Peptide-Grafted Waterborne Polyurethane With Enhanced Biocompatibility and Mechanical Properties for Biomedical Applications
Yun Hong
CAS Key Laboratory of High-Performance Synthetic Rubber and its Composite Materials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, China
Contribution: Conceptualization (lead), Formal analysis (lead), Investigation (lead), Methodology (lead), Writing - original draft (lead), Writing - review & editing (lead)
Search for more papers by this authorChunjie Li
CAS Key Laboratory of High-Performance Synthetic Rubber and its Composite Materials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
Contribution: Formal analysis (equal), Investigation (equal), Methodology (equal)
Search for more papers by this authorFeng Zhang
CAS Key Laboratory of High-Performance Synthetic Rubber and its Composite Materials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
Contribution: Formal analysis (equal), Investigation (equal), Methodology (equal)
Search for more papers by this authorCorresponding Author
Xiaoye Ma
CAS Key Laboratory of High-Performance Synthetic Rubber and its Composite Materials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
Correspondence:
Chuanqing Kang ([email protected])
Xiaoye Ma ([email protected])
Contribution: Data curation (lead), Project administration (lead), Writing - review & editing (lead)
Search for more papers by this authorDingxiao Jiang
CAS Key Laboratory of High-Performance Synthetic Rubber and its Composite Materials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, China
Contribution: Investigation (supporting), Methodology (supporting)
Search for more papers by this authorRizhe Jin
CAS Key Laboratory of High-Performance Synthetic Rubber and its Composite Materials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
Contribution: Methodology (equal)
Search for more papers by this authorCorresponding Author
Chuanqing Kang
CAS Key Laboratory of High-Performance Synthetic Rubber and its Composite Materials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, China
Correspondence:
Chuanqing Kang ([email protected])
Xiaoye Ma ([email protected])
Contribution: Conceptualization (lead), Funding acquisition (lead), Supervision (lead), Writing - review & editing (lead)
Search for more papers by this authorYun Hong
CAS Key Laboratory of High-Performance Synthetic Rubber and its Composite Materials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, China
Contribution: Conceptualization (lead), Formal analysis (lead), Investigation (lead), Methodology (lead), Writing - original draft (lead), Writing - review & editing (lead)
Search for more papers by this authorChunjie Li
CAS Key Laboratory of High-Performance Synthetic Rubber and its Composite Materials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
Contribution: Formal analysis (equal), Investigation (equal), Methodology (equal)
Search for more papers by this authorFeng Zhang
CAS Key Laboratory of High-Performance Synthetic Rubber and its Composite Materials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
Contribution: Formal analysis (equal), Investigation (equal), Methodology (equal)
Search for more papers by this authorCorresponding Author
Xiaoye Ma
CAS Key Laboratory of High-Performance Synthetic Rubber and its Composite Materials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
Correspondence:
Chuanqing Kang ([email protected])
Xiaoye Ma ([email protected])
Contribution: Data curation (lead), Project administration (lead), Writing - review & editing (lead)
Search for more papers by this authorDingxiao Jiang
CAS Key Laboratory of High-Performance Synthetic Rubber and its Composite Materials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, China
Contribution: Investigation (supporting), Methodology (supporting)
Search for more papers by this authorRizhe Jin
CAS Key Laboratory of High-Performance Synthetic Rubber and its Composite Materials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
Contribution: Methodology (equal)
Search for more papers by this authorCorresponding Author
Chuanqing Kang
CAS Key Laboratory of High-Performance Synthetic Rubber and its Composite Materials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, China
Correspondence:
Chuanqing Kang ([email protected])
Xiaoye Ma ([email protected])
Contribution: Conceptualization (lead), Funding acquisition (lead), Supervision (lead), Writing - review & editing (lead)
Search for more papers by this authorFunding: This work was supported by the Science and Technology Project of Jilin Province, China (No. SKL202302036).
