Super-resolved Fluorescence Microscopy: Nobel Prize in Chemistry 2014 for Eric Betzig, Stefan Hell, and William E. Moerner†
M. Sc. Leonhard Möckl
Department for Chemistry and Center for NanoScience (CeNS), University of Munich (LMU), Butenandtstrasse 5–13 (E), 81377 Munich (Germany)
Search for more papers by this authorProf. Dr. Don C. Lamb
Department for Chemistry and Center for NanoScience (CeNS), University of Munich (LMU), Butenandtstrasse 5–13 (E), 81377 Munich (Germany)
Search for more papers by this authorCorresponding Author
Prof. Dr. Christoph Bräuchle
Department for Chemistry and Center for NanoScience (CeNS), University of Munich (LMU), Butenandtstrasse 5–13 (E), 81377 Munich (Germany)
Department for Chemistry and Center for NanoScience (CeNS), University of Munich (LMU), Butenandtstrasse 5–13 (E), 81377 Munich (Germany)Search for more papers by this authorM. Sc. Leonhard Möckl
Department for Chemistry and Center for NanoScience (CeNS), University of Munich (LMU), Butenandtstrasse 5–13 (E), 81377 Munich (Germany)
Search for more papers by this authorProf. Dr. Don C. Lamb
Department for Chemistry and Center for NanoScience (CeNS), University of Munich (LMU), Butenandtstrasse 5–13 (E), 81377 Munich (Germany)
Search for more papers by this authorCorresponding Author
Prof. Dr. Christoph Bräuchle
Department for Chemistry and Center for NanoScience (CeNS), University of Munich (LMU), Butenandtstrasse 5–13 (E), 81377 Munich (Germany)
Department for Chemistry and Center for NanoScience (CeNS), University of Munich (LMU), Butenandtstrasse 5–13 (E), 81377 Munich (Germany)Search for more papers by this authorWe gratefully acknowledge the assistance of Frauke Mickler and Philipp Messer.
Graphical Abstract
A big honor for small objects: The Nobel Prize in Chemistry 2014 was jointly awarded to Eric Betzig, Stefan Hell, and William E. Moerner “for the development of super-resolved fluorescence microscopy”. This Highlight describes how the field of super-resolution microscopy developed from the first detection of a single molecule in 1989 to the sophisticated techniques of today.
References
- 1E. Abbe, Arch. Mikrosk. Anat. Entwicklungsmech. 1873, 9, 413–418.
10.1007/BF02956173 Google Scholar
- 2M. A. Thompson, M. D. Lew, W. E. Moerner, Annu. Rev. Biophys. 2012, 41, 321–342.
- 3W. E. Moerner, L. Kador, Phys. Rev. Lett. 1989, 62, 2535–2538.
- 4
- 4aE. Betzig, Opt. Lett. 1995, 20, 237–239;
- 4bE. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, H. F. Hess, Science 2006, 313, 1642–1645.
- 5
- 5aS. W. Hell, J. Wichmann, Opt. Lett. 1994, 19, 780–782;
- 5bT. A. Klar, S. Jakobs, M. Dyba, A. Egner, S. W. Hell, Proc. Natl. Acad. Sci. USA 2000, 97, 8206–8210;
- 5cS. W. Hell, M. Kroug, Appl. Phys. B 1995, 60, 495–497.
- 6E. Schrödinger, Br. J. Phil. Sci. 1952, III, 233–242.
10.1093/bjps/III.11.233 Google Scholar
- 7M. Orrit, J. Bernard, Phys. Rev. Lett. 1990, 65, 2716–2719.
- 8W. E. Moerner, T. Basché, Angew. Chem. Int. Ed. Engl. 1993, 32, 457–476; Angew. Chem. 1993, 105, 537–557.
- 9E. B. Shera, N. K. Seitzinger, L. M. Davis, R. A. Keller, S. A. Soper, Chem. Phys. Lett. 1990, 174, 553–557.
- 10 Single Molecule Spectroscopy in Chemistry, Physics and Biology, (Nobel Symposium 2008) (Hrsg.: ), Springer, Heidelberg, 2010.
- 11A. Yildiz, J. N. Forkey, S. A. McKinney, T. Ha, Y. E. Goldman, P. R. Selvin, Science 2003, 300, 2061–2065.
- 12M. A. Thompson, J. M. Casolari, M. Badieirostami, P. O. Brown, W. E. Moerner, Proc. Natl. Acad. Sci. USA 2010, 107, 17864–17871.
- 13
- 13aC. E. Shannon, Proc. IRE 1949, 37, 10–21;
- 13bH. Nyquist, Trans. AIEE 1928, 47, 617–644.
