Overexpression of HMGA2 in uterine leiomyomas points to its general role for the pathogenesis of the disease†
Markus Klemke
Center for Human Genetics, University of Bremen, 28359 Bremen, Germany
Markus Klemke and Anke Meyer contributed equally to this work.
Search for more papers by this authorAnke Meyer
Center for Human Genetics, University of Bremen, 28359 Bremen, Germany
Markus Klemke and Anke Meyer contributed equally to this work.
Search for more papers by this authorMaliheh Hashemi Nezhad
Center for Human Genetics, University of Bremen, 28359 Bremen, Germany
Search for more papers by this authorSabine Bartnitzke
Center for Human Genetics, University of Bremen, 28359 Bremen, Germany
Search for more papers by this authorNorbert Drieschner
Center for Human Genetics, University of Bremen, 28359 Bremen, Germany
Search for more papers by this authorChristiane Frantzen
Women's Clinic, St. Joseph-Stift Hospital, 28209 Bremen, Germany
Search for more papers by this authorErnst Heinrich Schmidt
Department of Obstetrics and Gynecology, DIAKO Evang. Diakonie Hospital, 28239 Bremen, Germany
Search for more papers by this authorGazanfer Belge
Center for Human Genetics, University of Bremen, 28359 Bremen, Germany
Search for more papers by this authorCorresponding Author
Jörn Bullerdiek
Center for Human Genetics, University of Bremen, 28359 Bremen, Germany
Clinic for Small Animals and Research Cluster REBIRTH, University of Veterinary Medicine, 30137 Hanover, Germany
Center for Human Genetics, University of Bremen, Leobener Str. ZHG, 28359 Bremen, GermanySearch for more papers by this authorMarkus Klemke
Center for Human Genetics, University of Bremen, 28359 Bremen, Germany
Markus Klemke and Anke Meyer contributed equally to this work.
Search for more papers by this authorAnke Meyer
Center for Human Genetics, University of Bremen, 28359 Bremen, Germany
Markus Klemke and Anke Meyer contributed equally to this work.
Search for more papers by this authorMaliheh Hashemi Nezhad
Center for Human Genetics, University of Bremen, 28359 Bremen, Germany
Search for more papers by this authorSabine Bartnitzke
Center for Human Genetics, University of Bremen, 28359 Bremen, Germany
Search for more papers by this authorNorbert Drieschner
Center for Human Genetics, University of Bremen, 28359 Bremen, Germany
Search for more papers by this authorChristiane Frantzen
Women's Clinic, St. Joseph-Stift Hospital, 28209 Bremen, Germany
Search for more papers by this authorErnst Heinrich Schmidt
Department of Obstetrics and Gynecology, DIAKO Evang. Diakonie Hospital, 28239 Bremen, Germany
Search for more papers by this authorGazanfer Belge
Center for Human Genetics, University of Bremen, 28359 Bremen, Germany
Search for more papers by this authorCorresponding Author
Jörn Bullerdiek
Center for Human Genetics, University of Bremen, 28359 Bremen, Germany
Clinic for Small Animals and Research Cluster REBIRTH, University of Veterinary Medicine, 30137 Hanover, Germany
Center for Human Genetics, University of Bremen, Leobener Str. ZHG, 28359 Bremen, GermanySearch for more papers by this authorSupported by: Tönjes-Vagt-Stiftung, Bremen.
Abstract
An overexpression of HMGA2 is supposed to be a key event in the genesis of leiomyoma with chromosomal rearrangements affecting the region 12q14-15 targeting the HMGA2 gene, but gene expression data regarding differences between uterine leiomyomas with and those without 12q14-15 aberrations are insufficient. To address the question whether HMGA2 is only upregulated in the 12q14-15 subgroup, the expression of HMGA2 was analyzed in a comprehensive set of leiomyomas (n = 180) including tumors with 12q14-15 chromosomal aberrations (n = 13) and matching myometrial tissues (n = 51) by quantitative RT-PCR. The highest expression levels for HMGA2 were observed in tumors with rearrangements affecting the region 12q14-15, but although HMGA2 is expressed at lower levels in leiomyomas without such aberrations, the comparison between the expression in myomas and matching myometrial tissues indicates a general upregulation of HMGA2 regardless of the presence or absence of such chromosomal abnormalities. The significant (P < 0.05) overexpression of HMGA2 also in the group of fibroids without chromosomal aberrations of the 12q14-15 region suggests a general role of HMGA2 in the development of the disease. © 2008 Wiley-Liss, Inc.
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