New therapeutic targets in ulcerative colitis: The importance of ion transporters in the human colon
Klaudia Farkas MD
First Department of Medicine, University of Szeged, Szeged, Hungary
The first two authors contributed equally.
Search for more papers by this authorSunil Yeruva MSc, PhD
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
The first two authors contributed equally.
Search for more papers by this authorZoltán Rakonczay Jr MD, PhD
First Department of Medicine, University of Szeged, Szeged, Hungary
Search for more papers by this authorLisa Ludolph MSc
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
Search for more papers by this authorTamás Molnár MD, PhD
First Department of Medicine, University of Szeged, Szeged, Hungary
Search for more papers by this authorFerenc Nagy MD, PhD
First Department of Medicine, University of Szeged, Szeged, Hungary
Search for more papers by this authorZoltán Szepes MD, PhD
First Department of Medicine, University of Szeged, Szeged, Hungary
Search for more papers by this authorAndrea Schnúr MSc
First Department of Medicine, University of Szeged, Szeged, Hungary
Search for more papers by this authorTibor Wittmann MD, PhD
First Department of Medicine, University of Szeged, Szeged, Hungary
Search for more papers by this authorJessica Hubricht MD
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
Search for more papers by this authorBrigitte Riederer MSc, PhD
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
Search for more papers by this authorViktória Venglovecz MSc, PhD
Department of Pharmacology and Pharmacotherapy, University of Szeged, Szeged, Hungary
Search for more papers by this authorGyörgy Lázár MD, PhD
Department of Surgery, University of Szeged, Szeged, Hungary
Search for more papers by this authorMarianna Király MSc
Department of Oral Biology, Semmelweis University, Budapest, Hungary
Search for more papers by this authorÁkos Zsembery MD, PhD
Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary
Search for more papers by this authorGábor Varga MSc, PhD, DSc
Department of Oral Biology, Semmelweis University, Budapest, Hungary
Search for more papers by this authorUrsula Seidler XX
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
Search for more papers by this authorCorresponding Author
Péter Hegyi MD, PhD
First Department of Medicine, University of Szeged, Szeged, Hungary
First Department of Medicine, University of Szeged, H-6720, Korányi fasor 8-10, Szeged, HungarySearch for more papers by this authorKlaudia Farkas MD
First Department of Medicine, University of Szeged, Szeged, Hungary
The first two authors contributed equally.
Search for more papers by this authorSunil Yeruva MSc, PhD
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
The first two authors contributed equally.
Search for more papers by this authorZoltán Rakonczay Jr MD, PhD
First Department of Medicine, University of Szeged, Szeged, Hungary
Search for more papers by this authorLisa Ludolph MSc
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
Search for more papers by this authorTamás Molnár MD, PhD
First Department of Medicine, University of Szeged, Szeged, Hungary
Search for more papers by this authorFerenc Nagy MD, PhD
First Department of Medicine, University of Szeged, Szeged, Hungary
Search for more papers by this authorZoltán Szepes MD, PhD
First Department of Medicine, University of Szeged, Szeged, Hungary
Search for more papers by this authorAndrea Schnúr MSc
First Department of Medicine, University of Szeged, Szeged, Hungary
Search for more papers by this authorTibor Wittmann MD, PhD
First Department of Medicine, University of Szeged, Szeged, Hungary
Search for more papers by this authorJessica Hubricht MD
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
Search for more papers by this authorBrigitte Riederer MSc, PhD
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
Search for more papers by this authorViktória Venglovecz MSc, PhD
Department of Pharmacology and Pharmacotherapy, University of Szeged, Szeged, Hungary
Search for more papers by this authorGyörgy Lázár MD, PhD
Department of Surgery, University of Szeged, Szeged, Hungary
Search for more papers by this authorMarianna Király MSc
Department of Oral Biology, Semmelweis University, Budapest, Hungary
Search for more papers by this authorÁkos Zsembery MD, PhD
Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary
Search for more papers by this authorGábor Varga MSc, PhD, DSc
Department of Oral Biology, Semmelweis University, Budapest, Hungary
Search for more papers by this authorUrsula Seidler XX
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
Search for more papers by this authorCorresponding Author
Péter Hegyi MD, PhD
First Department of Medicine, University of Szeged, Szeged, Hungary
First Department of Medicine, University of Szeged, H-6720, Korányi fasor 8-10, Szeged, HungarySearch for more papers by this authorAbstract
Background:
The absorption of water and ions (especially Na+ and Cl−) is an important function of colonic epithelial cells in both physiological and pathophysiological conditions. Despite the comprehensive animal studies, there are only scarce available data on the ion transporter activities of the normal and inflamed human colon.
Methods:
In this study, 128 healthy controls and 69 patients suffering from ulcerative colitis (UC) were involved. We investigated the expressional and functional characteristics of the Na+/H+ exchangers (NHE) 1–3, the epithelial sodium channel (ENaC), and the SLC26A3 Cl−/HCO
exchanger downregulated in adenoma (DRA) in primary colonic crypts isolated from human biopsy and surgical samples using microfluorometry, patch clamp, and real-time reverse-transcription polymerase chain reaction (RT-PCR) techniques.
Results:
Data collected from colonic crypts showed that the activities of electroneutral (via NHE3) and the electrogenic Na+ absorption (via ENaC) are in inverse ratio to each other in the proximal and distal colon. We found no significant differences in the activity of NHE2 in different segments of the colon. Surface cell Cl−/HCO
exchange is more active in the distal part of the colon. Importantly, both sodium and chloride absorptions are damaged in UC, whereas NHE1, which has been shown to promote immune response, is upregulated by 6-fold.
Conclusions:
These results open up new therapeutic targets in UC. (Inflamm Bowel Dis 2011;)
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