Dissolution-Enhanced Luminescent Bioassay Based on Inorganic Lanthanide Nanoparticles†
Shanyong Zhou
Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
State Key Laboratory of Structural Chemistry and Danish-Chinese Centre for Proteases and Cancer, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorDr. Wei Zheng
Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorDr. Zhuo Chen
State Key Laboratory of Structural Chemistry and Danish-Chinese Centre for Proteases and Cancer, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorDr. Datao Tu
Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorDr. Yongsheng Liu
Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorEn Ma
Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorRenfu Li
Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorDr. Haomiao Zhu
Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorCorresponding Author
Prof. Mingdong Huang
State Key Laboratory of Structural Chemistry and Danish-Chinese Centre for Proteases and Cancer, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Mingdong Huang, State Key Laboratory of Structural Chemistry and Danish-Chinese Centre for Proteases and Cancer, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Xueyuan Chen, Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorCorresponding Author
Prof. Xueyuan Chen
Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
State Key Laboratory of Structural Chemistry and Danish-Chinese Centre for Proteases and Cancer, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Mingdong Huang, State Key Laboratory of Structural Chemistry and Danish-Chinese Centre for Proteases and Cancer, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Xueyuan Chen, Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorShanyong Zhou
Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
State Key Laboratory of Structural Chemistry and Danish-Chinese Centre for Proteases and Cancer, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorDr. Wei Zheng
Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorDr. Zhuo Chen
State Key Laboratory of Structural Chemistry and Danish-Chinese Centre for Proteases and Cancer, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorDr. Datao Tu
Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorDr. Yongsheng Liu
Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorEn Ma
Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorRenfu Li
Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorDr. Haomiao Zhu
Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorCorresponding Author
Prof. Mingdong Huang
State Key Laboratory of Structural Chemistry and Danish-Chinese Centre for Proteases and Cancer, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Mingdong Huang, State Key Laboratory of Structural Chemistry and Danish-Chinese Centre for Proteases and Cancer, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Xueyuan Chen, Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorCorresponding Author
Prof. Xueyuan Chen
Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
State Key Laboratory of Structural Chemistry and Danish-Chinese Centre for Proteases and Cancer, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Mingdong Huang, State Key Laboratory of Structural Chemistry and Danish-Chinese Centre for Proteases and Cancer, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Xueyuan Chen, Key Laboratory of Optoelectronic Materials Chemistry and Physics and Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002 (China)
Search for more papers by this authorWe thank Prof. Weiping Su for helpful discussions and Dr. Xianren Ye in Fujian Provincial Cancer Hospital for kindly providing us with the human serum samples. This work is supported by the 973 program of MOST (No. 2014CB845605), Special Project of National Major Scientific Equipment Development of China (No. 2012YQ120060), the NSFC (Nos. 11204302, 11304314, U1305244, and 21325104), the Key Project of Science and Technology Foundation of Fujian Province (No. 2013H0060), the CAS/SAFEA International Partnership Program for Creative Research Teams, and Strategic Priority Research Program of the CAS (No. XDA09030307).
Graphical Abstract
An ultrasensitive bioassay, based on the dissolution-enhanced luminescence of inorganic lanthanide (Ln3+) nanoparticles (NPs), was developed for the detection of carcinoembryonic antigen in human serum samples. As a result of the high Ln3+ labeling ratio, the detection limit is improved with a record-low value of 0.1 pg mL−1 (0.5 fM) compared to a commercial dissociation-enhanced Ln3+ fluoroimmunoassay.
Abstract
Conventional dissociation-enhanced lanthanide fluoroimmunoassays (DELFIA) using molecular probes suffer from a low labeling ratio of lanthanide ions (Ln3+) per biomolecule. Herein, we develop a unique bioassay based on the dissolution-enhanced luminescence of inorganic lanthanide nanoparticles (NPs). As a result of the highly concentrated Ln3+ ions in a single Ln3+ NP, an extremely high Ln3+ labeling ratio can be achieved, which amplifies significantly the luminescence signal and thus improves the detection sensitivity compared to DELFIA. Utilizing sub-10 nm NaEuF4 NPs as dissolution-enhanced luminescent nanoprobes, we demonstrate the successful in vitro detection of carcinoembryonic antigen (CEA, an important tumor marker) in human serum samples with a record-low detection limit of 0.1 pg mL−1 (0.5 fM). This value is an improvement of approximately 3 orders of magnitude relative to that of DELFIA. The dissolution-enhanced luminescent bioassay shows great promise in versatile bioapplications, such as ultrasensitive and multiplexed in vitro detection of disease markers in clinical diagnosis.
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Citing Literature
Special Issue:Nanotechnology & Nanomaterials, Nanotoxicology & Nanomedicine
November 10, 2014
Pages 12498-12502
