Enzymatic Synthesis of a Diastereomer of Neoabyssomicin Derivative Using the Diels-Alderase AbyU
Wenjuan Ding
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, Guangdong, 510301 China
College of Oceanology, University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049 China
Search for more papers by this authorChangbiao Chi
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191 China
Search for more papers by this authorXiaoyi Wei
Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, 510650 China
Search for more papers by this authorChangli Sun
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, Guangdong, 510301 China
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), No. 1119, Haibin Rd., Nansha District, Guangzhou, Guangdong, 510301 China
Search for more papers by this authorJiajia Tu
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, Guangdong, 510301 China
Search for more papers by this authorMing Ma
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191 China
Search for more papers by this authorCorresponding Author
Qinglian Li
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, Guangdong, 510301 China
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), No. 1119, Haibin Rd., Nansha District, Guangzhou, Guangdong, 510301 China
E-mail: [email protected]; [email protected]Search for more papers by this authorCorresponding Author
Jianhua Ju
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, Guangdong, 510301 China
College of Oceanology, University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049 China
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), No. 1119, Haibin Rd., Nansha District, Guangzhou, Guangdong, 510301 China
E-mail: [email protected]; [email protected]Search for more papers by this authorWenjuan Ding
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, Guangdong, 510301 China
College of Oceanology, University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049 China
Search for more papers by this authorChangbiao Chi
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191 China
Search for more papers by this authorXiaoyi Wei
Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, 510650 China
Search for more papers by this authorChangli Sun
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, Guangdong, 510301 China
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), No. 1119, Haibin Rd., Nansha District, Guangzhou, Guangdong, 510301 China
Search for more papers by this authorJiajia Tu
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, Guangdong, 510301 China
Search for more papers by this authorMing Ma
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191 China
Search for more papers by this authorCorresponding Author
Qinglian Li
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, Guangdong, 510301 China
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), No. 1119, Haibin Rd., Nansha District, Guangzhou, Guangdong, 510301 China
E-mail: [email protected]; [email protected]Search for more papers by this authorCorresponding Author
Jianhua Ju
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, Guangdong, 510301 China
College of Oceanology, University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049 China
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), No. 1119, Haibin Rd., Nansha District, Guangzhou, Guangdong, 510301 China
E-mail: [email protected]; [email protected]Search for more papers by this authorMain observation and conclusion
The enzyme AbyU catalyses a Diels-Alder (DA) reaction during abyssomicin C biosynthesis. In this study, AbyU is shown to convert the native substrate of another Diels-Alderase (DAase), AbmU, to a new abyssomicin derivative, abyssomicin 7. Abyssomicin 7 is a diastereomer of the AbmU-derived, abyssomicin 6. Using structural analyses and site-directed mutagenesis, we unveil the molecular basis for production of abyssomicin 7, instead of abyssomicin 6, within the AbyU active site.
Supporting Information
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Appendix S1: Supporting Information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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