Synthesis of nanostructured porous silica coatings on titanium and their cell adhesive and osteogenic differentiation properties
Débora Inzunza
Departamento de Ciencias Básicas, Laboratorio de Nanobiomateriales, Facultad de Odontología, Universidad de Chile, Sergio Livingstone 943, Independencia, Santiago, Chile
Search for more papers by this authorCorresponding Author
Cristian Covarrubias
Departamento de Ciencias Básicas, Laboratorio de Nanobiomateriales, Facultad de Odontología, Universidad de Chile, Sergio Livingstone 943, Independencia, Santiago, Chile
Correspondence to: C. Covarrubias; e-mail: [email protected]Search for more papers by this authorAlfredo Von Marttens
Departamento de Prótesis, Facultad de Odontología, Universidad de Chile, Chile
Search for more papers by this authorYerko Leighton
Departamento de Prótesis, Facultad de Odontología, Universidad de Chile, Chile
Search for more papers by this authorJuan Carlos Carvajal
Departamento de Prótesis, Facultad de Odontología, Universidad de Chile, Chile
Search for more papers by this authorFrancisco Valenzuela
Departamento de Ciencias Básicas, Laboratorio de Nanobiomateriales, Facultad de Odontología, Universidad de Chile, Sergio Livingstone 943, Independencia, Santiago, Chile
Search for more papers by this authorMario Díaz-Dosque
Departamento de Ciencias Básicas, Laboratorio de Nanobiomateriales, Facultad de Odontología, Universidad de Chile, Sergio Livingstone 943, Independencia, Santiago, Chile
Search for more papers by this authorNicolás Méndez
Laboratorio de Biología Celular, INTA, Universidad de Chile, Chile
Search for more papers by this authorConstanza Martínez
Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile
Search for more papers by this authorAna María Pino
Laboratorio de Biología Celular, INTA, Universidad de Chile, Chile
Search for more papers by this authorJuan Pablo Rodríguez
Laboratorio de Biología Celular, INTA, Universidad de Chile, Chile
Search for more papers by this authorMónica Cáceres
Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile
Search for more papers by this authorPatricio Smith
Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile
Search for more papers by this authorDébora Inzunza
Departamento de Ciencias Básicas, Laboratorio de Nanobiomateriales, Facultad de Odontología, Universidad de Chile, Sergio Livingstone 943, Independencia, Santiago, Chile
Search for more papers by this authorCorresponding Author
Cristian Covarrubias
Departamento de Ciencias Básicas, Laboratorio de Nanobiomateriales, Facultad de Odontología, Universidad de Chile, Sergio Livingstone 943, Independencia, Santiago, Chile
Correspondence to: C. Covarrubias; e-mail: [email protected]Search for more papers by this authorAlfredo Von Marttens
Departamento de Prótesis, Facultad de Odontología, Universidad de Chile, Chile
Search for more papers by this authorYerko Leighton
Departamento de Prótesis, Facultad de Odontología, Universidad de Chile, Chile
Search for more papers by this authorJuan Carlos Carvajal
Departamento de Prótesis, Facultad de Odontología, Universidad de Chile, Chile
Search for more papers by this authorFrancisco Valenzuela
Departamento de Ciencias Básicas, Laboratorio de Nanobiomateriales, Facultad de Odontología, Universidad de Chile, Sergio Livingstone 943, Independencia, Santiago, Chile
Search for more papers by this authorMario Díaz-Dosque
Departamento de Ciencias Básicas, Laboratorio de Nanobiomateriales, Facultad de Odontología, Universidad de Chile, Sergio Livingstone 943, Independencia, Santiago, Chile
Search for more papers by this authorNicolás Méndez
Laboratorio de Biología Celular, INTA, Universidad de Chile, Chile
Search for more papers by this authorConstanza Martínez
Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile
Search for more papers by this authorAna María Pino
Laboratorio de Biología Celular, INTA, Universidad de Chile, Chile
Search for more papers by this authorJuan Pablo Rodríguez
Laboratorio de Biología Celular, INTA, Universidad de Chile, Chile
Search for more papers by this authorMónica Cáceres
Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile
Search for more papers by this authorPatricio Smith
Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile
Search for more papers by this authorAbstract
Nanostructured porous silica coatings were synthesized on titanium by the combined sol–gel and evaporation-induced self-assembly process. The silica-coating structures were characterized by X-ray diffraction, transmission electron microscopy, scanning electron microscopy, and nitrogen sorptometry. The effect of the nanoporous surface on apatite formation in simulated body fluid, protein adsorption, osteoblast cell adhesion behavior, and osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) is reported. Silica coatings with highly ordered sub-10 nm porosity accelerate early osteoblast adhesive response, a favorable cell response that is attributed to an indirect effect due to the high protein adsorption observed on the large-specific surface area of the nanoporous coating but is also probably due to direct mechanical stimulus from the nanostructured topography. The nanoporous silica coatings, particularly those doped with calcium and phosphate, also promote the osteogenic differentiation of hBMSCs with spontaneous mineral nodule formation in basal conditions. The bioactive surface properties exhibited by the nanostructured porous silica coatings make these materials a promising alternative to improve the osseointegration properties of titanium dental implants and could have future impact on the nanoscale design of implant surfaces. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 37–48, 2014.
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