Archives of Insect Biochemistry and Physiology

Volume 96, Issue 4 e21429

ARTICLE

Silencing pyruvate kinase (NlPYK) leads to reduced fecundity in brown planthoppers, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

Lin-Quan Ge,

Corresponding Author

Lin-Quan Ge

[email protected]

orcid.org/0000-0002-9590-1295

School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, P. R. China

These authors are co-first authors.

Correspondence

LinQuan Ge, School of Plant Protection, Yangzhou University, Wenhui East Road 48, Yangzhou, Jiangsu Province 225009, P. R. China.

Email: [email protected]

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Bo Huang,

Bo Huang

School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, P. R. China

These authors are co-first authors.

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Xin Li,

Xin Li

School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, P. R. China

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Hao-Tian Gu,

Hao-Tian Gu

School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, P. R. China

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Sui Zheng,

Sui Zheng

School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, P. R. China

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Ze Zhou,

Ze Zhou

School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, P. R. China

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Hong Miao,

Hong Miao

College of Mechanical Engineering, Yangzhou University, Yangzhou, P. R. China

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Jin-Cai Wu,

Jin-Cai Wu

School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, P. R. China

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Lin-Quan Ge,

Corresponding Author

Lin-Quan Ge

[email protected]

orcid.org/0000-0002-9590-1295

School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, P. R. China

These authors are co-first authors.

Correspondence

LinQuan Ge, School of Plant Protection, Yangzhou University, Wenhui East Road 48, Yangzhou, Jiangsu Province 225009, P. R. China.

Email: [email protected]

Search for more papers by this author

Bo Huang,

Bo Huang

School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, P. R. China

These authors are co-first authors.

Search for more papers by this author

Xin Li,

Xin Li

School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, P. R. China

Search for more papers by this author

Hao-Tian Gu,

Hao-Tian Gu

School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, P. R. China

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Sui Zheng,

Sui Zheng

School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, P. R. China

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Ze Zhou,

Ze Zhou

School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, P. R. China

Search for more papers by this author

Hong Miao,

Hong Miao

College of Mechanical Engineering, Yangzhou University, Yangzhou, P. R. China

Search for more papers by this author

Jin-Cai Wu,

Jin-Cai Wu

School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, P. R. China

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First published: 07 November 2017

https://doi.org/10.1002/arch.21429

Citations: 12

Funding information

Grant sponsor: National Key R & D Program of China; Grant number: 2017YFD0200400; Grant sponsor: Natural Science Foundation of Jiangsu Province, China; Grant number: BK20171283; Grant sponsor: National Natural Science Foundation of China; Grant number: 31371938; Grant sponsor: Innovative Cultivation Foundation of Yangzhou University; Grant number: 2016CXJ065; Grant sponsor: the Jiangsu Agricultural Scientific Self-innovation Foundation; Grant number: (CX(15)1057); Grant sponsor: the Foundation of Science and Technology of Jiangsu Province, People's Republic of China; Grant number: BE2015342.

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Abstract

Pyruvate kinase (PYK) operates in the glycolytic pathway, responsible for regulating the balance between glycolysis and gluconeogenesis. The previous work indicates PYK acts in development of Drosophila embryos and in embryonic muscle growth, from which it may be inferred that PYK acts in insect fecundity. More to the point, as a central enzyme in the glycolytic pathway, PYK acts in many energy-spending functions in most organisms. On the background findings that triazophos (TZP) stimulates fecundity via increase activities of several genes in brown planthoppers, Nilaparvata lugens, we investigated the combined influence of TZP and silencing a N. lugens PYK (NlPYK) on reproduction-linked biological performance parameters. Here, we report that TZP+dsNlPYK treatments led to reduced (by 26%) ovarian, but not fat body, protein content relative to controls. Ovarian (35%) and fat body (54%) soluble sugar contents were reduced. TZP+dsNlPYK treatments also led to reduced (by about 24%) fecundity, expressed as numbers of eggs laid. These data show directly that NlPYK acts in insect fecundity, probably via increases in glucose metabolism.

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Volume96, Issue4

December 2017

e21429

Silencing pyruvate kinase (NlPYK) leads to reduced fecundity in brown planthoppers, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

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Silencing pyruvate kinase (NlPYK) leads to reduced fecundity in brown planthoppers, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

Abstract

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Citing Literature

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