Review
Advances and Challenges in Fluorescence-Based Biosensors for Biological and Chemical Detection
Salha Alharthi,
Sarah Alharthi,
Mohamed Madani,
Huda Alkhaldi,
Nadiah Aldaleeli,
Albandari Almarri,
Fatmah Alnass,
Dalal Alzahrani,
Safwat A. Mahmoud,
Md Azizul Haque,
Mohamed S. Attia,
Ahmed Siddiq,
S. S. Aly,
Mohamed Mohamady Ghobashy,
Salha Alharthi
Chemistry Department, College of Science, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, 31441 Saudi Arabia
Search for more papers by this authorSarah Alharthi
Department of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif, 21944 Saudi Arabia
Search for more papers by this authorMohamed Madani
Department of Physics, College of Science and Humanities-Jubail, Imam Abdulrahman Bin Faisal University, Jubail, 35811 Saudi Arabia
Search for more papers by this authorHuda Alkhaldi
Department of Physics, College of Science and Humanities-Jubail, Imam Abdulrahman Bin Faisal University, Jubail, 35811 Saudi Arabia
Search for more papers by this authorNadiah Aldaleeli
Department of Physics, College of Science and Humanities-Jubail, Imam Abdulrahman Bin Faisal University, Jubail, 35811 Saudi Arabia
Search for more papers by this authorAlbandari Almarri
Department of Physics, College of Science and Humanities-Jubail, Imam Abdulrahman Bin Faisal University, Jubail, 35811 Saudi Arabia
Search for more papers by this authorFatmah Alnass
Department of Physics, College of Science and Humanities-Jubail, Imam Abdulrahman Bin Faisal University, Jubail, 35811 Saudi Arabia
Search for more papers by this authorDalal Alzahrani
College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Jubail, 31441 Saudi Arabia
Search for more papers by this authorSafwat A. Mahmoud
Center for Scientific Research and Entrepreneurship, Northern Border University, Arar, 73213 Saudi Arabia
Search for more papers by this authorCorresponding Author
Md Azizul Haque
Department of Biotechnology, Yeungnam University, Gyeongbuk, 38541 Republic of Korea
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Mohamed S. Attia
Chemistry Department, Faculty of Science, Ain Shams University, Abbassia, Cairo, 11566 Egypt
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorAhmed Siddiq
Department of Chemistry, Faculty of Science, Al-Azhar University, Assiut, 71524 Egypt
Search for more papers by this authorS. S. Aly
Department of Physics, College of Science, Qassim University, Buraydah, 51452 Saudi Arabia
Search for more papers by this authorCorresponding Author
Mohamed Mohamady Ghobashy
Radiation Research of Polymer Chemistry Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorSalha Alharthi,
Sarah Alharthi,
Mohamed Madani,
Huda Alkhaldi,
Nadiah Aldaleeli,
Albandari Almarri,
Fatmah Alnass,
Dalal Alzahrani,
Safwat A. Mahmoud,
Md Azizul Haque,
Mohamed S. Attia,
Ahmed Siddiq,
S. S. Aly,
Mohamed Mohamady Ghobashy,
Salha Alharthi
Chemistry Department, College of Science, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, 31441 Saudi Arabia
Search for more papers by this authorSarah Alharthi
Department of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif, 21944 Saudi Arabia
Search for more papers by this authorMohamed Madani
Department of Physics, College of Science and Humanities-Jubail, Imam Abdulrahman Bin Faisal University, Jubail, 35811 Saudi Arabia
Search for more papers by this authorHuda Alkhaldi
Department of Physics, College of Science and Humanities-Jubail, Imam Abdulrahman Bin Faisal University, Jubail, 35811 Saudi Arabia
Search for more papers by this authorNadiah Aldaleeli
Department of Physics, College of Science and Humanities-Jubail, Imam Abdulrahman Bin Faisal University, Jubail, 35811 Saudi Arabia
Search for more papers by this authorAlbandari Almarri
Department of Physics, College of Science and Humanities-Jubail, Imam Abdulrahman Bin Faisal University, Jubail, 35811 Saudi Arabia
Search for more papers by this authorFatmah Alnass
Department of Physics, College of Science and Humanities-Jubail, Imam Abdulrahman Bin Faisal University, Jubail, 35811 Saudi Arabia
Search for more papers by this authorDalal Alzahrani
College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Jubail, 31441 Saudi Arabia
Search for more papers by this authorSafwat A. Mahmoud
Center for Scientific Research and Entrepreneurship, Northern Border University, Arar, 73213 Saudi Arabia
Search for more papers by this authorCorresponding Author
Md Azizul Haque
Department of Biotechnology, Yeungnam University, Gyeongbuk, 38541 Republic of Korea
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Mohamed S. Attia
Chemistry Department, Faculty of Science, Ain Shams University, Abbassia, Cairo, 11566 Egypt
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorAhmed Siddiq
Department of Chemistry, Faculty of Science, Al-Azhar University, Assiut, 71524 Egypt
Search for more papers by this authorS. S. Aly
Department of Physics, College of Science, Qassim University, Buraydah, 51452 Saudi Arabia
Search for more papers by this authorCorresponding Author
Mohamed Mohamady Ghobashy
Radiation Research of Polymer Chemistry Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorFirst published: 19 April 2025
Abstract
Fluorescence-based biosensors have become highly effective analytical instruments, revolutionizing the landscape of biological and chemical detection across diverse fields. These devices harness the unique properties of fluorescence to detect and quantify a wide array of analytes with remarkable sensitivity and specificity. By integrating biological recognition elements with advanced optical technologies, fluorescence-based biosensors provide high sensitivity, rapid detection, and real-time monitoring capabilities for diagnostic applications. This review discusses the fundamental principles of fluorescence biosensing, its key advantages, and the various types. The applications of these sensors span fields such as medicine, monitoring of the environment, food safety, biosecurity, and agriculture. Although fluorescence biosensors offer numerous benefits, the review also addresses the ongoing challenges and limitations researchers are working to overcome, such as photobleaching, background autofluorescence, and limited tissue penetration depth. The review emphasizes the critical design considerations and the continuous advancements in developing these innovative analytical tools, which are poised to transform the future of biological and chemical detection.
Conflicts of Interest
The authors declare that they have no conflicts of interest.
Open Research
Data Availability Statement
All data generated or analyzed during this study are included in this manuscript and available.
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