Enzyme Catalysis in Non-Native Environment with Unnatural Selectivity Using Polymeric Nanoreactors
Dr. Jingjing Gao
Department of Chemistry, University of Massachusetts Amherst, Amherst, MA, 01003 USA
Center for Nanomedicine and Division of Engineering in Medicine, Department of Anesthesiology, Brigham and Women's Hospital, Boston, MA, 02115 USA
Harvard Medical School, Boston, MA, 02115 USA
Search for more papers by this authorStephanie Le
Department of Chemistry, University of Massachusetts Amherst, Amherst, MA, 01003 USA
Search for more papers by this authorCorresponding Author
Prof. Dr. S. Thayumanavan
Department of Chemistry, University of Massachusetts Amherst, Amherst, MA, 01003 USA
Search for more papers by this authorDr. Jingjing Gao
Department of Chemistry, University of Massachusetts Amherst, Amherst, MA, 01003 USA
Center for Nanomedicine and Division of Engineering in Medicine, Department of Anesthesiology, Brigham and Women's Hospital, Boston, MA, 02115 USA
Harvard Medical School, Boston, MA, 02115 USA
Search for more papers by this authorStephanie Le
Department of Chemistry, University of Massachusetts Amherst, Amherst, MA, 01003 USA
Search for more papers by this authorCorresponding Author
Prof. Dr. S. Thayumanavan
Department of Chemistry, University of Massachusetts Amherst, Amherst, MA, 01003 USA
Search for more papers by this authorGraphical Abstract
Amphiphilic random copolymers were employed to construct enzyme nanoreactors for catalysis in an organic solvent. Unnatural selectivity over a range of substrates was introduced by tuning the cross-linking density.
Abstract
The utilization of enzymes for catalysis in organic solvents, while exhibiting selectivity to different substrates, is a big challenge. We report an amphiphilic random copolymer system that self-assembles with enzymes in an organic solvent to form nanoreactors. These encapsulated enzymes are not denatured and they do preserve the catalytic activity. The cross-linkable functional groups in the hydrophobic compartments of the polymers offer to control accessibility to the enzyme. This varied accessibility due to the polymer host, rather than the enzyme itself, endows the nanoreactor with an unnatural selectivity. The findings here highlight the significant potential of simple polymer-based enzyme nanoreactors to execute selective organic reactions under non-native conditions.
Conflict of interest
The authors declare no conflict of interest.
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