Rapid Removal of Matrices from Small-Volume Samples by Step-Voltage Nanoelectrospray†
Zhenwei Wei
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
Search for more papers by this authorShuo Han
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
Search for more papers by this authorXiaoyun Gong
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
Search for more papers by this authorYaoyao Zhao
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
Search for more papers by this authorChengdui Yang
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
Search for more papers by this authorProf. Sichun Zhang
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
Search for more papers by this authorCorresponding Author
Prof. Xinrong Zhang
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)Search for more papers by this authorZhenwei Wei
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
Search for more papers by this authorShuo Han
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
Search for more papers by this authorXiaoyun Gong
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
Search for more papers by this authorYaoyao Zhao
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
Search for more papers by this authorChengdui Yang
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
Search for more papers by this authorProf. Sichun Zhang
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
Search for more papers by this authorCorresponding Author
Prof. Xinrong Zhang
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)
Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084 (China)Search for more papers by this authorThis research is supported by the 973 program (2013CB933800), the National Natural Science Foundation of China (No. 21027013) and the Ministry of Science and Technology of China (No. 2011YQ090005).
Graphical Abstract
Matrix unloaded: By changing from fixed-voltage (left) to step-voltage nanoelectrospray (right), the mass-spectrometric analysis of small-volume physiological samples is possible. Separation and ionization are achieved in one process, which avoids sample loss and dilution and prevents interference by the matrix. The result is high sensitivity even for samples at the nanoliter level.
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