ABSTRACT
The incorporation of fillers into waterborne polyurethane (WPU) can endow the composites with improved physicochemical and physiological properties for biomedical applications. Silk fibroin possesses excellent mechanical properties and biocompatibility owing to its unique hierarchical structure comprising β-sheet crystallites and amorphous matrix. To mimic its structure, a crystalline tetrapeptide Gly-Ala-Gly-Ala (GAGA) derived from the repetitive segments of the primary sequence of fibroins was grafted onto the WPU backbone. The tetrapeptides aggregated via additional hydrogen bonds to form crystalline domains, which served as physical crosslinking points to significantly improve the mechanical properties of WPU. Compared with the pristine WPU, the modification enhanced the maximum tensile strength (from 13.1 to 25.4 MPa) and hydrophilicity (the water contact angle from 80.6° to 38.8°). In addition, the modified WPU with the optimal proportion of the tetrapeptide remained structurally stable under physiological environments and showed improved biocompatibility including good cell and protein adhesion, noncytotoxicity, and nonhemolysis. The excellent performance confirms the effectiveness of peptides in WPU modification and sheds light on the potential of modified WPU in biomedical applications.
Conflicts of Interest
The authors declare no conflicts of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
| Filename | Description |
|---|---|
| app56695-sup-0001-supinfo.docxWord 2007 document , 2.5 MB |
Data S1. Supporting Information. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
References
- 1 E. Bruno, G. Borea, R. Valeriani, et al., “Evaluating the Quality of Online Patient Information for Prepectoral Breast Reconstruction Using Polyurethane-Coated Breast Implants,” JPRAS Open 39 (2023): 11–17, https://doi.org/10.1016/j.jpra.2023.10.015.
- 2 Z. Miri, S. Farè, Q. Ma, and H. J. Haugen, “Updates on Polyurethane and Its Multifunctional Applications in Biomedical Engineering,” Progress in Biomedical Engineering 5, no. 4 (2023): 042001, https://doi.org/10.1088/2516-1091/acef84.
- 3 S. Li, H. Zhang, J. Xie, et al., “In Vivo Self-Assembled Shape-Memory Polyurethane for Minimally Invasive Delivery and Therapy,” Materials Horizons 10, no. 9 (2023): 3438–3449, https://doi.org/10.1039/d3mh00594a.
- 4 T. Selvaras, S. A. Alshamrani, R. Gopal, et al., “Biodegradable and Antithrombogenic Chitosan/Elastin Blended Polyurethane Electrospun Membrane for Vascular Tissue Integration,” Journal of Biomedical Materials Research. Part B, Applied Biomaterials 111, no. 6 (2023): 1171–1181, https://doi.org/10.1002/jbm.b.35223.
- 5 A. Robinson, A. Nkansah, S. Bhat, et al., “Hydrogel-Polyurethane Fiber Composites With Enhanced Microarchitectural Control for Heart Valve Replacement,” Journal of Biomedical Materials Research: Part A 112 (2023): 586–599, https://doi.org/10.1002/jbm.a.37641.
- 6 F. Guo, R. Han, J. Ying, Z. Zhang, R. Yang, and X. Zhang, “Bioinspired Polymeric Heart Valves Derived From Polyurethane and Natural Cellulose Fibers,” Journal of Materials Science and Technology 144 (2023): 178–187, https://doi.org/10.1016/j.eurpolymj.2019.109294.
- 7 M. Crago, A. Lee, S. Farajikhah, et al., “The Evolution of Polyurethane Heart Valve Replacements: How Chemistry Translates to the Clinic,” Materials Today Communications 33 (2022): 104916, https://doi.org/10.1016/j.mtcomm.2022.104916.
- 8 A. Das, A. Nikhil, P. A. Shiekh, B. Yadav, K. Jagavelu, and A. Kumar, “Ameliorating Impaired Cardiac Function in Myocardial Infarction Using Exosome-Loaded Gallic-Acid-Containing Polyurethane Scaffolds,” Bioactive Materials 33 (2023): 324–340, https://doi.org/10.1016/j.bioactmat.2023.11.009.
- 9 J. Guan, K. L. Fujimoto, and W. R. Wagner, “Elastase-Sensitive Elastomeric Scaffolds With Variable Anisotropy for Soft Tissue Engineering,” Pharmaceutical Research 25, no. 10 (2008): 2400–2412, https://doi.org/10.1007/s11095-008-9628-x.
- 10 V. García-Pacios, V. Costa, M. Colera, and J. M. Martín-Martínez, “Waterborne Polyurethane Dispersions Obtained With Polycarbonate of Hexanediol Intended for Use as Coatings,” Progress in Organic Coating 71, no. 2 (2011): 136–146, https://doi.org/10.1016/j.porgcoat.2011.01.006.
