Patterned macroarray plates in comparison of bacterial adhesion inhibition of tantalum, titanium, and chromium compared with diamond-like carbon
Jaakko Levon
Department of Anatomy, Institute of Biomedicine, University of Helsinki, Haartmaninkatu 8, FI-00014 HY, Finland
Department of Medicine/Invärtes Medicin, Helsinki University Central Hospital, Biomedicum Helsinki Bldg, Haartmaninkatu 8, FI-00029 HUS, Finland
These authors contributed equally to this work.
Search for more papers by this authorKatja Myllymaa
Department of Physics, University of Kuopio, Yliopistonranta 1 E, FI-70211 Kuopio, Finland
These authors contributed equally to this work.
Search for more papers by this authorVesa-Petteri Kouri
Department of Anatomy, Institute of Biomedicine, University of Helsinki, Haartmaninkatu 8, FI-00014 HY, Finland
Department of Medicine/Invärtes Medicin, Helsinki University Central Hospital, Biomedicum Helsinki Bldg, Haartmaninkatu 8, FI-00029 HUS, Finland
Search for more papers by this authorRiina Rautemaa
Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, FI-00014 HY, Finland
Department of Oral and Maxillofacial Diseases, Helsinki University Central Hospital, Kasarminkatu 11-13, FIN-00029 HUS, Finland
Microbiology Unit, Laboratory Diagnostics, Helsinki University Central Hospital, Haartmaninkatu 3, FIN-00029 HUS, Finland
Search for more papers by this authorTeemu Kinnari
Department of Otorhinolaryngology, Helsinki University Central Hospital, Haartmaninkatu 4, FIN-00029 HUS, Finland
Search for more papers by this authorSami Myllymaa
Department of Physics, University of Kuopio, Yliopistonranta 1 E, FI-70211 Kuopio, Finland
Search for more papers by this authorCorresponding Author
Yrjö T. Konttinen
Department of Anatomy, Institute of Biomedicine, University of Helsinki, Haartmaninkatu 8, FI-00014 HY, Finland
Department of Medicine/Invärtes Medicin, Helsinki University Central Hospital, Biomedicum Helsinki Bldg, Haartmaninkatu 8, FI-00029 HUS, Finland
ORTON Orthopaedic Hospital of the Invalid Foundation, Tenholantie 10, FIN-00280 Helsinki, Finland
COXA Hospital for Joint Replacement, Biokatu 6b, FIN-33520 Tampere, Finland
Department of Anatomy, Institute of Biomedicine, University of Helsinki, Haartmaninkatu 8, FI-00014 HY, FinlandSearch for more papers by this authorReijo Lappalainen
Department of Physics, University of Kuopio, Yliopistonranta 1 E, FI-70211 Kuopio, Finland
Search for more papers by this authorJaakko Levon
Department of Anatomy, Institute of Biomedicine, University of Helsinki, Haartmaninkatu 8, FI-00014 HY, Finland
Department of Medicine/Invärtes Medicin, Helsinki University Central Hospital, Biomedicum Helsinki Bldg, Haartmaninkatu 8, FI-00029 HUS, Finland
These authors contributed equally to this work.
Search for more papers by this authorKatja Myllymaa
Department of Physics, University of Kuopio, Yliopistonranta 1 E, FI-70211 Kuopio, Finland
These authors contributed equally to this work.
Search for more papers by this authorVesa-Petteri Kouri
Department of Anatomy, Institute of Biomedicine, University of Helsinki, Haartmaninkatu 8, FI-00014 HY, Finland
Department of Medicine/Invärtes Medicin, Helsinki University Central Hospital, Biomedicum Helsinki Bldg, Haartmaninkatu 8, FI-00029 HUS, Finland
Search for more papers by this authorRiina Rautemaa
Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, FI-00014 HY, Finland
Department of Oral and Maxillofacial Diseases, Helsinki University Central Hospital, Kasarminkatu 11-13, FIN-00029 HUS, Finland
Microbiology Unit, Laboratory Diagnostics, Helsinki University Central Hospital, Haartmaninkatu 3, FIN-00029 HUS, Finland
Search for more papers by this authorTeemu Kinnari
Department of Otorhinolaryngology, Helsinki University Central Hospital, Haartmaninkatu 4, FIN-00029 HUS, Finland
Search for more papers by this authorSami Myllymaa
Department of Physics, University of Kuopio, Yliopistonranta 1 E, FI-70211 Kuopio, Finland
Search for more papers by this authorCorresponding Author
Yrjö T. Konttinen
Department of Anatomy, Institute of Biomedicine, University of Helsinki, Haartmaninkatu 8, FI-00014 HY, Finland
Department of Medicine/Invärtes Medicin, Helsinki University Central Hospital, Biomedicum Helsinki Bldg, Haartmaninkatu 8, FI-00029 HUS, Finland
ORTON Orthopaedic Hospital of the Invalid Foundation, Tenholantie 10, FIN-00280 Helsinki, Finland
COXA Hospital for Joint Replacement, Biokatu 6b, FIN-33520 Tampere, Finland
Department of Anatomy, Institute of Biomedicine, University of Helsinki, Haartmaninkatu 8, FI-00014 HY, FinlandSearch for more papers by this authorReijo Lappalainen
Department of Physics, University of Kuopio, Yliopistonranta 1 E, FI-70211 Kuopio, Finland
Search for more papers by this authorAbstract
Staphylococcus aureus device-related infection is a common complication in implantology. Bacterial adhesion on implant surfaces is the initial step in the infective process. The aim was to develop a method suitable for quantitative bacterial adherence studies and to test a new diamond-like carbon (DLC) coating against commonly used metallic biomaterials with regards to Staphylococcus aureus adhesion. Patterned silicon chips with spots of tantalum, titanium, chromium, and DLC were produced using ultraviolet lithography and physical vapor deposition. These patterned chips were used as such or glued to array plates, pretreated with serum and exposed to S. aureus (S-15981) for 90 min, followed by acridine orange staining and fluorescence microscopy. An adhesion index showed that the ranking order of the biomaterials was titanium, tantalum, chromium, and DLC, with the DLC being clearly most resistant against colonization with S. aureus. Micropatterned surfaces are useful for quantitative comparison of bacterial adherence on different biomaterials. In the presence of serum, DLC is superior in its ability to resist adhesion and colonization by S. aureus compared with the commonly used biomaterial metals tantalum, titanium, and chromium. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
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