Resistance to apoptosis of HCW-2 cells can be overcome by curcumin- or vincristine-induced mitotic catastrophe
Adriana Magalska
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland
Search for more papers by this authorMalgorzata Sliwinska
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland
Search for more papers by this authorJoanna Szczepanowska
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland
Search for more papers by this authorStefano Salvioli
Department of Experimental Pathology, University of Bologna, Bologna, Italy
Centro Interdipartimentale “L. Galvani”, University of Bologna, Bologna, Italy
Search for more papers by this authorClaudio Franceschi
Department of Experimental Pathology, University of Bologna, Bologna, Italy
Centro Interdipartimentale “L. Galvani”, University of Bologna, Bologna, Italy
Department of Gerontology, INRCA, Ancona, Italy
Search for more papers by this authorCorresponding Author
Ewa Sikora
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland
Fax: +4822-225342.
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, 3 Pasteura St., Warsaw 02-093, PolandSearch for more papers by this authorAdriana Magalska
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland
Search for more papers by this authorMalgorzata Sliwinska
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland
Search for more papers by this authorJoanna Szczepanowska
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland
Search for more papers by this authorStefano Salvioli
Department of Experimental Pathology, University of Bologna, Bologna, Italy
Centro Interdipartimentale “L. Galvani”, University of Bologna, Bologna, Italy
Search for more papers by this authorClaudio Franceschi
Department of Experimental Pathology, University of Bologna, Bologna, Italy
Centro Interdipartimentale “L. Galvani”, University of Bologna, Bologna, Italy
Department of Gerontology, INRCA, Ancona, Italy
Search for more papers by this authorCorresponding Author
Ewa Sikora
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland
Fax: +4822-225342.
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, 3 Pasteura St., Warsaw 02-093, PolandSearch for more papers by this authorAbstract
The term mitotic catastrophe has recently become widely used to describe a form of death affecting many cancer cells, which, because of severe DNA or mitotic spindle damage, are not able to bypass mitosis. We show here that cells of the HL-60-derived HCW-2 line highly resistant to apoptosis, upon treatment with curcumin or vincristine, undergo mitotic catastrophe that is finalized by caspase 3 activation and oligonucleosomal DNA degradation. Curcumin is a natural dye, derived from Curcuma longa that has been shown to induce cell death in many cancer cells. Both treatments decrease cell proliferation and cell survival, arrest cells in G2/M phase of cell cycle and induce morphological changes characterized by cell enlargement and micronucleation. “Catastrophic” cells comprise a separate subpopulation with less than 4C DNA, as evidenced by flow and scanning cytometry. This subpopulation is MPM-2 positive. Thymidine block increased the number of cell arrested in the G2/M phase of cell cycle and curcumin effectiveness as an inducer of mitotic catastrophe. Curcumin, but not vincristine, acts on HCW-2 cells by inhibiting the expression of survivin, a modulator of cell division and apoptosis in cancer. Altogether our results show that apoptosis resistance can be overcome by inducing mitotic catastrophe in HCW-2 cells. © 2006 Wiley-Liss, Inc.
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