Engineering and application of collagen-binding fibroblast growth factor 2 for sustained release
Eunyi Jeon
Department of Biochemistry, Inha University School of Medicine, Incheon, 400-712 Korea
These authors contributed equally to this work.
Search for more papers by this authorYe-Rang Yun
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, South Korea
Department of Nanobiomedical Science and WCU Research Center, Dankook University Graduate School, Cheonan, 330-714 Korea
These authors contributed equally to this work.
Search for more papers by this authorCorresponding Author
Hae-Won Kim
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, South Korea
Department of Nanobiomedical Science and WCU Research Center, Dankook University Graduate School, Cheonan, 330-714 Korea
Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan, 330-714 Korea
Correspondence to: J.-H. Jang; e-mail: [email protected] or H.-W. Kim; e-mail: [email protected]Search for more papers by this authorCorresponding Author
Jun-Hyeog Jang
Department of Biochemistry, Inha University School of Medicine, Incheon, 400-712 Korea
Correspondence to: J.-H. Jang; e-mail: [email protected] or H.-W. Kim; e-mail: [email protected]Search for more papers by this authorEunyi Jeon
Department of Biochemistry, Inha University School of Medicine, Incheon, 400-712 Korea
These authors contributed equally to this work.
Search for more papers by this authorYe-Rang Yun
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, South Korea
Department of Nanobiomedical Science and WCU Research Center, Dankook University Graduate School, Cheonan, 330-714 Korea
These authors contributed equally to this work.
Search for more papers by this authorCorresponding Author
Hae-Won Kim
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, South Korea
Department of Nanobiomedical Science and WCU Research Center, Dankook University Graduate School, Cheonan, 330-714 Korea
Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan, 330-714 Korea
Correspondence to: J.-H. Jang; e-mail: [email protected] or H.-W. Kim; e-mail: [email protected]Search for more papers by this authorCorresponding Author
Jun-Hyeog Jang
Department of Biochemistry, Inha University School of Medicine, Incheon, 400-712 Korea
Correspondence to: J.-H. Jang; e-mail: [email protected] or H.-W. Kim; e-mail: [email protected]Search for more papers by this authorAbstract
The sustained release of growth factors plays a critical role in therapeutic applications because of the instability of these factors in the body. Here, we designed a fibroblast growth factor 2 (FGF2) fused with a collagen-binding domain (rhCBD-FGF2) for collagen-based sustained release of FGF2.The release profile of rhCBD-FGF2 showed sustained release from collagen matrices. Further, rhCBD-FGF2 also stimulated adhesion of the MC3T3-E1 cells to the collagen matrices. In addition, rhCBD-FGF2 increased the cell proliferation activity at 3 and 5 days in the MC3T3-E1 cells attached to the collagen matrices compared to that in the control. Further, rhCBD-FGF2 significantly induced the osteogenic differentiation of MC3T3-E1 cells on collagen matrices by up-regulating the alkaline phosphatase activity at 7 days. These osteogenic differentiation activities were confirmed in gene expression of MC3T3-E1 cell. Taken together, rhCBD-FGF2 could specifically bind with collagen matrices, which indicates important advancements in bone tissue engineering. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1–7, 2014.
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