- 11 X. Yin, L. Li, H. Pang, Y. Luo, and B. Zhang, “Halogen-Free Instinct Flame-Retardant Waterborne Polyurethanes: Composition, Performance, and Application,” RSC Advances 12, no. 23 (2022): 14509–14520, https://doi.org/10.1039/d2ra01822e.
- 12 X. Cao, P. R. Chang, and M. A. Huneault, “Preparation and Properties of Plasticized Starch Modified With Poly (ε-Caprolactone) Based Waterborne Polyurethane,” Carbohydrate Polymers 71, no. 1 (2008): 119–125, https://doi.org/10.1016/j.carbpol.2007.05.023.
- 13 Y. Wang, H. Tian, and L. Zhang, “Role of Starch Nanocrystals and Cellulose Whiskers in Synergistic Reinforcement of Waterborne Polyurethane,” Carbohydrate Polymers 80, no. 3 (2010): 665–671, https://doi.org/10.1016/j.carbpol.2009.10.043.
- 14 Y. Lu, L. Tighzert, F. Berzin, and S. Rondot, “Innovative Plasticized Starch Films Modified With Waterborne Polyurethane From Renewable Resources,” Carbohydrate Polymers 61, no. 2 (2005): 174–182, https://doi.org/10.1016/j.carbpol.2005.04.013.
- 15 Y. Fan, X. Song, L. Liu, C. Zhou, and G. Wu, “Dual Network Interpenetrating Degradable Waterborne Polyurethanes With Hydrogen Bonded Cross-Linked Modified β-Cyclodextrin Hydrophobic Cavities,” Journal of Applied Polymer Science 140, no. 33 (2023): e54298, https://doi.org/10.1002/app.54298.
- 16 X. Lu, L. Cao, X. Yin, Y. Si, J. Yu, and B. Ding, “Stretchable, Tough and Elastic Nanofibrous Hydrogels With Dermis-Mimicking Network Structure,” Journal of Colloid and Interface Science 582 (2021): 387–395, https://doi.org/10.1016/j.jcis.2020.08.020.
- 17 Y. Tao, A. Hasan, G. Deeb, C. Hu, and H. Han, “Rheological and Mechanical Behavior of Silk Fibroin Reinforced Waterborne Polyurethane,” Polymers 8, no. 3 (2016): 94, https://doi.org/10.3390/polym8030094.
- 18 L. D. Koh, Y. Cheng, C. P. Teng, et al., “Structures, Mechanical Properties and Applications of Silk Fibroin Materials,” Progress in Polymer Science 46 (2015): 86–110, https://doi.org/10.1016/j.progpolymsci.2015.02.001.
- 19 S. Fan, Y. Zhang, X. Huang, et al., “Silk Materials for Medical, Electronic and Optical Applications,” Science China Technological Sciences 62 (2019): 903–918, https://doi.org/10.1007/s11431-018-9403-8.
- 20 Y. Suzuki, “Structures of Silk Fibroin Before and After Spinning and Biomedical Applications,” Polymer Journal 48, no. 11 (2016): 1039–1044, https://doi.org/10.1038/pj.2016.77.
- 21 F. Costa, R. Silva, and A. R. Boccaccini, Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair (Cambridge, UK: Woodhead Publishing, 2018).
- 22 R. V. Lewis, “Spider Silk: Ancient Ideas for New Biomaterials,” Chemical Reviews 106, no. 9 (2006): 3762–3774, https://doi.org/10.1021/cr010194g.
- 23 Y. Huang, B. Zhang, G. Xu, and W. Hao, “Swelling Behaviors and Mechanical Properties of Silk Fibroin–Polyurethane Composite Hydrogels,” Composites Science and Technology 84 (2013): 15–22, https://doi.org/10.1016/j.compscitech.2013.05.007.
- 24 H. S. Park, M. S. Gong, J. H. Park, et al., “Silk Fibroin-Polyurethane Blends: Physical Properties and Effect of Silk Fibroin Content on Viscoelasticity, Biocompatibility and Myoblast Differentiation,” Acta Biomaterialia 9, no. 11 (2013): 8962–8971, https://doi.org/10.1016/j.actbio.2013.07.013.
