A novel icariin-encapsulated PLGA/nBM composite biomimetic microspheres for bone repair
Zhenxu Wu
Department of Orthopedics, Foshan Hospital of Traditional Chinese Medicine/Foshan Hospital of TCM, Foshan, China
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
Contribution: Conceptualization (equal), Data curation (lead), Formal analysis (lead), Investigation (lead), Writing - original draft (lead), Writing - review & editing (lead)
Search for more papers by this authorGuoqing Niu
Department of Orthopedics, Foshan Hospital of Traditional Chinese Medicine/Foshan Hospital of TCM, Foshan, China
Contribution: Investigation (supporting)
Search for more papers by this authorZongtai Xiong
Department of Orthopedics, Foshan Hospital of Traditional Chinese Medicine/Foshan Hospital of TCM, Foshan, China
Contribution: Investigation (supporting)
Search for more papers by this authorXinxiang Ding
Department of Orthopedics, Foshan Hospital of Traditional Chinese Medicine/Foshan Hospital of TCM, Foshan, China
Contribution: Investigation (supporting)
Search for more papers by this authorYongbo Li
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, China
Contribution: Resources (supporting)
Search for more papers by this authorCorresponding Author
Peibiao Zhang
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
Correspondence
Feng Wu, Department of Orthopedics, Foshan Hospital of Traditional Chinese Medicine/Foshan Hospital of TCM, Foshan, China.
Email: [email protected]
Peibiao Zhang, Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
Email: [email protected]
Contribution: Conceptualization (lead), Funding acquisition (lead), Project administration (lead), Writing - review & editing (lead)
Search for more papers by this authorCorresponding Author
Feng Wu
Department of Orthopedics, Foshan Hospital of Traditional Chinese Medicine/Foshan Hospital of TCM, Foshan, China
Correspondence
Feng Wu, Department of Orthopedics, Foshan Hospital of Traditional Chinese Medicine/Foshan Hospital of TCM, Foshan, China.
Email: [email protected]
Peibiao Zhang, Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
Email: [email protected]
Contribution: Conceptualization (lead), Funding acquisition (lead), Project administration (lead), Resources (lead)
Search for more papers by this authorZhenxu Wu
Department of Orthopedics, Foshan Hospital of Traditional Chinese Medicine/Foshan Hospital of TCM, Foshan, China
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
Contribution: Conceptualization (equal), Data curation (lead), Formal analysis (lead), Investigation (lead), Writing - original draft (lead), Writing - review & editing (lead)
Search for more papers by this authorGuoqing Niu
Department of Orthopedics, Foshan Hospital of Traditional Chinese Medicine/Foshan Hospital of TCM, Foshan, China
Contribution: Investigation (supporting)
Search for more papers by this authorZongtai Xiong
Department of Orthopedics, Foshan Hospital of Traditional Chinese Medicine/Foshan Hospital of TCM, Foshan, China
Contribution: Investigation (supporting)
Search for more papers by this authorXinxiang Ding
Department of Orthopedics, Foshan Hospital of Traditional Chinese Medicine/Foshan Hospital of TCM, Foshan, China
Contribution: Investigation (supporting)
Search for more papers by this authorYongbo Li
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, China
Contribution: Resources (supporting)
Search for more papers by this authorCorresponding Author
Peibiao Zhang
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
Correspondence
Feng Wu, Department of Orthopedics, Foshan Hospital of Traditional Chinese Medicine/Foshan Hospital of TCM, Foshan, China.
Email: [email protected]
Peibiao Zhang, Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
Email: [email protected]
Contribution: Conceptualization (lead), Funding acquisition (lead), Project administration (lead), Writing - review & editing (lead)
Search for more papers by this authorCorresponding Author
Feng Wu
Department of Orthopedics, Foshan Hospital of Traditional Chinese Medicine/Foshan Hospital of TCM, Foshan, China
Correspondence
Feng Wu, Department of Orthopedics, Foshan Hospital of Traditional Chinese Medicine/Foshan Hospital of TCM, Foshan, China.
Email: [email protected]
Peibiao Zhang, Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
Email: [email protected]
Contribution: Conceptualization (lead), Funding acquisition (lead), Project administration (lead), Resources (lead)
Search for more papers by this authorAbstract
Deer bone in traditional Chinese medicine is valued for its unique biomimetic bone matrix components. Icariin (Ica), which is claimed by Chinese medicine as a bone-repairing agent, is also widely accepted. In this report, a novel Ica-encapsulated PLGA/nano deer bone meal (Ica@PLGA/nBM) biomimetic microsphere was fabricated. The Ica could be released from microspheres in a controlled manner. In vitro results showed that the microsphere had good biocompatibility due to the addition of nBM and the MC3T3-E1 cells could adhere to and grow well on the microspheres. Moreover, Ica and nBM in microspheres could synergically promote the ALP activity of MC3T3-E1 cells significantly (p < 0.05), which indicated the acceleration of osteogenic differentiation. The RT-PCR results showed that the expression levels of bone matrix proteins (Col1 & OPN) were enhanced by the composite (p < 0.05). In addition, Ica and nBM in microspheres also obviously promoted the expression of RUNX2 and BMP-2, respectively (p < 0.05). The in vivo results demonstrated the effective promotion of Ica@PLGA/nBM on complete bony connection to cover the defect site and the bone defect repair. And the Ica(M)@PLGA/nBM microspheres provided the best repair results. All the results confirmed that the Ica@PLGA/nBM composite biomimetic microsphere has the potential for clinical application.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions.
Supporting Information
| Filename | Description |
|---|---|
| app55175-sup-0001-Supinfo.docxWord 2007 document , 93 KB | Figure S1 The standard curve of the relationship between absorbance values at 270 nm (OD270) and Ica concentration in solution. Figure S2. The FT-IR spectra of the composites near the characteristic peaks. Table S1. Primers used in qRT-PCR |
| app55175-sup-0002-VideoS1.mp4MPEG-4 video, 1.4 MB | Video S1: Supporting Information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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