- 14A. M. van Oijen, J. Kohler, J. Schmidt, M. Muller, G. J. Brakenhoff, Chem. Phys. Lett. 1998, 292.
- 15R. M. Dickson, A. B. Cubitt, R. Y. Tsien, W. E. Moerner, Nature 1997, 388, 355–358.
- 16G. H. Patterson, J. Lippincott-Schwartz, Science 2002, 297, 1873–1877.
- 17S. T. Hess, T. P. Girirajan, M. D. Mason, Biophys. J. 2006, 91, 4258–4272.
- 18M. J. Rust, M. Bates, X. Zhuang, Nat. Methods 2006, 3, 793–795.
- 19B. Huang, W. Wang, M. Bates, X. Zhuang, Science 2008, 319, 810–813.
- 20S. R. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, W. E. Moerner, Proc. Natl. Acad. Sci. USA 2009, 106, 2995–2999.
- 21H. Shroff, C. G. Galbraith, J. A. Galbraith, H. White, J. Gillette, S. Olenych, M. W. Davidson, E. Betzig, Proc. Natl. Acad. Sci. USA 2007, 104, 20308–20313.
- 22F. Huang, T. M. Hartwich, F. E. Rivera-Molina, Y. Lin, W. C. Duim, J. J. Long, P. D. Uchil, J. R. Myers, M. A. Baird, W. Mothes, M. W. Davidson, D. Toomre, J. Bewersdorf, Nat. Methods 2013, 10, 653–658.
- 23P. Török, P. R. T. Munro, Opt. Express 2004, 12, 3605–3617.
- 24D. Wildanger, R. Medda, L. Kastrup, S. W. Hell, J. Microsc. 2009, 236, 35–43.
- 25G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Luhrmann, R. Jahn, C. Eggeling, S. W. Hell, Proc. Natl. Acad. Sci. USA 2006, 103, 11440–11445.
- 26
- 26aG. Donnert, J. Keller, C. A. Wurm, S. O. Rizzoli, V. Westphal, A. Schonle, R. Jahn, S. Jakobs, C. Eggeling, S. W. Hell, Biophys. J. 2007, 92, L67–69;
- 26bJ. Bückers, D. Wildanger, G. Vicidomini, L. Kastrup, S. W. Hell, Opt. Express 2011, 19, 3130–3143.
- 27V. N. Belov, G. Y. Mitronova, M. L. Bossi, V. P. Boyarskiy, E. Hebisch, C. Geisler, K. Kolmakov, C. A. Wurm, K. I. Willig, S. W. Hell, Chem. Eur. J. 2014, 20, 13162—13173.
- 28
- 28aJ. R. Moffitt, C. Osseforth, J. Michaelis, Opt. Express 2011, 19, 4242–4254;
- 28bG. Vicidomini, G. Moneron, K. Y. Han, V. Westphal, H. Ta, M. Reuss, J. Engelhardt, C. Eggeling, S. W. Hell, Nat. Methods 2011, 8, 571–573.
- 29C. Eggeling, C. Ringemann, R. Medda, G. Schwarzmann, K. Sandhoff, S. Polyakova, V. N. Belov, B. Hein, C. von Middendorff, A. Schonle, S. W. Hell, Nature 2009, 457, 1159–1162.
- 30T. Grotjohann, I. Testa, M. Leutenegger, H. Bock, N. T. Urban, F. Lavoie-Cardinal, K. I. Willig, C. Eggeling, S. Jakobs, S. W. Hell, Nature 2011, 478, 204–208.
- 31J. L. Ptacin, S. F. Lee, E. C. Garner, E. Toro, M. Eckart, L. R. Comolli, W. E. Moerner, L. Shapiro, Nat. Cell Biol. 2010, 12, 791–798.
- 32S. J. Sahl, L. E. Weiss, W. C. Duim, J. Frydman, W. E. Moerner, Sci. Rep. 2012, 2, 895.
- 33F. Göttfert, C. A. Wurm, V. Mueller, S. Berning, V. C. Cordes, A. Honigmann, S. W. Hell, Biophys. J. 2013, 105, p L01–L03.
- 34K. I. Willig, H. Steffens, C. Gregor, A. Herholt, M. J. Rossner, S. W. Hell, Biophys. J. 2014, 106, L01–03.
- 35
- 35aA. Gahlmann, W. E. Moerner, Nat. Rev. Microbiol. 2014, 12, 9–22;
- 35bW. E. Moerner, Proc. Natl. Acad. Sci. USA 2007, 104, 12596–12602.
- 36S. J. Sahl, W. E. Moerner, Curr. Opin. Struct. Biol. 2013, 23, 778–787.
