Communication

Ligand-Induced Conformational Changes of the Multidrug Resistance Transporter EmrE Probed by Oriented Solid-State NMR Spectroscopy^†

Dr. Anindita Gayen

Department of Chemistry, New York University, 100 Washington Square East, New York, NY 10003 (USA) http://www.nyu.edu/fas/dept/chemistry/traasethgroup/

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James R. Banigan,

James R. Banigan

Department of Chemistry, New York University, 100 Washington Square East, New York, NY 10003 (USA) http://www.nyu.edu/fas/dept/chemistry/traasethgroup/

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Prof. Nathaniel J. Traaseth,

Corresponding Author

Prof. Nathaniel J. Traaseth

[email protected]

Department of Chemistry, New York University, 100 Washington Square East, New York, NY 10003 (USA) http://www.nyu.edu/fas/dept/chemistry/traasethgroup/

Department of Chemistry, New York University, 100 Washington Square East, New York, NY 10003 (USA) http://www.nyu.edu/fas/dept/chemistry/traasethgroup/Search for more papers by this author

Dr. Anindita Gayen,

Dr. Anindita Gayen

Department of Chemistry, New York University, 100 Washington Square East, New York, NY 10003 (USA) http://www.nyu.edu/fas/dept/chemistry/traasethgroup/

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James R. Banigan,

James R. Banigan

Department of Chemistry, New York University, 100 Washington Square East, New York, NY 10003 (USA) http://www.nyu.edu/fas/dept/chemistry/traasethgroup/

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Prof. Nathaniel J. Traaseth,

Corresponding Author

Prof. Nathaniel J. Traaseth

[email protected]

Department of Chemistry, New York University, 100 Washington Square East, New York, NY 10003 (USA) http://www.nyu.edu/fas/dept/chemistry/traasethgroup/

Department of Chemistry, New York University, 100 Washington Square East, New York, NY 10003 (USA) http://www.nyu.edu/fas/dept/chemistry/traasethgroup/Search for more papers by this author

First published: 12 August 2013

https://doi.org/10.1002/anie.201303091

Citations: 56

^†

This work was supported by NIH grant K22AI083745 and start-up funds from New York University. We thank Prof. Ray Turner and Dr. Denice Bay for helpful discussions, Tracy Stanzel for technical assistance, the National High Magnetic Field Laboratory for preliminary NMR experiments, and Prof. Bobby Arora and Steve Joy for synthesis of BMPS.

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Graphical Abstract

An EmrE-ging market: Oriented solid-state NMR spectroscopy and biochemical cross-linking experiments were used to show that the ligand-free membrane protein transporter EmrE forms anti-parallel dimers with different monomer tilt angles relative to the lipid bilayer. In addition, subtle conformational changes were detected upon drug binding that emphasize the need for an atomic-resolution structure.

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Volume52, Issue39

September 23, 2013

Pages 10321-10324

Ligand-Induced Conformational Changes of the Multidrug Resistance Transporter EmrE Probed by Oriented Solid-State NMR Spectroscopy^†

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Ligand-Induced Conformational Changes of the Multidrug Resistance Transporter EmrE Probed by Oriented Solid-State NMR Spectroscopy†

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Ligand-Induced Conformational Changes of the Multidrug Resistance Transporter EmrE Probed by Oriented Solid-State NMR Spectroscopy^†