (SESFM) implantation compared with bladder acellular matrix (BAM). We compared SESFM with BAM based on porosity and pore size. Scaffolds were separately transplanted into opposite walls of the bladder of 30 rabbits after stripping the bladder mucosa and smooth muscle (1.5 × 2.0 cm2). Gross anatomical observation, histological analysis and muscle contractility studies were performed at 2, 4, and 8 weeks post-op. SESFM has higher porosity and larger pore size compared with BAM (p < 0.05). At 2 weeks, the presence of vesical calculus was evident in 7/10 rabbits. Histological analysis showed that SESFM and BAM promoted similar degree of urothelium regeneration (p > 0.05). However, SESFM promoted a higher degree of smooth muscle and vessel regeneration compared to BAM (p < 0.05). In addition, muscle strips supported by SESFM displayed higher contractile responses to carbachol, KCl, and phenylephrine compared with BAM. At 8 weeks, both matrices elicited similar mild acute and chronic inflammatory reactions. Our results demonstrated that SESFM has greater ability to promote bladder tissue regeneration with structural and functional properties compared to BAM, and with similar biocompatibility. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 9–16, 2016.
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